A slow-cycling subpopulation of melanoma cells with highly invasive properties

Melanoma is a heterogeneous tumor with different subpopulations showing different proliferation rates. Slow-cycling cells were previously identified in melanoma, but not fully biologically characterized. Using the label-retention method, we identified a subpopulation of slow-cycling cells, defined a...

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Autores principales: Perego, M, Maurer, M, Wang, J X, Shaffer, S, Müller, A C, Parapatics, K, Li, L, Hristova, D, Shin, S, Keeney, F, Liu, S, Xu, X, Raj, A, Jensen, J K, Bennett, K L, Wagner, S N, Somasundaram, R, Herlyn, M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2018
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799768/
https://www.ncbi.nlm.nih.gov/pubmed/28925403
http://dx.doi.org/10.1038/onc.2017.341
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author Perego, M
Maurer, M
Wang, J X
Shaffer, S
Müller, A C
Parapatics, K
Li, L
Hristova, D
Shin, S
Keeney, F
Liu, S
Xu, X
Raj, A
Jensen, J K
Bennett, K L
Wagner, S N
Somasundaram, R
Herlyn, M
author_facet Perego, M
Maurer, M
Wang, J X
Shaffer, S
Müller, A C
Parapatics, K
Li, L
Hristova, D
Shin, S
Keeney, F
Liu, S
Xu, X
Raj, A
Jensen, J K
Bennett, K L
Wagner, S N
Somasundaram, R
Herlyn, M
author_sort Perego, M
collection PubMed
description Melanoma is a heterogeneous tumor with different subpopulations showing different proliferation rates. Slow-cycling cells were previously identified in melanoma, but not fully biologically characterized. Using the label-retention method, we identified a subpopulation of slow-cycling cells, defined as label-retaining cells (LRC), with strong invasive properties. We demonstrate through live imaging that LRC are leaving the primary tumor mass at a very early stage and disseminate to peripheral organs. Through global proteome analyses, we identified the secreted protein SerpinE2/protease nexin-1 as causative for the highly invasive potential of LRC in melanomas.
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spelling pubmed-57997682018-02-08 A slow-cycling subpopulation of melanoma cells with highly invasive properties Perego, M Maurer, M Wang, J X Shaffer, S Müller, A C Parapatics, K Li, L Hristova, D Shin, S Keeney, F Liu, S Xu, X Raj, A Jensen, J K Bennett, K L Wagner, S N Somasundaram, R Herlyn, M Oncogene Original Article Melanoma is a heterogeneous tumor with different subpopulations showing different proliferation rates. Slow-cycling cells were previously identified in melanoma, but not fully biologically characterized. Using the label-retention method, we identified a subpopulation of slow-cycling cells, defined as label-retaining cells (LRC), with strong invasive properties. We demonstrate through live imaging that LRC are leaving the primary tumor mass at a very early stage and disseminate to peripheral organs. Through global proteome analyses, we identified the secreted protein SerpinE2/protease nexin-1 as causative for the highly invasive potential of LRC in melanomas. Nature Publishing Group 2018-01-18 2017-09-18 /pmc/articles/PMC5799768/ /pubmed/28925403 http://dx.doi.org/10.1038/onc.2017.341 Text en Copyright © 2017 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Original Article
Perego, M
Maurer, M
Wang, J X
Shaffer, S
Müller, A C
Parapatics, K
Li, L
Hristova, D
Shin, S
Keeney, F
Liu, S
Xu, X
Raj, A
Jensen, J K
Bennett, K L
Wagner, S N
Somasundaram, R
Herlyn, M
A slow-cycling subpopulation of melanoma cells with highly invasive properties
title A slow-cycling subpopulation of melanoma cells with highly invasive properties
title_full A slow-cycling subpopulation of melanoma cells with highly invasive properties
title_fullStr A slow-cycling subpopulation of melanoma cells with highly invasive properties
title_full_unstemmed A slow-cycling subpopulation of melanoma cells with highly invasive properties
title_short A slow-cycling subpopulation of melanoma cells with highly invasive properties
title_sort slow-cycling subpopulation of melanoma cells with highly invasive properties
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799768/
https://www.ncbi.nlm.nih.gov/pubmed/28925403
http://dx.doi.org/10.1038/onc.2017.341
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