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Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection

Eosinophils are multifunctional cells that have cytotoxic proinflammatory activities and stimulate CD4(+) T-cells in experimental models of allergy and parasitic infections. Eosinophils, when exposed to antigens, are activated, expressing the CD38/CD69 molecules and exhibited increased expression of...

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Autores principales: Rodolpho, Joice Margareth de Almeida, Camillo, Luciana, Araújo, Márcio Sobreira Silva, Speziali, Elaine, Coelho-dos-Reis, Jordana Grazziela, Correia, Ricardo de Oliveira, Neris, Débora Meira, Martins-Filho, Olindo Assis, Teixeira-Carvalho, Andréa, Anibal, Fernanda de Freitas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797789/
https://www.ncbi.nlm.nih.gov/pubmed/29445372
http://dx.doi.org/10.3389/fimmu.2018.00064
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author Rodolpho, Joice Margareth de Almeida
Camillo, Luciana
Araújo, Márcio Sobreira Silva
Speziali, Elaine
Coelho-dos-Reis, Jordana Grazziela
Correia, Ricardo de Oliveira
Neris, Débora Meira
Martins-Filho, Olindo Assis
Teixeira-Carvalho, Andréa
Anibal, Fernanda de Freitas
author_facet Rodolpho, Joice Margareth de Almeida
Camillo, Luciana
Araújo, Márcio Sobreira Silva
Speziali, Elaine
Coelho-dos-Reis, Jordana Grazziela
Correia, Ricardo de Oliveira
Neris, Débora Meira
Martins-Filho, Olindo Assis
Teixeira-Carvalho, Andréa
Anibal, Fernanda de Freitas
author_sort Rodolpho, Joice Margareth de Almeida
collection PubMed
description Eosinophils are multifunctional cells that have cytotoxic proinflammatory activities and stimulate CD4(+) T-cells in experimental models of allergy and parasitic infections. Eosinophils, when exposed to antigens, are activated, expressing the CD38/CD69 molecules and exhibited increased expression of major histocompatibility complex (MHC-II), CD80 and CD86, suggesting they play a role upon Toxocara canis antigen stimulation. In the present study, we evaluated the profile of eosinophils using conventional and image flow cytometry upon experimental T. canis infection. T. canis antigens induced a robust activation on this subset, contributing to the immune responses elicited in the experimental model for T. canis-associated visceral larva migrans syndrome. Data analysis demonstrated that, during murine T. canis infection, eosinophils from peripheral blood, spleen, and bone marrow presented upregulated expression of CD69/MHC-II/CD80/CD86. As opposed to splenic and bone marrow eosinophils, circulating eosinophils had increased expression of activation markers upon T. canis infection. The enhanced connectivity between eosinophils and T-cells in T. canis-infected mice in all three compartments (peripheral blood, spleen, and bone marrow) also supports the hypothesis that eosinophils may adopt a role during T. canis infection. Moreover, in vitro T. canis antigen stimulation resulted in activation and upregulation of co-stimulatory-related molecules by bone marrow-derived eosinophils. Our findings are evidence of activation and upregulation of important activation and co-stimulatory-related molecules in eosinophils and suggest a reshape of activation hierarchy toward eosinophils during experimental T. canis infection.
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spelling pubmed-57977892018-02-14 Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection Rodolpho, Joice Margareth de Almeida Camillo, Luciana Araújo, Márcio Sobreira Silva Speziali, Elaine Coelho-dos-Reis, Jordana Grazziela Correia, Ricardo de Oliveira Neris, Débora Meira Martins-Filho, Olindo Assis Teixeira-Carvalho, Andréa Anibal, Fernanda de Freitas Front Immunol Immunology Eosinophils are multifunctional cells that have cytotoxic proinflammatory activities and stimulate CD4(+) T-cells in experimental models of allergy and parasitic infections. Eosinophils, when exposed to antigens, are activated, expressing the CD38/CD69 molecules and exhibited increased expression of major histocompatibility complex (MHC-II), CD80 and CD86, suggesting they play a role upon Toxocara canis antigen stimulation. In the present study, we evaluated the profile of eosinophils using conventional and image flow cytometry upon experimental T. canis infection. T. canis antigens induced a robust activation on this subset, contributing to the immune responses elicited in the experimental model for T. canis-associated visceral larva migrans syndrome. Data analysis demonstrated that, during murine T. canis infection, eosinophils from peripheral blood, spleen, and bone marrow presented upregulated expression of CD69/MHC-II/CD80/CD86. As opposed to splenic and bone marrow eosinophils, circulating eosinophils had increased expression of activation markers upon T. canis infection. The enhanced connectivity between eosinophils and T-cells in T. canis-infected mice in all three compartments (peripheral blood, spleen, and bone marrow) also supports the hypothesis that eosinophils may adopt a role during T. canis infection. Moreover, in vitro T. canis antigen stimulation resulted in activation and upregulation of co-stimulatory-related molecules by bone marrow-derived eosinophils. Our findings are evidence of activation and upregulation of important activation and co-stimulatory-related molecules in eosinophils and suggest a reshape of activation hierarchy toward eosinophils during experimental T. canis infection. Frontiers Media S.A. 2018-01-31 /pmc/articles/PMC5797789/ /pubmed/29445372 http://dx.doi.org/10.3389/fimmu.2018.00064 Text en Copyright © 2018 Rodolpho, Camillo, Araújo, Speziali, Coelho-dos-Reis, Correia, Neris, Martins-Filho, Teixeira-Carvalho and Anibal. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Rodolpho, Joice Margareth de Almeida
Camillo, Luciana
Araújo, Márcio Sobreira Silva
Speziali, Elaine
Coelho-dos-Reis, Jordana Grazziela
Correia, Ricardo de Oliveira
Neris, Débora Meira
Martins-Filho, Olindo Assis
Teixeira-Carvalho, Andréa
Anibal, Fernanda de Freitas
Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
title Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
title_full Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
title_fullStr Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
title_full_unstemmed Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
title_short Robust Phenotypic Activation of Eosinophils during Experimental Toxocara canis Infection
title_sort robust phenotypic activation of eosinophils during experimental toxocara canis infection
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797789/
https://www.ncbi.nlm.nih.gov/pubmed/29445372
http://dx.doi.org/10.3389/fimmu.2018.00064
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