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A comprehensive profile of circulating RNAs in human serum

Non-coding RNA (ncRNA) molecules have fundamental roles in cells and many are also stable in body fluids as extracellular RNAs. In this study, we used RNA sequencing (RNA-seq) to investigate the profile of small non-coding RNA (sncRNA) in human serum. We analyzed 10 billion Illumina reads from 477 s...

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Autores principales: Umu, Sinan Uğur, Langseth, Hilde, Bucher-Johannessen, Cecilie, Fromm, Bastian, Keller, Andreas, Meese, Eckart, Lauritzen, Marianne, Leithaug, Magnus, Lyle, Robert, Rounge, Trine B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5798962/
https://www.ncbi.nlm.nih.gov/pubmed/29219730
http://dx.doi.org/10.1080/15476286.2017.1403003
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author Umu, Sinan Uğur
Langseth, Hilde
Bucher-Johannessen, Cecilie
Fromm, Bastian
Keller, Andreas
Meese, Eckart
Lauritzen, Marianne
Leithaug, Magnus
Lyle, Robert
Rounge, Trine B.
author_facet Umu, Sinan Uğur
Langseth, Hilde
Bucher-Johannessen, Cecilie
Fromm, Bastian
Keller, Andreas
Meese, Eckart
Lauritzen, Marianne
Leithaug, Magnus
Lyle, Robert
Rounge, Trine B.
author_sort Umu, Sinan Uğur
collection PubMed
description Non-coding RNA (ncRNA) molecules have fundamental roles in cells and many are also stable in body fluids as extracellular RNAs. In this study, we used RNA sequencing (RNA-seq) to investigate the profile of small non-coding RNA (sncRNA) in human serum. We analyzed 10 billion Illumina reads from 477 serum samples, included in the Norwegian population-based Janus Serum Bank (JSB). We found that the core serum RNA repertoire includes 258 micro RNAs (miRNA), 441 piwi-interacting RNAs (piRNA), 411 transfer RNAs (tRNA), 24 small nucleolar RNAs (snoRNA), 125 small nuclear RNAs (snRNA) and 123 miscellaneous RNAs (misc-RNA). We also investigated biological and technical variation in expression, and the results suggest that many RNA molecules identified in serum contain signs of biological variation. They are therefore unlikely to be random degradation by-products. In addition, the presence of specific fragments of tRNA, snoRNA, Vault RNA and Y_RNA indicates protection from degradation. Our results suggest that many circulating RNAs in serum can be potential biomarkers.
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spelling pubmed-57989622018-02-07 A comprehensive profile of circulating RNAs in human serum Umu, Sinan Uğur Langseth, Hilde Bucher-Johannessen, Cecilie Fromm, Bastian Keller, Andreas Meese, Eckart Lauritzen, Marianne Leithaug, Magnus Lyle, Robert Rounge, Trine B. RNA Biol Research Papers Non-coding RNA (ncRNA) molecules have fundamental roles in cells and many are also stable in body fluids as extracellular RNAs. In this study, we used RNA sequencing (RNA-seq) to investigate the profile of small non-coding RNA (sncRNA) in human serum. We analyzed 10 billion Illumina reads from 477 serum samples, included in the Norwegian population-based Janus Serum Bank (JSB). We found that the core serum RNA repertoire includes 258 micro RNAs (miRNA), 441 piwi-interacting RNAs (piRNA), 411 transfer RNAs (tRNA), 24 small nucleolar RNAs (snoRNA), 125 small nuclear RNAs (snRNA) and 123 miscellaneous RNAs (misc-RNA). We also investigated biological and technical variation in expression, and the results suggest that many RNA molecules identified in serum contain signs of biological variation. They are therefore unlikely to be random degradation by-products. In addition, the presence of specific fragments of tRNA, snoRNA, Vault RNA and Y_RNA indicates protection from degradation. Our results suggest that many circulating RNAs in serum can be potential biomarkers. Taylor & Francis 2017-12-08 /pmc/articles/PMC5798962/ /pubmed/29219730 http://dx.doi.org/10.1080/15476286.2017.1403003 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Research Papers
Umu, Sinan Uğur
Langseth, Hilde
Bucher-Johannessen, Cecilie
Fromm, Bastian
Keller, Andreas
Meese, Eckart
Lauritzen, Marianne
Leithaug, Magnus
Lyle, Robert
Rounge, Trine B.
A comprehensive profile of circulating RNAs in human serum
title A comprehensive profile of circulating RNAs in human serum
title_full A comprehensive profile of circulating RNAs in human serum
title_fullStr A comprehensive profile of circulating RNAs in human serum
title_full_unstemmed A comprehensive profile of circulating RNAs in human serum
title_short A comprehensive profile of circulating RNAs in human serum
title_sort comprehensive profile of circulating rnas in human serum
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5798962/
https://www.ncbi.nlm.nih.gov/pubmed/29219730
http://dx.doi.org/10.1080/15476286.2017.1403003
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