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Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia

Rodents have historically been associated with zoonotic pandemics that claimed the lives of large human populations. Appropriate pathogen surveillance initiatives could contribute to early detection of zoonotic infections to prevent future outbreaks. Bordetella species are bacteria known to cause mi...

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Autores principales: LOONG, Shih Keng, CHE-MAT-SERI, Nurul-Asma-Anati, ABDULRAZAK, Osama, DOUADI, Benacer, AHMAD-NASRAH, Siti-Noraisah, JOHARI, Jefree, MOHD-ZAIN, Siti-Nursheena, ABUBAKAR, Sazaly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797863/
https://www.ncbi.nlm.nih.gov/pubmed/29237995
http://dx.doi.org/10.1292/jvms.17-0218
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author LOONG, Shih Keng
CHE-MAT-SERI, Nurul-Asma-Anati
ABDULRAZAK, Osama
DOUADI, Benacer
AHMAD-NASRAH, Siti-Noraisah
JOHARI, Jefree
MOHD-ZAIN, Siti-Nursheena
ABUBAKAR, Sazaly
author_facet LOONG, Shih Keng
CHE-MAT-SERI, Nurul-Asma-Anati
ABDULRAZAK, Osama
DOUADI, Benacer
AHMAD-NASRAH, Siti-Noraisah
JOHARI, Jefree
MOHD-ZAIN, Siti-Nursheena
ABUBAKAR, Sazaly
author_sort LOONG, Shih Keng
collection PubMed
description Rodents have historically been associated with zoonotic pandemics that claimed the lives of large human populations. Appropriate pathogen surveillance initiatives could contribute to early detection of zoonotic infections to prevent future outbreaks. Bordetella species are bacteria known to cause mild to severe respiratory disease in mammals and, some have been described to infect, colonize and spread in rodents. There is a lack of information on the population diversity of bordetellae among Malaysian wild rodents. Here, bordetellae recovered from lung tissues of wild rats were genotypically characterized using 16S rDNA sequencing, MLST and nrdA typing. A novel B. bronchiseptica ST82, closely related to other human-derived isolates, was discovered in three wild rats (n=3) from Terengganu (5.3333° N, 103.1500° E). B. pseudohinzii, a recently identified laboratory mice inhabitant, was also recovered from one rat (n=1). Both bordetellae displayed identical antimicrobial resistance profiles, indicating the close phylogenetic association between them. Genotyping using the 765-bp nrdA locus was shown to be compatible with the MLST-based phylogeny, with the added advantage of being able to genotype non-classical bordetellae. The recovery of B. pseudohinzii from wild rat implied that this bordetellae has a wider host range than previously thought. The findings from this study suggest that bordetellae surveillance among wild rats in Malaysia has to be continued and expanded to other states to ensure early identification of species capable of causing public health disorder.
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spelling pubmed-57978632018-02-08 Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia LOONG, Shih Keng CHE-MAT-SERI, Nurul-Asma-Anati ABDULRAZAK, Osama DOUADI, Benacer AHMAD-NASRAH, Siti-Noraisah JOHARI, Jefree MOHD-ZAIN, Siti-Nursheena ABUBAKAR, Sazaly J Vet Med Sci Bacteriology Rodents have historically been associated with zoonotic pandemics that claimed the lives of large human populations. Appropriate pathogen surveillance initiatives could contribute to early detection of zoonotic infections to prevent future outbreaks. Bordetella species are bacteria known to cause mild to severe respiratory disease in mammals and, some have been described to infect, colonize and spread in rodents. There is a lack of information on the population diversity of bordetellae among Malaysian wild rodents. Here, bordetellae recovered from lung tissues of wild rats were genotypically characterized using 16S rDNA sequencing, MLST and nrdA typing. A novel B. bronchiseptica ST82, closely related to other human-derived isolates, was discovered in three wild rats (n=3) from Terengganu (5.3333° N, 103.1500° E). B. pseudohinzii, a recently identified laboratory mice inhabitant, was also recovered from one rat (n=1). Both bordetellae displayed identical antimicrobial resistance profiles, indicating the close phylogenetic association between them. Genotyping using the 765-bp nrdA locus was shown to be compatible with the MLST-based phylogeny, with the added advantage of being able to genotype non-classical bordetellae. The recovery of B. pseudohinzii from wild rat implied that this bordetellae has a wider host range than previously thought. The findings from this study suggest that bordetellae surveillance among wild rats in Malaysia has to be continued and expanded to other states to ensure early identification of species capable of causing public health disorder. The Japanese Society of Veterinary Science 2017-12-13 2018-01 /pmc/articles/PMC5797863/ /pubmed/29237995 http://dx.doi.org/10.1292/jvms.17-0218 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Bacteriology
LOONG, Shih Keng
CHE-MAT-SERI, Nurul-Asma-Anati
ABDULRAZAK, Osama
DOUADI, Benacer
AHMAD-NASRAH, Siti-Noraisah
JOHARI, Jefree
MOHD-ZAIN, Siti-Nursheena
ABUBAKAR, Sazaly
Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia
title Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia
title_full Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia
title_fullStr Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia
title_full_unstemmed Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia
title_short Recovery of Bordetella bronchiseptica sequence type 82 and B. pseudohinzii from urban rats in Terengganu, Malaysia
title_sort recovery of bordetella bronchiseptica sequence type 82 and b. pseudohinzii from urban rats in terengganu, malaysia
topic Bacteriology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797863/
https://www.ncbi.nlm.nih.gov/pubmed/29237995
http://dx.doi.org/10.1292/jvms.17-0218
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