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Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia
Rodents have historically been associated with zoonotic pandemics that claimed the lives of large human populations. Appropriate pathogen surveillance initiatives could contribute to early detection of zoonotic infections to prevent future outbreaks. Bordetella species are bacteria known to cause mi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Japanese Society of Veterinary Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797863/ https://www.ncbi.nlm.nih.gov/pubmed/29237995 http://dx.doi.org/10.1292/jvms.17-0218 |
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author | LOONG, Shih Keng CHE-MAT-SERI, Nurul-Asma-Anati ABDULRAZAK, Osama DOUADI, Benacer AHMAD-NASRAH, Siti-Noraisah JOHARI, Jefree MOHD-ZAIN, Siti-Nursheena ABUBAKAR, Sazaly |
author_facet | LOONG, Shih Keng CHE-MAT-SERI, Nurul-Asma-Anati ABDULRAZAK, Osama DOUADI, Benacer AHMAD-NASRAH, Siti-Noraisah JOHARI, Jefree MOHD-ZAIN, Siti-Nursheena ABUBAKAR, Sazaly |
author_sort | LOONG, Shih Keng |
collection | PubMed |
description | Rodents have historically been associated with zoonotic pandemics that claimed the lives of large human populations. Appropriate pathogen surveillance initiatives could contribute to early detection of zoonotic infections to prevent future outbreaks. Bordetella species are bacteria known to cause mild to severe respiratory disease in mammals and, some have been described to infect, colonize and spread in rodents. There is a lack of information on the population diversity of bordetellae among Malaysian wild rodents. Here, bordetellae recovered from lung tissues of wild rats were genotypically characterized using 16S rDNA sequencing, MLST and nrdA typing. A novel B. bronchiseptica ST82, closely related to other human-derived isolates, was discovered in three wild rats (n=3) from Terengganu (5.3333° N, 103.1500° E). B. pseudohinzii, a recently identified laboratory mice inhabitant, was also recovered from one rat (n=1). Both bordetellae displayed identical antimicrobial resistance profiles, indicating the close phylogenetic association between them. Genotyping using the 765-bp nrdA locus was shown to be compatible with the MLST-based phylogeny, with the added advantage of being able to genotype non-classical bordetellae. The recovery of B. pseudohinzii from wild rat implied that this bordetellae has a wider host range than previously thought. The findings from this study suggest that bordetellae surveillance among wild rats in Malaysia has to be continued and expanded to other states to ensure early identification of species capable of causing public health disorder. |
format | Online Article Text |
id | pubmed-5797863 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-57978632018-02-08 Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia LOONG, Shih Keng CHE-MAT-SERI, Nurul-Asma-Anati ABDULRAZAK, Osama DOUADI, Benacer AHMAD-NASRAH, Siti-Noraisah JOHARI, Jefree MOHD-ZAIN, Siti-Nursheena ABUBAKAR, Sazaly J Vet Med Sci Bacteriology Rodents have historically been associated with zoonotic pandemics that claimed the lives of large human populations. Appropriate pathogen surveillance initiatives could contribute to early detection of zoonotic infections to prevent future outbreaks. Bordetella species are bacteria known to cause mild to severe respiratory disease in mammals and, some have been described to infect, colonize and spread in rodents. There is a lack of information on the population diversity of bordetellae among Malaysian wild rodents. Here, bordetellae recovered from lung tissues of wild rats were genotypically characterized using 16S rDNA sequencing, MLST and nrdA typing. A novel B. bronchiseptica ST82, closely related to other human-derived isolates, was discovered in three wild rats (n=3) from Terengganu (5.3333° N, 103.1500° E). B. pseudohinzii, a recently identified laboratory mice inhabitant, was also recovered from one rat (n=1). Both bordetellae displayed identical antimicrobial resistance profiles, indicating the close phylogenetic association between them. Genotyping using the 765-bp nrdA locus was shown to be compatible with the MLST-based phylogeny, with the added advantage of being able to genotype non-classical bordetellae. The recovery of B. pseudohinzii from wild rat implied that this bordetellae has a wider host range than previously thought. The findings from this study suggest that bordetellae surveillance among wild rats in Malaysia has to be continued and expanded to other states to ensure early identification of species capable of causing public health disorder. The Japanese Society of Veterinary Science 2017-12-13 2018-01 /pmc/articles/PMC5797863/ /pubmed/29237995 http://dx.doi.org/10.1292/jvms.17-0218 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Bacteriology LOONG, Shih Keng CHE-MAT-SERI, Nurul-Asma-Anati ABDULRAZAK, Osama DOUADI, Benacer AHMAD-NASRAH, Siti-Noraisah JOHARI, Jefree MOHD-ZAIN, Siti-Nursheena ABUBAKAR, Sazaly Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia |
title | Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia |
title_full | Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia |
title_fullStr | Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia |
title_full_unstemmed | Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia |
title_short | Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia |
title_sort | recovery of bordetella bronchiseptica sequence type 82 and b. pseudohinzii from urban rats in terengganu, malaysia |
topic | Bacteriology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797863/ https://www.ncbi.nlm.nih.gov/pubmed/29237995 http://dx.doi.org/10.1292/jvms.17-0218 |
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