Cargando…
Neuroprotection of the hypoxic-ischemic mouse brain by human CD117(+)CD90(+)CD105(+) amniotic fluid stem cells
Human amniotic fluid contains two morphologically-distinct sub-populations of stem cells with regenerative potential, spindle-shaped (SS-hAFSCs) and round-shaped human amniotic fluid stem cells (RS-hAFSCs). However, it is unclear whether morphological differences correlate with functionality, and th...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799160/ https://www.ncbi.nlm.nih.gov/pubmed/29402914 http://dx.doi.org/10.1038/s41598-018-20710-9 |
_version_ | 1783297935453192192 |
---|---|
author | Corcelli, Michelangelo Hawkins, Kate Vlahova, Filipa Hunjan, Avina Dowding, Kate De Coppi, Paolo David, Anna L. Peebles, Donald Gressens, Pierre Hagberg, Henrik Hristova, Mariya Guillot, Pascale V. |
author_facet | Corcelli, Michelangelo Hawkins, Kate Vlahova, Filipa Hunjan, Avina Dowding, Kate De Coppi, Paolo David, Anna L. Peebles, Donald Gressens, Pierre Hagberg, Henrik Hristova, Mariya Guillot, Pascale V. |
author_sort | Corcelli, Michelangelo |
collection | PubMed |
description | Human amniotic fluid contains two morphologically-distinct sub-populations of stem cells with regenerative potential, spindle-shaped (SS-hAFSCs) and round-shaped human amniotic fluid stem cells (RS-hAFSCs). However, it is unclear whether morphological differences correlate with functionality, and this lack of knowledge limits their translational applications. Here, we show that SS-hAFSCs and RS-hAFSCs differ in their neuro-protective ability, demonstrating that a single contralateral injection of SS-hAFSCs into hypoxic-ischemic P7 mice conferred a 47% reduction in hippocampal tissue loss and 43–45% reduction in TUNEL-positive cells in the hippocampus and striatum 48 hours after the insult, decreased microglial activation and TGFβ1 levels, and prevented demyelination. On the other hand, RS-hAFSCs failed to show such neuro-protective effects. It is possible that SS-hAFSCs exert their neuroprotection via endoglin-dependent inhibition of TGFβ1 signaling in target cells. These findings identify a sub-population of CD117(+)CD90(+)CD105(+) stem cells as a promising source for the neuro-protection of the developing brain. |
format | Online Article Text |
id | pubmed-5799160 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57991602018-02-14 Neuroprotection of the hypoxic-ischemic mouse brain by human CD117(+)CD90(+)CD105(+) amniotic fluid stem cells Corcelli, Michelangelo Hawkins, Kate Vlahova, Filipa Hunjan, Avina Dowding, Kate De Coppi, Paolo David, Anna L. Peebles, Donald Gressens, Pierre Hagberg, Henrik Hristova, Mariya Guillot, Pascale V. Sci Rep Article Human amniotic fluid contains two morphologically-distinct sub-populations of stem cells with regenerative potential, spindle-shaped (SS-hAFSCs) and round-shaped human amniotic fluid stem cells (RS-hAFSCs). However, it is unclear whether morphological differences correlate with functionality, and this lack of knowledge limits their translational applications. Here, we show that SS-hAFSCs and RS-hAFSCs differ in their neuro-protective ability, demonstrating that a single contralateral injection of SS-hAFSCs into hypoxic-ischemic P7 mice conferred a 47% reduction in hippocampal tissue loss and 43–45% reduction in TUNEL-positive cells in the hippocampus and striatum 48 hours after the insult, decreased microglial activation and TGFβ1 levels, and prevented demyelination. On the other hand, RS-hAFSCs failed to show such neuro-protective effects. It is possible that SS-hAFSCs exert their neuroprotection via endoglin-dependent inhibition of TGFβ1 signaling in target cells. These findings identify a sub-population of CD117(+)CD90(+)CD105(+) stem cells as a promising source for the neuro-protection of the developing brain. Nature Publishing Group UK 2018-02-05 /pmc/articles/PMC5799160/ /pubmed/29402914 http://dx.doi.org/10.1038/s41598-018-20710-9 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Corcelli, Michelangelo Hawkins, Kate Vlahova, Filipa Hunjan, Avina Dowding, Kate De Coppi, Paolo David, Anna L. Peebles, Donald Gressens, Pierre Hagberg, Henrik Hristova, Mariya Guillot, Pascale V. Neuroprotection of the hypoxic-ischemic mouse brain by human CD117(+)CD90(+)CD105(+) amniotic fluid stem cells |
title | Neuroprotection of the hypoxic-ischemic mouse brain by human CD117(+)CD90(+)CD105(+) amniotic fluid stem cells |
title_full | Neuroprotection of the hypoxic-ischemic mouse brain by human CD117(+)CD90(+)CD105(+) amniotic fluid stem cells |
title_fullStr | Neuroprotection of the hypoxic-ischemic mouse brain by human CD117(+)CD90(+)CD105(+) amniotic fluid stem cells |
title_full_unstemmed | Neuroprotection of the hypoxic-ischemic mouse brain by human CD117(+)CD90(+)CD105(+) amniotic fluid stem cells |
title_short | Neuroprotection of the hypoxic-ischemic mouse brain by human CD117(+)CD90(+)CD105(+) amniotic fluid stem cells |
title_sort | neuroprotection of the hypoxic-ischemic mouse brain by human cd117(+)cd90(+)cd105(+) amniotic fluid stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799160/ https://www.ncbi.nlm.nih.gov/pubmed/29402914 http://dx.doi.org/10.1038/s41598-018-20710-9 |
work_keys_str_mv | AT corcellimichelangelo neuroprotectionofthehypoxicischemicmousebrainbyhumancd117cd90cd105amnioticfluidstemcells AT hawkinskate neuroprotectionofthehypoxicischemicmousebrainbyhumancd117cd90cd105amnioticfluidstemcells AT vlahovafilipa neuroprotectionofthehypoxicischemicmousebrainbyhumancd117cd90cd105amnioticfluidstemcells AT hunjanavina neuroprotectionofthehypoxicischemicmousebrainbyhumancd117cd90cd105amnioticfluidstemcells AT dowdingkate neuroprotectionofthehypoxicischemicmousebrainbyhumancd117cd90cd105amnioticfluidstemcells AT decoppipaolo neuroprotectionofthehypoxicischemicmousebrainbyhumancd117cd90cd105amnioticfluidstemcells AT davidannal neuroprotectionofthehypoxicischemicmousebrainbyhumancd117cd90cd105amnioticfluidstemcells AT peeblesdonald neuroprotectionofthehypoxicischemicmousebrainbyhumancd117cd90cd105amnioticfluidstemcells AT gressenspierre neuroprotectionofthehypoxicischemicmousebrainbyhumancd117cd90cd105amnioticfluidstemcells AT hagberghenrik neuroprotectionofthehypoxicischemicmousebrainbyhumancd117cd90cd105amnioticfluidstemcells AT hristovamariya neuroprotectionofthehypoxicischemicmousebrainbyhumancd117cd90cd105amnioticfluidstemcells AT guillotpascalev neuroprotectionofthehypoxicischemicmousebrainbyhumancd117cd90cd105amnioticfluidstemcells |