Cargando…
Analysis of Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Bacterial Wilt in Korea
The Ralstonia solanacearum species complex (RSSC) can be divided into four phylotypes, and includes phenotypically diverse bacterial strains that cause bacterial wilt on various host plants. This study used 93 RSSC isolates responsible for potato bacterial wilt in Korea, and investigated their phylo...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Plant Pathology
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796747/ https://www.ncbi.nlm.nih.gov/pubmed/29422785 http://dx.doi.org/10.5423/PPJ.FT.09.2017.0203 |
_version_ | 1783297555721879552 |
---|---|
author | Cho, Heejung Song, Eun-Sung Lee, Young Kee Lee, Seungdon Lee, Seon-Woo Jo, Ara Lee, Byoung-Moo Kim, Jeong-Gu Hwang, Ingyu |
author_facet | Cho, Heejung Song, Eun-Sung Lee, Young Kee Lee, Seungdon Lee, Seon-Woo Jo, Ara Lee, Byoung-Moo Kim, Jeong-Gu Hwang, Ingyu |
author_sort | Cho, Heejung |
collection | PubMed |
description | The Ralstonia solanacearum species complex (RSSC) can be divided into four phylotypes, and includes phenotypically diverse bacterial strains that cause bacterial wilt on various host plants. This study used 93 RSSC isolates responsible for potato bacterial wilt in Korea, and investigated their phylogenetic relatedness based on the analysis of phylotype, biovar, and host range. Of the 93 isolates, twenty-two were identified as biovar 2, eight as biovar 3, and sixty-three as biovar 4. Applied to the phylotype scheme, biovar 3 and 4 isolates belonged to phylotype I, and biovar 2 isolates belonged to phylotype IV. This classification was consistent with phylogenetic trees based on 16S rRNA and egl gene sequences, in which biovar 3 and 4 isolates clustered to phylotype I, and biovar 2 isolates clustered to phylotype IV. Korean biovar 2 isolates were distinct from biovar 3 and 4 isolates pathologically as well as genetically - all biovar 2 isolates were nonpathogenic to peppers. Additionally, in host-determining assays, we found uncommon strains among biovar 2 of phylotype IV, which were the tomato-nonpathogenic strains. Since tomatoes are known to be highly susceptible to RSSC, to the best of our knowledge this is the first report of tomato-nonpathogenic potato strains. These results imply the potential prevalence of greater RSSC diversity in terms of host range than would be predicted based on phylogenetic analysis. |
format | Online Article Text |
id | pubmed-5796747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Korean Society of Plant Pathology |
record_format | MEDLINE/PubMed |
spelling | pubmed-57967472018-02-08 Analysis of Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Bacterial Wilt in Korea Cho, Heejung Song, Eun-Sung Lee, Young Kee Lee, Seungdon Lee, Seon-Woo Jo, Ara Lee, Byoung-Moo Kim, Jeong-Gu Hwang, Ingyu Plant Pathol J Research Article The Ralstonia solanacearum species complex (RSSC) can be divided into four phylotypes, and includes phenotypically diverse bacterial strains that cause bacterial wilt on various host plants. This study used 93 RSSC isolates responsible for potato bacterial wilt in Korea, and investigated their phylogenetic relatedness based on the analysis of phylotype, biovar, and host range. Of the 93 isolates, twenty-two were identified as biovar 2, eight as biovar 3, and sixty-three as biovar 4. Applied to the phylotype scheme, biovar 3 and 4 isolates belonged to phylotype I, and biovar 2 isolates belonged to phylotype IV. This classification was consistent with phylogenetic trees based on 16S rRNA and egl gene sequences, in which biovar 3 and 4 isolates clustered to phylotype I, and biovar 2 isolates clustered to phylotype IV. Korean biovar 2 isolates were distinct from biovar 3 and 4 isolates pathologically as well as genetically - all biovar 2 isolates were nonpathogenic to peppers. Additionally, in host-determining assays, we found uncommon strains among biovar 2 of phylotype IV, which were the tomato-nonpathogenic strains. Since tomatoes are known to be highly susceptible to RSSC, to the best of our knowledge this is the first report of tomato-nonpathogenic potato strains. These results imply the potential prevalence of greater RSSC diversity in terms of host range than would be predicted based on phylogenetic analysis. Korean Society of Plant Pathology 2018-02 2018-02-01 /pmc/articles/PMC5796747/ /pubmed/29422785 http://dx.doi.org/10.5423/PPJ.FT.09.2017.0203 Text en © The Korean Society of Plant Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cho, Heejung Song, Eun-Sung Lee, Young Kee Lee, Seungdon Lee, Seon-Woo Jo, Ara Lee, Byoung-Moo Kim, Jeong-Gu Hwang, Ingyu Analysis of Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Bacterial Wilt in Korea |
title | Analysis of Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Bacterial Wilt in Korea |
title_full | Analysis of Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Bacterial Wilt in Korea |
title_fullStr | Analysis of Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Bacterial Wilt in Korea |
title_full_unstemmed | Analysis of Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Bacterial Wilt in Korea |
title_short | Analysis of Genetic and Pathogenic Diversity of Ralstonia solanacearum Causing Potato Bacterial Wilt in Korea |
title_sort | analysis of genetic and pathogenic diversity of ralstonia solanacearum causing potato bacterial wilt in korea |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796747/ https://www.ncbi.nlm.nih.gov/pubmed/29422785 http://dx.doi.org/10.5423/PPJ.FT.09.2017.0203 |
work_keys_str_mv | AT choheejung analysisofgeneticandpathogenicdiversityofralstoniasolanacearumcausingpotatobacterialwiltinkorea AT songeunsung analysisofgeneticandpathogenicdiversityofralstoniasolanacearumcausingpotatobacterialwiltinkorea AT leeyoungkee analysisofgeneticandpathogenicdiversityofralstoniasolanacearumcausingpotatobacterialwiltinkorea AT leeseungdon analysisofgeneticandpathogenicdiversityofralstoniasolanacearumcausingpotatobacterialwiltinkorea AT leeseonwoo analysisofgeneticandpathogenicdiversityofralstoniasolanacearumcausingpotatobacterialwiltinkorea AT joara analysisofgeneticandpathogenicdiversityofralstoniasolanacearumcausingpotatobacterialwiltinkorea AT leebyoungmoo analysisofgeneticandpathogenicdiversityofralstoniasolanacearumcausingpotatobacterialwiltinkorea AT kimjeonggu analysisofgeneticandpathogenicdiversityofralstoniasolanacearumcausingpotatobacterialwiltinkorea AT hwangingyu analysisofgeneticandpathogenicdiversityofralstoniasolanacearumcausingpotatobacterialwiltinkorea |