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Insights into RNAi-based antiviral immunity in Lepidoptera: acute and persistent infections in Bombyx mori and Trichoplusia ni cell lines

The control of viral infections in insects is a current issue of major concern and RNA interference (RNAi) is considered the main antiviral immune response in this group of animals. Here we demonstrate that overexpression of key RNAi factors can help to protect insect cells against viral infections....

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Autores principales: Santos, Dulce, Wynant, Niels, Van den Brande, Stijn, Verdonckt, Thomas-Wolf, Mingels, Lina, Peeters, Paulien, Kolliopoulou, Anna, Swevers, Luc, Vanden Broeck, Jozef
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799340/
https://www.ncbi.nlm.nih.gov/pubmed/29403066
http://dx.doi.org/10.1038/s41598-018-20848-6
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author Santos, Dulce
Wynant, Niels
Van den Brande, Stijn
Verdonckt, Thomas-Wolf
Mingels, Lina
Peeters, Paulien
Kolliopoulou, Anna
Swevers, Luc
Vanden Broeck, Jozef
author_facet Santos, Dulce
Wynant, Niels
Van den Brande, Stijn
Verdonckt, Thomas-Wolf
Mingels, Lina
Peeters, Paulien
Kolliopoulou, Anna
Swevers, Luc
Vanden Broeck, Jozef
author_sort Santos, Dulce
collection PubMed
description The control of viral infections in insects is a current issue of major concern and RNA interference (RNAi) is considered the main antiviral immune response in this group of animals. Here we demonstrate that overexpression of key RNAi factors can help to protect insect cells against viral infections. In particular, we show that overexpression of Dicer2 and Argonaute2 in lepidopteran cells leads to improved defense against the acute infection of the Cricket Paralysis Virus (CrPV). We also demonstrate an important role of RNAi in the control of persistent viral infections, as the one caused by the Macula-like Latent Virus (MLV). Specifically, a direct interaction between Argonaute2 and virus-specific small RNAs is shown. Yet, while knocking down Dicer2 and Argonaute2 resulted in higher transcript levels of the persistently infecting MLV in the lepidopteran cells under investigation, overexpression of these proteins could not further reduce these levels. Taken together, our data provide deep insight into the RNAi-based interactions between insects and their viruses. In addition, our results suggest the potential use of an RNAi gain-of-function approach as an alternative strategy to obtain reduced viral-induced mortality in Lepidoptera, an insect order that encompasses multiple species of relevant economic value.
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spelling pubmed-57993402018-02-14 Insights into RNAi-based antiviral immunity in Lepidoptera: acute and persistent infections in Bombyx mori and Trichoplusia ni cell lines Santos, Dulce Wynant, Niels Van den Brande, Stijn Verdonckt, Thomas-Wolf Mingels, Lina Peeters, Paulien Kolliopoulou, Anna Swevers, Luc Vanden Broeck, Jozef Sci Rep Article The control of viral infections in insects is a current issue of major concern and RNA interference (RNAi) is considered the main antiviral immune response in this group of animals. Here we demonstrate that overexpression of key RNAi factors can help to protect insect cells against viral infections. In particular, we show that overexpression of Dicer2 and Argonaute2 in lepidopteran cells leads to improved defense against the acute infection of the Cricket Paralysis Virus (CrPV). We also demonstrate an important role of RNAi in the control of persistent viral infections, as the one caused by the Macula-like Latent Virus (MLV). Specifically, a direct interaction between Argonaute2 and virus-specific small RNAs is shown. Yet, while knocking down Dicer2 and Argonaute2 resulted in higher transcript levels of the persistently infecting MLV in the lepidopteran cells under investigation, overexpression of these proteins could not further reduce these levels. Taken together, our data provide deep insight into the RNAi-based interactions between insects and their viruses. In addition, our results suggest the potential use of an RNAi gain-of-function approach as an alternative strategy to obtain reduced viral-induced mortality in Lepidoptera, an insect order that encompasses multiple species of relevant economic value. Nature Publishing Group UK 2018-02-05 /pmc/articles/PMC5799340/ /pubmed/29403066 http://dx.doi.org/10.1038/s41598-018-20848-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Santos, Dulce
Wynant, Niels
Van den Brande, Stijn
Verdonckt, Thomas-Wolf
Mingels, Lina
Peeters, Paulien
Kolliopoulou, Anna
Swevers, Luc
Vanden Broeck, Jozef
Insights into RNAi-based antiviral immunity in Lepidoptera: acute and persistent infections in Bombyx mori and Trichoplusia ni cell lines
title Insights into RNAi-based antiviral immunity in Lepidoptera: acute and persistent infections in Bombyx mori and Trichoplusia ni cell lines
title_full Insights into RNAi-based antiviral immunity in Lepidoptera: acute and persistent infections in Bombyx mori and Trichoplusia ni cell lines
title_fullStr Insights into RNAi-based antiviral immunity in Lepidoptera: acute and persistent infections in Bombyx mori and Trichoplusia ni cell lines
title_full_unstemmed Insights into RNAi-based antiviral immunity in Lepidoptera: acute and persistent infections in Bombyx mori and Trichoplusia ni cell lines
title_short Insights into RNAi-based antiviral immunity in Lepidoptera: acute and persistent infections in Bombyx mori and Trichoplusia ni cell lines
title_sort insights into rnai-based antiviral immunity in lepidoptera: acute and persistent infections in bombyx mori and trichoplusia ni cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799340/
https://www.ncbi.nlm.nih.gov/pubmed/29403066
http://dx.doi.org/10.1038/s41598-018-20848-6
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