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LncRNA MALAT1 regulates smooth muscle cell phenotype switch via activation of autophagy

Vascular smooth muscle cells (VSMCs), switching from a differentiated to a proliferative phenotype, contribute to various vascular diseases. However, the role of long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 MALAT1 in the phenotype switching of VSMCs remains unclear. Here...

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Autores principales: Song, Tie-Feng, Huang, Li-Wen, Yuan, Ying, Wang, Hui-qin, He, Hong-Peng, Ma, Wen-Jian, Huo, Li-Hong, Zhou, Hao, Wang, Nan, Zhang, Tong-Cun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796983/
https://www.ncbi.nlm.nih.gov/pubmed/29435112
http://dx.doi.org/10.18632/oncotarget.23230
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author Song, Tie-Feng
Huang, Li-Wen
Yuan, Ying
Wang, Hui-qin
He, Hong-Peng
Ma, Wen-Jian
Huo, Li-Hong
Zhou, Hao
Wang, Nan
Zhang, Tong-Cun
author_facet Song, Tie-Feng
Huang, Li-Wen
Yuan, Ying
Wang, Hui-qin
He, Hong-Peng
Ma, Wen-Jian
Huo, Li-Hong
Zhou, Hao
Wang, Nan
Zhang, Tong-Cun
author_sort Song, Tie-Feng
collection PubMed
description Vascular smooth muscle cells (VSMCs), switching from a differentiated to a proliferative phenotype, contribute to various vascular diseases. However, the role of long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 MALAT1 in the phenotype switching of VSMCs remains unclear. Here, we report that the knockdown of MALAT1 promotes the transformation of smooth muscle cells from a proliferative phenotype to a differentiated phenotype. MALAT1 knockdown inhibited cellular proliferation and migration, leading to significant cell cycle arrest in the G2 phase. MALAT1 was downregulated in bone morphogenetic protein-7 (BMP-7)-induced cellular differentiation, while MALAT1 was upregulated in platelet-derived growth factor-BB (PDGF-BB)-induced cellular proliferation. PDGF induced the transformation of smooth muscle cells into a proliferative phenotype accompanied by an increase in autophagy. The downregulation of MALAT1 attenuated PDGF-BB-induced proliferation and migration by inhibiting autophagy. MALAT1 could act as a competing endogenous RNA (ceRNA) to regulate autophagy-related 7 (ATG7) gene expression by sponging miR142-3p. The present study reveals a novel mechanism by which MALAT1 promotes the transformation of smooth muscle cells from contraction to synthetic phenotypes.
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spelling pubmed-57969832018-02-12 LncRNA MALAT1 regulates smooth muscle cell phenotype switch via activation of autophagy Song, Tie-Feng Huang, Li-Wen Yuan, Ying Wang, Hui-qin He, Hong-Peng Ma, Wen-Jian Huo, Li-Hong Zhou, Hao Wang, Nan Zhang, Tong-Cun Oncotarget Research Paper Vascular smooth muscle cells (VSMCs), switching from a differentiated to a proliferative phenotype, contribute to various vascular diseases. However, the role of long noncoding RNA metastasis-associated lung adenocarcinoma transcript 1 MALAT1 in the phenotype switching of VSMCs remains unclear. Here, we report that the knockdown of MALAT1 promotes the transformation of smooth muscle cells from a proliferative phenotype to a differentiated phenotype. MALAT1 knockdown inhibited cellular proliferation and migration, leading to significant cell cycle arrest in the G2 phase. MALAT1 was downregulated in bone morphogenetic protein-7 (BMP-7)-induced cellular differentiation, while MALAT1 was upregulated in platelet-derived growth factor-BB (PDGF-BB)-induced cellular proliferation. PDGF induced the transformation of smooth muscle cells into a proliferative phenotype accompanied by an increase in autophagy. The downregulation of MALAT1 attenuated PDGF-BB-induced proliferation and migration by inhibiting autophagy. MALAT1 could act as a competing endogenous RNA (ceRNA) to regulate autophagy-related 7 (ATG7) gene expression by sponging miR142-3p. The present study reveals a novel mechanism by which MALAT1 promotes the transformation of smooth muscle cells from contraction to synthetic phenotypes. Impact Journals LLC 2017-12-14 /pmc/articles/PMC5796983/ /pubmed/29435112 http://dx.doi.org/10.18632/oncotarget.23230 Text en Copyright: © 2018 Song et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Research Paper
Song, Tie-Feng
Huang, Li-Wen
Yuan, Ying
Wang, Hui-qin
He, Hong-Peng
Ma, Wen-Jian
Huo, Li-Hong
Zhou, Hao
Wang, Nan
Zhang, Tong-Cun
LncRNA MALAT1 regulates smooth muscle cell phenotype switch via activation of autophagy
title LncRNA MALAT1 regulates smooth muscle cell phenotype switch via activation of autophagy
title_full LncRNA MALAT1 regulates smooth muscle cell phenotype switch via activation of autophagy
title_fullStr LncRNA MALAT1 regulates smooth muscle cell phenotype switch via activation of autophagy
title_full_unstemmed LncRNA MALAT1 regulates smooth muscle cell phenotype switch via activation of autophagy
title_short LncRNA MALAT1 regulates smooth muscle cell phenotype switch via activation of autophagy
title_sort lncrna malat1 regulates smooth muscle cell phenotype switch via activation of autophagy
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796983/
https://www.ncbi.nlm.nih.gov/pubmed/29435112
http://dx.doi.org/10.18632/oncotarget.23230
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