Cargando…
Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
OBJECTIVE: Loop-mediated isothermal amplification (LAMP) of DNA is gaining relevance as a method to detect nucleic acids, as it is easier, faster, and more powerful than conventional Polymerase Chain Reaction. However, LAMP is still mostly used in laboratory settings, because of the lack of a cheap...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796575/ https://www.ncbi.nlm.nih.gov/pubmed/29391052 http://dx.doi.org/10.1186/s13104-018-3197-9 |
_version_ | 1783297525997895680 |
---|---|
author | Velders, Aldrik H. Schoen, Cor Saggiomo, Vittorio |
author_facet | Velders, Aldrik H. Schoen, Cor Saggiomo, Vittorio |
author_sort | Velders, Aldrik H. |
collection | PubMed |
description | OBJECTIVE: Loop-mediated isothermal amplification (LAMP) of DNA is gaining relevance as a method to detect nucleic acids, as it is easier, faster, and more powerful than conventional Polymerase Chain Reaction. However, LAMP is still mostly used in laboratory settings, because of the lack of a cheap and easy, one-button device that can perform LAMP experiments. RESULTS: Here we show how to build and program an Arduino shield for a LAMP and detection of DNA. The here described Arduino Shield is cheap, easy to assemble, to program and use, it is battery operated and the detection of DNA is done by naked-eye so that it can be used in field. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3197-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5796575 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-57965752018-02-12 Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection Velders, Aldrik H. Schoen, Cor Saggiomo, Vittorio BMC Res Notes Research Note OBJECTIVE: Loop-mediated isothermal amplification (LAMP) of DNA is gaining relevance as a method to detect nucleic acids, as it is easier, faster, and more powerful than conventional Polymerase Chain Reaction. However, LAMP is still mostly used in laboratory settings, because of the lack of a cheap and easy, one-button device that can perform LAMP experiments. RESULTS: Here we show how to build and program an Arduino shield for a LAMP and detection of DNA. The here described Arduino Shield is cheap, easy to assemble, to program and use, it is battery operated and the detection of DNA is done by naked-eye so that it can be used in field. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3197-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-02-01 /pmc/articles/PMC5796575/ /pubmed/29391052 http://dx.doi.org/10.1186/s13104-018-3197-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Note Velders, Aldrik H. Schoen, Cor Saggiomo, Vittorio Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection |
title | Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection |
title_full | Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection |
title_fullStr | Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection |
title_full_unstemmed | Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection |
title_short | Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection |
title_sort | loop-mediated isothermal amplification (lamp) shield for arduino dna detection |
topic | Research Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796575/ https://www.ncbi.nlm.nih.gov/pubmed/29391052 http://dx.doi.org/10.1186/s13104-018-3197-9 |
work_keys_str_mv | AT veldersaldrikh loopmediatedisothermalamplificationlampshieldforarduinodnadetection AT schoencor loopmediatedisothermalamplificationlampshieldforarduinodnadetection AT saggiomovittorio loopmediatedisothermalamplificationlampshieldforarduinodnadetection |