Development of a yeast internal-subunit eGFP labeling strategy and its application in subunit identification in eukaryotic group II chaperonin TRiC/CCT

Unambiguous subunit assignment in a multicomponent complex is critical for thorough understanding of the machinery and its functionality. The eukaryotic group II chaperonin TRiC/CCT folds approximately 10% of cytosolic proteins and is important for the maintenance of cellular homeostasis. TRiC consi...

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Detalles Bibliográficos
Autores principales: Zang, Yunxiang, Wang, Huping, Cui, Zhicheng, Jin, Mingliang, Liu, Caixuan, Han, Wenyu, Wang, Yanxing, Cong, Yao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799240/
https://www.ncbi.nlm.nih.gov/pubmed/29403048
http://dx.doi.org/10.1038/s41598-017-18962-y

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