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Enteral nutrition combined with glutamine promotes recovery after ileal pouch-anal anastomosis in rats

AIM: To assess the effect of enteral nutrition (EN) supplemented with glutamine on recovery after ileal pouch-anal anastomosis (IPAA) in rats, to provide an experimental basis for nutritional support in patients with ulcerative colitis (UC) after IPAA. METHODS: Male Sprague-Dawley (SD) rats were ran...

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Autores principales: Xu, Yan-Yan, He, An-Qi, Liu, Gang, Li, Kai-Yu, Liu, Jian, Liu, Tong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799859/
https://www.ncbi.nlm.nih.gov/pubmed/29434447
http://dx.doi.org/10.3748/wjg.v24.i5.583
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author Xu, Yan-Yan
He, An-Qi
Liu, Gang
Li, Kai-Yu
Liu, Jian
Liu, Tong
author_facet Xu, Yan-Yan
He, An-Qi
Liu, Gang
Li, Kai-Yu
Liu, Jian
Liu, Tong
author_sort Xu, Yan-Yan
collection PubMed
description AIM: To assess the effect of enteral nutrition (EN) supplemented with glutamine on recovery after ileal pouch-anal anastomosis (IPAA) in rats, to provide an experimental basis for nutritional support in patients with ulcerative colitis (UC) after IPAA. METHODS: Male Sprague-Dawley (SD) rats were randomly divided into three groups (n = 8) after IPAA operation using a microsurgical technique. From the third postoperative day, rats in the control group, EN group, and immune nutrition (IN) group were fed standard rat chow, short peptide EN, and short peptide EN combined with glutamine ad libitum, respectively. The rats’ general condition was observed throughout the study. Serum levels of total protein (TP), albumin (ALB), prealbumin (PA), and transferrin (TF) were detected on the 30th postoperative day, using an automatic biochemical analyzer. The ileal pouch mucosa was stained with hematoxylin and eosin (HE), and occludin protein levels were detected by immunohistochemistry. RESULTS: The body weight of rats in the EN group (359.20 ± 10.06 g) was significantly higher than that in the control group (344.00 ± 9.66 g) (P < 0.05) and lower than that in the IN group (373.60 ± 9.86 g) (P < 0.05) on the 30th postoperative day. The levels of serum TP, ALB, PA, and TF in the EN group were significantly higher than those in the control group (P < 0.01 for all) and lower than those in the IN group (P < 0.05 for all). Histopathological score (EN: 0.80 ± 0.37; IN: 0.60 ± 0.40; control group: 2.29 ± 0.18) and expression level of occludin protein (EN: 0.182 ± 0.054; IN: 0.188 ± 0.048; control group: 0.127 ± 0.032) were significantly lower in the control group compared with the EN and IN groups (P < 0.05 for all), but there were no significant differences between the latter two groups (P > 0.05 for all). CONCLUSION: EN combined with glutamine may effectively improve nutritional status after IPAA. Our results suggest a benefit of glutamine supplementation in EN for UC patients undergoing IPAA, although human studies are required to confirm this finding.
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spelling pubmed-57998592018-02-13 Enteral nutrition combined with glutamine promotes recovery after ileal pouch-anal anastomosis in rats Xu, Yan-Yan He, An-Qi Liu, Gang Li, Kai-Yu Liu, Jian Liu, Tong World J Gastroenterol Basic Study AIM: To assess the effect of enteral nutrition (EN) supplemented with glutamine on recovery after ileal pouch-anal anastomosis (IPAA) in rats, to provide an experimental basis for nutritional support in patients with ulcerative colitis (UC) after IPAA. METHODS: Male Sprague-Dawley (SD) rats were randomly divided into three groups (n = 8) after IPAA operation using a microsurgical technique. From the third postoperative day, rats in the control group, EN group, and immune nutrition (IN) group were fed standard rat chow, short peptide EN, and short peptide EN combined with glutamine ad libitum, respectively. The rats’ general condition was observed throughout the study. Serum levels of total protein (TP), albumin (ALB), prealbumin (PA), and transferrin (TF) were detected on the 30th postoperative day, using an automatic biochemical analyzer. The ileal pouch mucosa was stained with hematoxylin and eosin (HE), and occludin protein levels were detected by immunohistochemistry. RESULTS: The body weight of rats in the EN group (359.20 ± 10.06 g) was significantly higher than that in the control group (344.00 ± 9.66 g) (P < 0.05) and lower than that in the IN group (373.60 ± 9.86 g) (P < 0.05) on the 30th postoperative day. The levels of serum TP, ALB, PA, and TF in the EN group were significantly higher than those in the control group (P < 0.01 for all) and lower than those in the IN group (P < 0.05 for all). Histopathological score (EN: 0.80 ± 0.37; IN: 0.60 ± 0.40; control group: 2.29 ± 0.18) and expression level of occludin protein (EN: 0.182 ± 0.054; IN: 0.188 ± 0.048; control group: 0.127 ± 0.032) were significantly lower in the control group compared with the EN and IN groups (P < 0.05 for all), but there were no significant differences between the latter two groups (P > 0.05 for all). CONCLUSION: EN combined with glutamine may effectively improve nutritional status after IPAA. Our results suggest a benefit of glutamine supplementation in EN for UC patients undergoing IPAA, although human studies are required to confirm this finding. Baishideng Publishing Group Inc 2018-02-07 2018-02-07 /pmc/articles/PMC5799859/ /pubmed/29434447 http://dx.doi.org/10.3748/wjg.v24.i5.583 Text en ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Basic Study
Xu, Yan-Yan
He, An-Qi
Liu, Gang
Li, Kai-Yu
Liu, Jian
Liu, Tong
Enteral nutrition combined with glutamine promotes recovery after ileal pouch-anal anastomosis in rats
title Enteral nutrition combined with glutamine promotes recovery after ileal pouch-anal anastomosis in rats
title_full Enteral nutrition combined with glutamine promotes recovery after ileal pouch-anal anastomosis in rats
title_fullStr Enteral nutrition combined with glutamine promotes recovery after ileal pouch-anal anastomosis in rats
title_full_unstemmed Enteral nutrition combined with glutamine promotes recovery after ileal pouch-anal anastomosis in rats
title_short Enteral nutrition combined with glutamine promotes recovery after ileal pouch-anal anastomosis in rats
title_sort enteral nutrition combined with glutamine promotes recovery after ileal pouch-anal anastomosis in rats
topic Basic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799859/
https://www.ncbi.nlm.nih.gov/pubmed/29434447
http://dx.doi.org/10.3748/wjg.v24.i5.583
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