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Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss

The mitochondrial genome of moss Mielichhoferia elongata has been sequenced and assembled with Spades genome assembler. It consists of 100,342 base pairs and has practically the same gene set and order as in other known bryophyte chondriomes. The genome contains 66 genes including three rRNAs, 24 tR...

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Autores principales: Goruynov, Denis V., Goryunova, Svetlana V., Kuznetsova, Oxana I., Logacheva, Maria D., Milyutina, Irina A., Fedorova, Alina V., Ignatov, Michael S., Troitsky, Aleksey V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5798402/
https://www.ncbi.nlm.nih.gov/pubmed/29416956
http://dx.doi.org/10.7717/peerj.4350
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author Goruynov, Denis V.
Goryunova, Svetlana V.
Kuznetsova, Oxana I.
Logacheva, Maria D.
Milyutina, Irina A.
Fedorova, Alina V.
Ignatov, Michael S.
Troitsky, Aleksey V.
author_facet Goruynov, Denis V.
Goryunova, Svetlana V.
Kuznetsova, Oxana I.
Logacheva, Maria D.
Milyutina, Irina A.
Fedorova, Alina V.
Ignatov, Michael S.
Troitsky, Aleksey V.
author_sort Goruynov, Denis V.
collection PubMed
description The mitochondrial genome of moss Mielichhoferia elongata has been sequenced and assembled with Spades genome assembler. It consists of 100,342 base pairs and has practically the same gene set and order as in other known bryophyte chondriomes. The genome contains 66 genes including three rRNAs, 24 tRNAs, and 40 conserved mitochondrial proteins genes. Unlike the majority of previously sequenced bryophyte mitogenomes, it lacks the functional nad7 gene. The phylogenetic reconstruction and scrutiny analysis of the primary structure of nad7 gene carried out in this study suggest its independent pseudogenization in different bryophyte lineages. Evaluation of the microsatellite (simple sequence repeat) content of the M. elongata mitochondrial genome indicates that it could be used as a tool in further studies as a phylogenetic marker. The strongly supported phylogenetic tree presented here, derived from 33 protein coding sequences of 40 bryophyte species, is consistent with other reconstructions based on a number of different data sets.
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spelling pubmed-57984022018-02-07 Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss Goruynov, Denis V. Goryunova, Svetlana V. Kuznetsova, Oxana I. Logacheva, Maria D. Milyutina, Irina A. Fedorova, Alina V. Ignatov, Michael S. Troitsky, Aleksey V. PeerJ Biodiversity The mitochondrial genome of moss Mielichhoferia elongata has been sequenced and assembled with Spades genome assembler. It consists of 100,342 base pairs and has practically the same gene set and order as in other known bryophyte chondriomes. The genome contains 66 genes including three rRNAs, 24 tRNAs, and 40 conserved mitochondrial proteins genes. Unlike the majority of previously sequenced bryophyte mitogenomes, it lacks the functional nad7 gene. The phylogenetic reconstruction and scrutiny analysis of the primary structure of nad7 gene carried out in this study suggest its independent pseudogenization in different bryophyte lineages. Evaluation of the microsatellite (simple sequence repeat) content of the M. elongata mitochondrial genome indicates that it could be used as a tool in further studies as a phylogenetic marker. The strongly supported phylogenetic tree presented here, derived from 33 protein coding sequences of 40 bryophyte species, is consistent with other reconstructions based on a number of different data sets. PeerJ Inc. 2018-02-02 /pmc/articles/PMC5798402/ /pubmed/29416956 http://dx.doi.org/10.7717/peerj.4350 Text en © 2018 Goruynov et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biodiversity
Goruynov, Denis V.
Goryunova, Svetlana V.
Kuznetsova, Oxana I.
Logacheva, Maria D.
Milyutina, Irina A.
Fedorova, Alina V.
Ignatov, Michael S.
Troitsky, Aleksey V.
Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss
title Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss
title_full Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss
title_fullStr Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss
title_full_unstemmed Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss
title_short Complete mitochondrial genome sequence of the “copper moss” Mielichhoferia elongata reveals independent nad7 gene functionality loss
title_sort complete mitochondrial genome sequence of the “copper moss” mielichhoferia elongata reveals independent nad7 gene functionality loss
topic Biodiversity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5798402/
https://www.ncbi.nlm.nih.gov/pubmed/29416956
http://dx.doi.org/10.7717/peerj.4350
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