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The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge
A metagenomic approach was used to investigate how the microbial community composition changes when an anammox-based granular sludge reactor is seeded with nitritation-anammox biomass from a wastewater treatment plant. In the seed sample, the abundance of Candidatus Kuenenia stuttgartiensis was simi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799322/ https://www.ncbi.nlm.nih.gov/pubmed/29230681 http://dx.doi.org/10.1007/s13353-017-0418-1 |
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author | Ciesielski, Slawomir Czerwionka, Krzysztof Sobotka, Dominika Dulski, Tomasz Makinia, Jacek |
author_facet | Ciesielski, Slawomir Czerwionka, Krzysztof Sobotka, Dominika Dulski, Tomasz Makinia, Jacek |
author_sort | Ciesielski, Slawomir |
collection | PubMed |
description | A metagenomic approach was used to investigate how the microbial community composition changes when an anammox-based granular sludge reactor is seeded with nitritation-anammox biomass from a wastewater treatment plant. In the seed sample, the abundance of Candidatus Kuenenia stuttgartiensis was similar to Candidatus Jettenia caeni (12.63 vs. 11.68%). This biomass was typical in terms of microbial nitrogen conversion; both ammonia (Nitrosomonas sp.) and nitrite (Nitrospira sp.) oxidizing bacteria were detected. In the lab-scale reactor, Candidatus Kuenenia stuttgartiensis and Candidatus Jettenia caeni bacteria were also present in equal proportions (18.57 vs. 20.89%). On the contrary, Candidatus Nitrospira defluvii bacteria were highly abundant in this reactor, but no known ammonia-oxidizing bacteria were detected. In light of recent studies showing that Nitrospira sp. are capable of complete nitrification, the results presented here may well indicate that both stages of nitrification in the anammox-based granular sludge reactor were performed by this bacteria. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13353-017-0418-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5799322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-57993222018-02-12 The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge Ciesielski, Slawomir Czerwionka, Krzysztof Sobotka, Dominika Dulski, Tomasz Makinia, Jacek J Appl Genet Microbial Genetics • Original Paper A metagenomic approach was used to investigate how the microbial community composition changes when an anammox-based granular sludge reactor is seeded with nitritation-anammox biomass from a wastewater treatment plant. In the seed sample, the abundance of Candidatus Kuenenia stuttgartiensis was similar to Candidatus Jettenia caeni (12.63 vs. 11.68%). This biomass was typical in terms of microbial nitrogen conversion; both ammonia (Nitrosomonas sp.) and nitrite (Nitrospira sp.) oxidizing bacteria were detected. In the lab-scale reactor, Candidatus Kuenenia stuttgartiensis and Candidatus Jettenia caeni bacteria were also present in equal proportions (18.57 vs. 20.89%). On the contrary, Candidatus Nitrospira defluvii bacteria were highly abundant in this reactor, but no known ammonia-oxidizing bacteria were detected. In light of recent studies showing that Nitrospira sp. are capable of complete nitrification, the results presented here may well indicate that both stages of nitrification in the anammox-based granular sludge reactor were performed by this bacteria. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13353-017-0418-1) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2017-12-11 2018 /pmc/articles/PMC5799322/ /pubmed/29230681 http://dx.doi.org/10.1007/s13353-017-0418-1 Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Microbial Genetics • Original Paper Ciesielski, Slawomir Czerwionka, Krzysztof Sobotka, Dominika Dulski, Tomasz Makinia, Jacek The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge |
title | The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge |
title_full | The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge |
title_fullStr | The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge |
title_full_unstemmed | The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge |
title_short | The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge |
title_sort | metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge |
topic | Microbial Genetics • Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799322/ https://www.ncbi.nlm.nih.gov/pubmed/29230681 http://dx.doi.org/10.1007/s13353-017-0418-1 |
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