Contractile Skeletal Muscle Cells Cultured with a Conducting Soft Wire for Effective, Selective Stimulation

Contractile skeletal muscle cells were cultured so as to wrap around an electrode wire to enable their selective stimulation even when they were co-cultured with other electrically-excitable cells. Since the electrode wire was composed of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDO...

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Autores principales: Nagamine, Kuniaki, Sato, Hirotaka, Kai, Hiroyuki, Kaji, Hirokazu, Kanzaki, Makoto, Nishizawa, Matsuhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797109/
https://www.ncbi.nlm.nih.gov/pubmed/29396483
http://dx.doi.org/10.1038/s41598-018-20729-y
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author Nagamine, Kuniaki
Sato, Hirotaka
Kai, Hiroyuki
Kaji, Hirokazu
Kanzaki, Makoto
Nishizawa, Matsuhiko
author_facet Nagamine, Kuniaki
Sato, Hirotaka
Kai, Hiroyuki
Kaji, Hirokazu
Kanzaki, Makoto
Nishizawa, Matsuhiko
author_sort Nagamine, Kuniaki
collection PubMed
description Contractile skeletal muscle cells were cultured so as to wrap around an electrode wire to enable their selective stimulation even when they were co-cultured with other electrically-excitable cells. Since the electrode wire was composed of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and polyurethane (PU), which is soft and highly capacitive (~10 mF cm(−2)), non-faradaic electrical stimulation with charge/discharge currents could be applied to the surrounding cells without causing significant damage even for longer periods (more than a week). The advantage of this new culture system was demonstrated in the study of chemotactic interaction of monocytes and skeletal muscle cells via myokines.
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spelling pubmed-57971092018-02-12 Contractile Skeletal Muscle Cells Cultured with a Conducting Soft Wire for Effective, Selective Stimulation Nagamine, Kuniaki Sato, Hirotaka Kai, Hiroyuki Kaji, Hirokazu Kanzaki, Makoto Nishizawa, Matsuhiko Sci Rep Article Contractile skeletal muscle cells were cultured so as to wrap around an electrode wire to enable their selective stimulation even when they were co-cultured with other electrically-excitable cells. Since the electrode wire was composed of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and polyurethane (PU), which is soft and highly capacitive (~10 mF cm(−2)), non-faradaic electrical stimulation with charge/discharge currents could be applied to the surrounding cells without causing significant damage even for longer periods (more than a week). The advantage of this new culture system was demonstrated in the study of chemotactic interaction of monocytes and skeletal muscle cells via myokines. Nature Publishing Group UK 2018-02-02 /pmc/articles/PMC5797109/ /pubmed/29396483 http://dx.doi.org/10.1038/s41598-018-20729-y Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Nagamine, Kuniaki
Sato, Hirotaka
Kai, Hiroyuki
Kaji, Hirokazu
Kanzaki, Makoto
Nishizawa, Matsuhiko
Contractile Skeletal Muscle Cells Cultured with a Conducting Soft Wire for Effective, Selective Stimulation
title Contractile Skeletal Muscle Cells Cultured with a Conducting Soft Wire for Effective, Selective Stimulation
title_full Contractile Skeletal Muscle Cells Cultured with a Conducting Soft Wire for Effective, Selective Stimulation
title_fullStr Contractile Skeletal Muscle Cells Cultured with a Conducting Soft Wire for Effective, Selective Stimulation
title_full_unstemmed Contractile Skeletal Muscle Cells Cultured with a Conducting Soft Wire for Effective, Selective Stimulation
title_short Contractile Skeletal Muscle Cells Cultured with a Conducting Soft Wire for Effective, Selective Stimulation
title_sort contractile skeletal muscle cells cultured with a conducting soft wire for effective, selective stimulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797109/
https://www.ncbi.nlm.nih.gov/pubmed/29396483
http://dx.doi.org/10.1038/s41598-018-20729-y
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