The Effect of DNA Extraction Methods on Observed Microbial Communities from Fibrous and Liquid Rumen Fractions of Dairy Cows

DNA based methods have been widely used to study the complexity of the rumen microbiota, and it is well known that the method of DNA extraction is a critical step in enabling accurate assessment of this complexity. Rumen fluid (RF) and fibrous content (FC) fractions differ substantially in terms of...

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Detalles Bibliográficos
Autores principales: Vaidya, Jueeli D., van den Bogert, Bartholomeus, Edwards, Joan E., Boekhorst, Jos, van Gastelen, Sanne, Saccenti, Edoardo, Plugge, Caroline M., Smidt, Hauke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797766/
https://www.ncbi.nlm.nih.gov/pubmed/29445366
http://dx.doi.org/10.3389/fmicb.2018.00092

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