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Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection
Salmonellosis is a gastrointestinal disease caused by non-typhoidal Salmonella serovars such as Salmonella Typhimurium. This pathology is a zoonosis, and food animals with subclinical infection constitute a vast reservoir for disease. After intestinal colonization, Salmonella Typhimurium reaches mes...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796392/ https://www.ncbi.nlm.nih.gov/pubmed/29391047 http://dx.doi.org/10.1186/s13567-018-0506-1 |
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author | Herrera-Uribe, Juber Zaldívar-López, Sara Aguilar, Carmen Luque, Cristina Bautista, Rocío Carvajal, Ana Claros, M. Gonzalo Garrido, Juan J. |
author_facet | Herrera-Uribe, Juber Zaldívar-López, Sara Aguilar, Carmen Luque, Cristina Bautista, Rocío Carvajal, Ana Claros, M. Gonzalo Garrido, Juan J. |
author_sort | Herrera-Uribe, Juber |
collection | PubMed |
description | Salmonellosis is a gastrointestinal disease caused by non-typhoidal Salmonella serovars such as Salmonella Typhimurium. This pathology is a zoonosis, and food animals with subclinical infection constitute a vast reservoir for disease. After intestinal colonization, Salmonella Typhimurium reaches mesenteric lymph nodes (MLN), where infection is controlled avoiding systemic spread. Although the molecular basis of this infection has been extensively studied, little is known about how microRNA (miRNA) regulate the expression of proteins involved in the Salmonella-host interaction. Using small RNA-seq, we examined expression profiles of MLN 2 days after infection with Salmonella Typhimurium, and we found 110 dysregulated miRNA. Among them, we found upregulated miR-21, miR-155, miR-150, and miR-221, as well as downregulated miR-143 and miR-125, all of them previously linked to other bacterial infections. Integration with proteomic data revealed 30 miRNA potentially regulating the expression of 15 proteins involved in biological functions such as cell death and survival, inflammatory response and antigenic presentation. The inflammatory response was found increased via upregulation of miRNA such as miR-21 and miR-155. Downregulation of miR-125a/b, miR-148 and miR-1 were identified as potential regulators of MHC-class I components PSMB8, HSP90B1 and PDIA3, respectively. Furthermore, we confirmed that miR-125a is a direct target of immunoproteasome component PSMB8. Since we also found miR-130 downregulation, which is associated with upregulation of HSPA8, we suggest induction of both MHC-I and MHC-II antigen presentation pathways. In conclusion, our study identifies miRNA that could regulate critical networks for antigenic presentation, inflammatory response and cytoskeletal rearrangements. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13567-018-0506-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5796392 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57963922018-02-12 Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection Herrera-Uribe, Juber Zaldívar-López, Sara Aguilar, Carmen Luque, Cristina Bautista, Rocío Carvajal, Ana Claros, M. Gonzalo Garrido, Juan J. Vet Res Research Article Salmonellosis is a gastrointestinal disease caused by non-typhoidal Salmonella serovars such as Salmonella Typhimurium. This pathology is a zoonosis, and food animals with subclinical infection constitute a vast reservoir for disease. After intestinal colonization, Salmonella Typhimurium reaches mesenteric lymph nodes (MLN), where infection is controlled avoiding systemic spread. Although the molecular basis of this infection has been extensively studied, little is known about how microRNA (miRNA) regulate the expression of proteins involved in the Salmonella-host interaction. Using small RNA-seq, we examined expression profiles of MLN 2 days after infection with Salmonella Typhimurium, and we found 110 dysregulated miRNA. Among them, we found upregulated miR-21, miR-155, miR-150, and miR-221, as well as downregulated miR-143 and miR-125, all of them previously linked to other bacterial infections. Integration with proteomic data revealed 30 miRNA potentially regulating the expression of 15 proteins involved in biological functions such as cell death and survival, inflammatory response and antigenic presentation. The inflammatory response was found increased via upregulation of miRNA such as miR-21 and miR-155. Downregulation of miR-125a/b, miR-148 and miR-1 were identified as potential regulators of MHC-class I components PSMB8, HSP90B1 and PDIA3, respectively. Furthermore, we confirmed that miR-125a is a direct target of immunoproteasome component PSMB8. Since we also found miR-130 downregulation, which is associated with upregulation of HSPA8, we suggest induction of both MHC-I and MHC-II antigen presentation pathways. In conclusion, our study identifies miRNA that could regulate critical networks for antigenic presentation, inflammatory response and cytoskeletal rearrangements. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13567-018-0506-1) contains supplementary material, which is available to authorized users. BioMed Central 2018-02-01 2018 /pmc/articles/PMC5796392/ /pubmed/29391047 http://dx.doi.org/10.1186/s13567-018-0506-1 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Herrera-Uribe, Juber Zaldívar-López, Sara Aguilar, Carmen Luque, Cristina Bautista, Rocío Carvajal, Ana Claros, M. Gonzalo Garrido, Juan J. Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection |
title | Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection |
title_full | Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection |
title_fullStr | Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection |
title_full_unstemmed | Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection |
title_short | Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection |
title_sort | regulatory role of microrna in mesenteric lymph nodes after salmonella typhimurium infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796392/ https://www.ncbi.nlm.nih.gov/pubmed/29391047 http://dx.doi.org/10.1186/s13567-018-0506-1 |
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