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Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection

OBJECTIVE: Loop-mediated isothermal amplification (LAMP) of DNA is gaining relevance as a method to detect nucleic acids, as it is easier, faster, and more powerful than conventional Polymerase Chain Reaction. However, LAMP is still mostly used in laboratory settings, because of the lack of a cheap...

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Autores principales: Velders, Aldrik H., Schoen, Cor, Saggiomo, Vittorio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796575/
https://www.ncbi.nlm.nih.gov/pubmed/29391052
http://dx.doi.org/10.1186/s13104-018-3197-9
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author Velders, Aldrik H.
Schoen, Cor
Saggiomo, Vittorio
author_facet Velders, Aldrik H.
Schoen, Cor
Saggiomo, Vittorio
author_sort Velders, Aldrik H.
collection PubMed
description OBJECTIVE: Loop-mediated isothermal amplification (LAMP) of DNA is gaining relevance as a method to detect nucleic acids, as it is easier, faster, and more powerful than conventional Polymerase Chain Reaction. However, LAMP is still mostly used in laboratory settings, because of the lack of a cheap and easy, one-button device that can perform LAMP experiments. RESULTS: Here we show how to build and program an Arduino shield for a LAMP and detection of DNA. The here described Arduino Shield is cheap, easy to assemble, to program and use, it is battery operated and the detection of DNA is done by naked-eye so that it can be used in field. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3197-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-57965752018-02-12 Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection Velders, Aldrik H. Schoen, Cor Saggiomo, Vittorio BMC Res Notes Research Note OBJECTIVE: Loop-mediated isothermal amplification (LAMP) of DNA is gaining relevance as a method to detect nucleic acids, as it is easier, faster, and more powerful than conventional Polymerase Chain Reaction. However, LAMP is still mostly used in laboratory settings, because of the lack of a cheap and easy, one-button device that can perform LAMP experiments. RESULTS: Here we show how to build and program an Arduino shield for a LAMP and detection of DNA. The here described Arduino Shield is cheap, easy to assemble, to program and use, it is battery operated and the detection of DNA is done by naked-eye so that it can be used in field. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13104-018-3197-9) contains supplementary material, which is available to authorized users. BioMed Central 2018-02-01 /pmc/articles/PMC5796575/ /pubmed/29391052 http://dx.doi.org/10.1186/s13104-018-3197-9 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Velders, Aldrik H.
Schoen, Cor
Saggiomo, Vittorio
Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
title Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
title_full Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
title_fullStr Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
title_full_unstemmed Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
title_short Loop-mediated isothermal amplification (LAMP) shield for Arduino DNA detection
title_sort loop-mediated isothermal amplification (lamp) shield for arduino dna detection
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796575/
https://www.ncbi.nlm.nih.gov/pubmed/29391052
http://dx.doi.org/10.1186/s13104-018-3197-9
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