Lactobacillus gasseri CRISPR-Cas9 characterization In Vitro reveals a flexible mode of protospacer-adjacent motif recognition

While the CRISPR-Cas9 system from S. pyogenes is a powerful genome engineering tool, additional programmed nucleases would enable added flexibility in targeting space and multiplexing. Here, we characterized a CRISPR-Cas9 system from L. gasseri and found that it has modest activity in a cell-free ly...

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Detalles Bibliográficos
Autores principales: Anderson, Emily M., McClelland, Shawn, Maksimova, Elena, Strezoska, Žaklina, Basila, Megan, Briner, Alexandra E., Barrangou, Rodolphe, Smith, Anja van Brabant
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796720/
https://www.ncbi.nlm.nih.gov/pubmed/29394276
http://dx.doi.org/10.1371/journal.pone.0192181

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796720/
https://www.ncbi.nlm.nih.gov/pubmed/29394276
http://dx.doi.org/10.1371/journal.pone.0192181

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