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AhGLK1 affects chlorophyll biosynthesis and photosynthesis in peanut leaves during recovery from drought
Peanut is an important edible oil crop plant whose quality and yield are greatly affected by drought. The process and molecular mechanisms of recovery from drought are also critical to its productivity, but are currently poorly characterized. Here, we investigate the involvement of peanut AhGLK1 in...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796971/ https://www.ncbi.nlm.nih.gov/pubmed/29396501 http://dx.doi.org/10.1038/s41598-018-20542-7 |
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author | Liu, Xing Li, Limei Li, Meijuan Su, Liangchen Lian, Siman Zhang, Baihong Li, Xiaoyun Ge, Kui Li, Ling |
author_facet | Liu, Xing Li, Limei Li, Meijuan Su, Liangchen Lian, Siman Zhang, Baihong Li, Xiaoyun Ge, Kui Li, Ling |
author_sort | Liu, Xing |
collection | PubMed |
description | Peanut is an important edible oil crop plant whose quality and yield are greatly affected by drought. The process and molecular mechanisms of recovery from drought are also critical to its productivity, but are currently poorly characterized. Here, we investigate the involvement of peanut AhGLK1 in recovery from drought, and in particular its relationship with AhPORA, which encodes a key enzyme in chlorophyll biosynthesis. We found that chlorophyll content, chlorophyll fluorescence, AhPORA protein level and genes related to chlorophyll biosynthesis and photosynthesis declined markedly under drought conditions, but all increased during recovery. Consistent with this, AhGLK1 expression decreased during water stress and increased when the stress was removed. When AhGLK1 was transformed into Arabidopsis glk1glk2 mutant, it increased the survival rate of the mutant during recovery from drought and fully rescued the mutant’s pale-green phenotype. In addition, chlorophyll content and fluorescence, and the expression of genes related to chlorophyll biosynthesis and photosynthesis, were all increased. Bioinformatics analysis and experimental evidence suggested that AhGLK1 augments the expression of AhPORA by binding to its promoter. Our findings confirm that AhGLK1 plays a role as a transcription factor that upregulates expression of AhPORA during post-drought recovery, thereby stimulating chlorophyll biosynthesis and photosynthesis. |
format | Online Article Text |
id | pubmed-5796971 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57969712018-02-12 AhGLK1 affects chlorophyll biosynthesis and photosynthesis in peanut leaves during recovery from drought Liu, Xing Li, Limei Li, Meijuan Su, Liangchen Lian, Siman Zhang, Baihong Li, Xiaoyun Ge, Kui Li, Ling Sci Rep Article Peanut is an important edible oil crop plant whose quality and yield are greatly affected by drought. The process and molecular mechanisms of recovery from drought are also critical to its productivity, but are currently poorly characterized. Here, we investigate the involvement of peanut AhGLK1 in recovery from drought, and in particular its relationship with AhPORA, which encodes a key enzyme in chlorophyll biosynthesis. We found that chlorophyll content, chlorophyll fluorescence, AhPORA protein level and genes related to chlorophyll biosynthesis and photosynthesis declined markedly under drought conditions, but all increased during recovery. Consistent with this, AhGLK1 expression decreased during water stress and increased when the stress was removed. When AhGLK1 was transformed into Arabidopsis glk1glk2 mutant, it increased the survival rate of the mutant during recovery from drought and fully rescued the mutant’s pale-green phenotype. In addition, chlorophyll content and fluorescence, and the expression of genes related to chlorophyll biosynthesis and photosynthesis, were all increased. Bioinformatics analysis and experimental evidence suggested that AhGLK1 augments the expression of AhPORA by binding to its promoter. Our findings confirm that AhGLK1 plays a role as a transcription factor that upregulates expression of AhPORA during post-drought recovery, thereby stimulating chlorophyll biosynthesis and photosynthesis. Nature Publishing Group UK 2018-02-02 /pmc/articles/PMC5796971/ /pubmed/29396501 http://dx.doi.org/10.1038/s41598-018-20542-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Liu, Xing Li, Limei Li, Meijuan Su, Liangchen Lian, Siman Zhang, Baihong Li, Xiaoyun Ge, Kui Li, Ling AhGLK1 affects chlorophyll biosynthesis and photosynthesis in peanut leaves during recovery from drought |
title | AhGLK1 affects chlorophyll biosynthesis and photosynthesis in peanut leaves during recovery from drought |
title_full | AhGLK1 affects chlorophyll biosynthesis and photosynthesis in peanut leaves during recovery from drought |
title_fullStr | AhGLK1 affects chlorophyll biosynthesis and photosynthesis in peanut leaves during recovery from drought |
title_full_unstemmed | AhGLK1 affects chlorophyll biosynthesis and photosynthesis in peanut leaves during recovery from drought |
title_short | AhGLK1 affects chlorophyll biosynthesis and photosynthesis in peanut leaves during recovery from drought |
title_sort | ahglk1 affects chlorophyll biosynthesis and photosynthesis in peanut leaves during recovery from drought |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796971/ https://www.ncbi.nlm.nih.gov/pubmed/29396501 http://dx.doi.org/10.1038/s41598-018-20542-7 |
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