Clinical value of ALU concentration and integrity index for the early diagnosis of ovarian cancer: A retrospective cohort trial

CA125 amounts have a large overlap in ovarian cancer and benign diseases. We conducted a retrospective cohort trial to assess the clinical value of circulating cell-free DNA concentration and integrity index for the diagnosis of ovarian cancer. A total of 150 patients were recruited. Plasma samples...

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Autores principales: Zhang, Rong, PU, Wangyang, Zhang, Shuyun, Chen, Li, Zhu, Weipei, Xiao, Li, Xing, Chungen, Li, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5798773/
https://www.ncbi.nlm.nih.gov/pubmed/29401471
http://dx.doi.org/10.1371/journal.pone.0191756
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author Zhang, Rong
PU, Wangyang
Zhang, Shuyun
Chen, Li
Zhu, Weipei
Xiao, Li
Xing, Chungen
Li, Kai
author_facet Zhang, Rong
PU, Wangyang
Zhang, Shuyun
Chen, Li
Zhu, Weipei
Xiao, Li
Xing, Chungen
Li, Kai
author_sort Zhang, Rong
collection PubMed
description CA125 amounts have a large overlap in ovarian cancer and benign diseases. We conducted a retrospective cohort trial to assess the clinical value of circulating cell-free DNA concentration and integrity index for the diagnosis of ovarian cancer. A total of 150 patients were recruited. Plasma samples of 24 ovarian cancer patients, 12 benign ovarian cysts, and 12 healthy controls were assessed. By amplifying short ALU-115 repeat and long ALU-219 fragments, circulating cell-free DNA concentrations and integrity index were measured. Plasma ALU-219 fragment levels and integrity index were significantly higher in the ovarian cancer group compared with the benign disease and healthy control groups (p = 0.023 and p = 0.004, respectively). These findings indicated that plasma ALU-219 levels and integrity may have a clinical value in the early diagnosis of ovarian cancer.
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spelling pubmed-57987732018-02-23 Clinical value of ALU concentration and integrity index for the early diagnosis of ovarian cancer: A retrospective cohort trial Zhang, Rong PU, Wangyang Zhang, Shuyun Chen, Li Zhu, Weipei Xiao, Li Xing, Chungen Li, Kai PLoS One Research Article CA125 amounts have a large overlap in ovarian cancer and benign diseases. We conducted a retrospective cohort trial to assess the clinical value of circulating cell-free DNA concentration and integrity index for the diagnosis of ovarian cancer. A total of 150 patients were recruited. Plasma samples of 24 ovarian cancer patients, 12 benign ovarian cysts, and 12 healthy controls were assessed. By amplifying short ALU-115 repeat and long ALU-219 fragments, circulating cell-free DNA concentrations and integrity index were measured. Plasma ALU-219 fragment levels and integrity index were significantly higher in the ovarian cancer group compared with the benign disease and healthy control groups (p = 0.023 and p = 0.004, respectively). These findings indicated that plasma ALU-219 levels and integrity may have a clinical value in the early diagnosis of ovarian cancer. Public Library of Science 2018-02-05 /pmc/articles/PMC5798773/ /pubmed/29401471 http://dx.doi.org/10.1371/journal.pone.0191756 Text en © 2018 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhang, Rong
PU, Wangyang
Zhang, Shuyun
Chen, Li
Zhu, Weipei
Xiao, Li
Xing, Chungen
Li, Kai
Clinical value of ALU concentration and integrity index for the early diagnosis of ovarian cancer: A retrospective cohort trial
title Clinical value of ALU concentration and integrity index for the early diagnosis of ovarian cancer: A retrospective cohort trial
title_full Clinical value of ALU concentration and integrity index for the early diagnosis of ovarian cancer: A retrospective cohort trial
title_fullStr Clinical value of ALU concentration and integrity index for the early diagnosis of ovarian cancer: A retrospective cohort trial
title_full_unstemmed Clinical value of ALU concentration and integrity index for the early diagnosis of ovarian cancer: A retrospective cohort trial
title_short Clinical value of ALU concentration and integrity index for the early diagnosis of ovarian cancer: A retrospective cohort trial
title_sort clinical value of alu concentration and integrity index for the early diagnosis of ovarian cancer: a retrospective cohort trial
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5798773/
https://www.ncbi.nlm.nih.gov/pubmed/29401471
http://dx.doi.org/10.1371/journal.pone.0191756
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