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Core Fucosylation of the T Cell Receptor Is Required for T Cell Activation
CD4(+) T cell activation promotes the pathogenic process of systemic lupus erythematosus (SLE). T cell receptor (TCR) complex are highly core fucosylated glycoproteins, which play important roles in T cell activation. In this study, we found that the core fucosylation of CD4(+) T cells was significa...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796888/ https://www.ncbi.nlm.nih.gov/pubmed/29434598 http://dx.doi.org/10.3389/fimmu.2018.00078 |
Sumario: | CD4(+) T cell activation promotes the pathogenic process of systemic lupus erythematosus (SLE). T cell receptor (TCR) complex are highly core fucosylated glycoproteins, which play important roles in T cell activation. In this study, we found that the core fucosylation of CD4(+) T cells was significantly increased in SLE patients. Loss of core fucosyltransferase (Fut8), the sole enzyme for catalyzing the core fucosylation of N-glycan, significantly reduced CD4(+) T cell activation and ameliorated the experimental autoimmune encephalomyelitis-induced syndrome in Fut8(−/−) mice. T cell activation with OVA(323–339) loaded major histocompatibility complex II (pMHC-II) on B cell was dramatically attenuated in Fut8(−/−)OT-II CD4(+) T cells compared with Fut8(+/+)OT-II CD4(+) T cells. Moreover, the phosphorylation of ZAP-70 was significantly reduced in Fut8(+/+)OT-II CD4(+) T cells by the treatment of fucosidase. Our results suggest that core fucosylation is required for efficient TCR–pMHC-II contacts in CD4(+) T cell activation, and hyper core fucosylation may serve as a potential novel biomarker in the sera from SLE patients. |
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