Cargando…

Comparative phosphoproteomic analysis reveals signaling networks regulating monopolar and bipolar cytokinesis

The successful completion of cytokinesis requires the coordinated activities of diverse cellular components including membranes, cytoskeletal elements and chromosomes that together form partly redundant pathways, depending on the cell type. The biochemical analysis of this process is challenging due...

Descripción completa

Detalles Bibliográficos
Autores principales: Karayel, Özge, Şanal, Erdem, Giese, Sven H., Üretmen Kagıalı, Zeynep Cansu, Polat, Ayşe Nur, Hu, Chi-Kuo, Renard, Bernhard Y., Tuncbag, Nurcan, Özlü, Nurhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797227/
https://www.ncbi.nlm.nih.gov/pubmed/29396449
http://dx.doi.org/10.1038/s41598-018-20231-5
_version_ 1783297640403828736
author Karayel, Özge
Şanal, Erdem
Giese, Sven H.
Üretmen Kagıalı, Zeynep Cansu
Polat, Ayşe Nur
Hu, Chi-Kuo
Renard, Bernhard Y.
Tuncbag, Nurcan
Özlü, Nurhan
author_facet Karayel, Özge
Şanal, Erdem
Giese, Sven H.
Üretmen Kagıalı, Zeynep Cansu
Polat, Ayşe Nur
Hu, Chi-Kuo
Renard, Bernhard Y.
Tuncbag, Nurcan
Özlü, Nurhan
author_sort Karayel, Özge
collection PubMed
description The successful completion of cytokinesis requires the coordinated activities of diverse cellular components including membranes, cytoskeletal elements and chromosomes that together form partly redundant pathways, depending on the cell type. The biochemical analysis of this process is challenging due to its dynamic and rapid nature. Here, we systematically compared monopolar and bipolar cytokinesis and demonstrated that monopolar cytokinesis is a good surrogate for cytokinesis and it is a well-suited system for global biochemical analysis in mammalian cells. Based on this, we established a phosphoproteomic signature of cytokinesis. More than 10,000 phosphorylation sites were systematically monitored; around 800 of those were up-regulated during cytokinesis. Reconstructing the kinase-substrate interaction network revealed 31 potentially active kinases during cytokinesis. The kinase-substrate network connects proteins between cytoskeleton, membrane and cell cycle machinery. We also found consensus motifs of phosphorylation sites that can serve as biochemical markers specific to cytokinesis. Beyond the kinase-substrate network, our reconstructed signaling network suggests that combination of sumoylation and phosphorylation may regulate monopolar cytokinesis specific signaling pathways. Our analysis provides a systematic approach to the comparison of different cytokinesis types to reveal alternative ways and a global overview, in which conserved genes work together and organize chromatin and cytoplasm during cytokinesis.
format Online
Article
Text
id pubmed-5797227
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-57972272018-02-13 Comparative phosphoproteomic analysis reveals signaling networks regulating monopolar and bipolar cytokinesis Karayel, Özge Şanal, Erdem Giese, Sven H. Üretmen Kagıalı, Zeynep Cansu Polat, Ayşe Nur Hu, Chi-Kuo Renard, Bernhard Y. Tuncbag, Nurcan Özlü, Nurhan Sci Rep Article The successful completion of cytokinesis requires the coordinated activities of diverse cellular components including membranes, cytoskeletal elements and chromosomes that together form partly redundant pathways, depending on the cell type. The biochemical analysis of this process is challenging due to its dynamic and rapid nature. Here, we systematically compared monopolar and bipolar cytokinesis and demonstrated that monopolar cytokinesis is a good surrogate for cytokinesis and it is a well-suited system for global biochemical analysis in mammalian cells. Based on this, we established a phosphoproteomic signature of cytokinesis. More than 10,000 phosphorylation sites were systematically monitored; around 800 of those were up-regulated during cytokinesis. Reconstructing the kinase-substrate interaction network revealed 31 potentially active kinases during cytokinesis. The kinase-substrate network connects proteins between cytoskeleton, membrane and cell cycle machinery. We also found consensus motifs of phosphorylation sites that can serve as biochemical markers specific to cytokinesis. Beyond the kinase-substrate network, our reconstructed signaling network suggests that combination of sumoylation and phosphorylation may regulate monopolar cytokinesis specific signaling pathways. Our analysis provides a systematic approach to the comparison of different cytokinesis types to reveal alternative ways and a global overview, in which conserved genes work together and organize chromatin and cytoplasm during cytokinesis. Nature Publishing Group UK 2018-02-02 /pmc/articles/PMC5797227/ /pubmed/29396449 http://dx.doi.org/10.1038/s41598-018-20231-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Karayel, Özge
Şanal, Erdem
Giese, Sven H.
Üretmen Kagıalı, Zeynep Cansu
Polat, Ayşe Nur
Hu, Chi-Kuo
Renard, Bernhard Y.
Tuncbag, Nurcan
Özlü, Nurhan
Comparative phosphoproteomic analysis reveals signaling networks regulating monopolar and bipolar cytokinesis
title Comparative phosphoproteomic analysis reveals signaling networks regulating monopolar and bipolar cytokinesis
title_full Comparative phosphoproteomic analysis reveals signaling networks regulating monopolar and bipolar cytokinesis
title_fullStr Comparative phosphoproteomic analysis reveals signaling networks regulating monopolar and bipolar cytokinesis
title_full_unstemmed Comparative phosphoproteomic analysis reveals signaling networks regulating monopolar and bipolar cytokinesis
title_short Comparative phosphoproteomic analysis reveals signaling networks regulating monopolar and bipolar cytokinesis
title_sort comparative phosphoproteomic analysis reveals signaling networks regulating monopolar and bipolar cytokinesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797227/
https://www.ncbi.nlm.nih.gov/pubmed/29396449
http://dx.doi.org/10.1038/s41598-018-20231-5
work_keys_str_mv AT karayelozge comparativephosphoproteomicanalysisrevealssignalingnetworksregulatingmonopolarandbipolarcytokinesis
AT sanalerdem comparativephosphoproteomicanalysisrevealssignalingnetworksregulatingmonopolarandbipolarcytokinesis
AT giesesvenh comparativephosphoproteomicanalysisrevealssignalingnetworksregulatingmonopolarandbipolarcytokinesis
AT uretmenkagıalızeynepcansu comparativephosphoproteomicanalysisrevealssignalingnetworksregulatingmonopolarandbipolarcytokinesis
AT polataysenur comparativephosphoproteomicanalysisrevealssignalingnetworksregulatingmonopolarandbipolarcytokinesis
AT huchikuo comparativephosphoproteomicanalysisrevealssignalingnetworksregulatingmonopolarandbipolarcytokinesis
AT renardbernhardy comparativephosphoproteomicanalysisrevealssignalingnetworksregulatingmonopolarandbipolarcytokinesis
AT tuncbagnurcan comparativephosphoproteomicanalysisrevealssignalingnetworksregulatingmonopolarandbipolarcytokinesis
AT ozlunurhan comparativephosphoproteomicanalysisrevealssignalingnetworksregulatingmonopolarandbipolarcytokinesis