Cargando…
Long non-coding and coding RNAs characterization in Peripheral Blood Mononuclear Cells and Spinal Cord from Amyotrophic Lateral Sclerosis patients
Alteration in RNA metabolism, concerning both coding and long non-coding RNAs (lncRNAs), may play an important role in Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we performed a whole transcriptome RNA-seq analysis to investigate the regulation of non-coding and coding RNAs in Sp...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799454/ https://www.ncbi.nlm.nih.gov/pubmed/29402919 http://dx.doi.org/10.1038/s41598-018-20679-5 |
_version_ | 1783298003725975552 |
---|---|
author | Gagliardi, Stella Zucca, Susanna Pandini, Cecilia Diamanti, Luca Bordoni, Matteo Sproviero, Daisy Arigoni, Maddalena Olivero, Martina Pansarasa, Orietta Ceroni, Mauro Calogero, Raffaele Cereda, Cristina |
author_facet | Gagliardi, Stella Zucca, Susanna Pandini, Cecilia Diamanti, Luca Bordoni, Matteo Sproviero, Daisy Arigoni, Maddalena Olivero, Martina Pansarasa, Orietta Ceroni, Mauro Calogero, Raffaele Cereda, Cristina |
author_sort | Gagliardi, Stella |
collection | PubMed |
description | Alteration in RNA metabolism, concerning both coding and long non-coding RNAs (lncRNAs), may play an important role in Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we performed a whole transcriptome RNA-seq analysis to investigate the regulation of non-coding and coding RNAs in Sporadic ALS patients (SALS), mutated ALS patients (FUS, TARDBP and SOD1) and matched controls in Peripheral Blood Mononuclear Cells (PBMC). Selected transcripts were validated in spinal cord tissues. A total of 293 differentially expressed (DE) lncRNAs was found in SALS patients, whereas a limited amount of lncRNAs was deregulated in mutated patients. A total of 87 mRNAs was differentially expressed in SALS patients; affected genes showed an association with transcription regulation, immunity and apoptosis pathways. Taken together our data highlighted the importance of extending the knowledge on transcriptomic molecular alterations and on the significance of regulatory lncRNAs classes in the understanding of ALS disease. Our data brought the light on the importance of lncRNAs and mRNAs regulation in central and peripheral systems, offering starting points for new investigations about pathogenic mechanism involved in ALS disease. |
format | Online Article Text |
id | pubmed-5799454 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-57994542018-02-14 Long non-coding and coding RNAs characterization in Peripheral Blood Mononuclear Cells and Spinal Cord from Amyotrophic Lateral Sclerosis patients Gagliardi, Stella Zucca, Susanna Pandini, Cecilia Diamanti, Luca Bordoni, Matteo Sproviero, Daisy Arigoni, Maddalena Olivero, Martina Pansarasa, Orietta Ceroni, Mauro Calogero, Raffaele Cereda, Cristina Sci Rep Article Alteration in RNA metabolism, concerning both coding and long non-coding RNAs (lncRNAs), may play an important role in Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we performed a whole transcriptome RNA-seq analysis to investigate the regulation of non-coding and coding RNAs in Sporadic ALS patients (SALS), mutated ALS patients (FUS, TARDBP and SOD1) and matched controls in Peripheral Blood Mononuclear Cells (PBMC). Selected transcripts were validated in spinal cord tissues. A total of 293 differentially expressed (DE) lncRNAs was found in SALS patients, whereas a limited amount of lncRNAs was deregulated in mutated patients. A total of 87 mRNAs was differentially expressed in SALS patients; affected genes showed an association with transcription regulation, immunity and apoptosis pathways. Taken together our data highlighted the importance of extending the knowledge on transcriptomic molecular alterations and on the significance of regulatory lncRNAs classes in the understanding of ALS disease. Our data brought the light on the importance of lncRNAs and mRNAs regulation in central and peripheral systems, offering starting points for new investigations about pathogenic mechanism involved in ALS disease. Nature Publishing Group UK 2018-02-05 /pmc/articles/PMC5799454/ /pubmed/29402919 http://dx.doi.org/10.1038/s41598-018-20679-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Gagliardi, Stella Zucca, Susanna Pandini, Cecilia Diamanti, Luca Bordoni, Matteo Sproviero, Daisy Arigoni, Maddalena Olivero, Martina Pansarasa, Orietta Ceroni, Mauro Calogero, Raffaele Cereda, Cristina Long non-coding and coding RNAs characterization in Peripheral Blood Mononuclear Cells and Spinal Cord from Amyotrophic Lateral Sclerosis patients |
title | Long non-coding and coding RNAs characterization in Peripheral Blood Mononuclear Cells and Spinal Cord from Amyotrophic Lateral Sclerosis patients |
title_full | Long non-coding and coding RNAs characterization in Peripheral Blood Mononuclear Cells and Spinal Cord from Amyotrophic Lateral Sclerosis patients |
title_fullStr | Long non-coding and coding RNAs characterization in Peripheral Blood Mononuclear Cells and Spinal Cord from Amyotrophic Lateral Sclerosis patients |
title_full_unstemmed | Long non-coding and coding RNAs characterization in Peripheral Blood Mononuclear Cells and Spinal Cord from Amyotrophic Lateral Sclerosis patients |
title_short | Long non-coding and coding RNAs characterization in Peripheral Blood Mononuclear Cells and Spinal Cord from Amyotrophic Lateral Sclerosis patients |
title_sort | long non-coding and coding rnas characterization in peripheral blood mononuclear cells and spinal cord from amyotrophic lateral sclerosis patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799454/ https://www.ncbi.nlm.nih.gov/pubmed/29402919 http://dx.doi.org/10.1038/s41598-018-20679-5 |
work_keys_str_mv | AT gagliardistella longnoncodingandcodingrnascharacterizationinperipheralbloodmononuclearcellsandspinalcordfromamyotrophiclateralsclerosispatients AT zuccasusanna longnoncodingandcodingrnascharacterizationinperipheralbloodmononuclearcellsandspinalcordfromamyotrophiclateralsclerosispatients AT pandinicecilia longnoncodingandcodingrnascharacterizationinperipheralbloodmononuclearcellsandspinalcordfromamyotrophiclateralsclerosispatients AT diamantiluca longnoncodingandcodingrnascharacterizationinperipheralbloodmononuclearcellsandspinalcordfromamyotrophiclateralsclerosispatients AT bordonimatteo longnoncodingandcodingrnascharacterizationinperipheralbloodmononuclearcellsandspinalcordfromamyotrophiclateralsclerosispatients AT sprovierodaisy longnoncodingandcodingrnascharacterizationinperipheralbloodmononuclearcellsandspinalcordfromamyotrophiclateralsclerosispatients AT arigonimaddalena longnoncodingandcodingrnascharacterizationinperipheralbloodmononuclearcellsandspinalcordfromamyotrophiclateralsclerosispatients AT oliveromartina longnoncodingandcodingrnascharacterizationinperipheralbloodmononuclearcellsandspinalcordfromamyotrophiclateralsclerosispatients AT pansarasaorietta longnoncodingandcodingrnascharacterizationinperipheralbloodmononuclearcellsandspinalcordfromamyotrophiclateralsclerosispatients AT ceronimauro longnoncodingandcodingrnascharacterizationinperipheralbloodmononuclearcellsandspinalcordfromamyotrophiclateralsclerosispatients AT calogeroraffaele longnoncodingandcodingrnascharacterizationinperipheralbloodmononuclearcellsandspinalcordfromamyotrophiclateralsclerosispatients AT ceredacristina longnoncodingandcodingrnascharacterizationinperipheralbloodmononuclearcellsandspinalcordfromamyotrophiclateralsclerosispatients |