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Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta

BACKGROUND: Studies in which mesenchymal stromal cells (MSC) from the placenta are compared with multiple MSC types from other sources are rare. The chorionic plate of the human placenta is mainly composed of fetal blood vessels embedded in fetal stroma tissue, lined by trophoblastic cells and organ...

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Autores principales: Ventura Ferreira, Mónica S., Bienert, Michaela, Müller, Katrin, Rath, Björn, Goecke, Tamme, Opländer, Christian, Braunschweig, Till, Mela, Petra, Brümmendorf, Tim H., Beier, Fabian, Neuss, Sabine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800083/
https://www.ncbi.nlm.nih.gov/pubmed/29402304
http://dx.doi.org/10.1186/s13287-017-0757-1
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author Ventura Ferreira, Mónica S.
Bienert, Michaela
Müller, Katrin
Rath, Björn
Goecke, Tamme
Opländer, Christian
Braunschweig, Till
Mela, Petra
Brümmendorf, Tim H.
Beier, Fabian
Neuss, Sabine
author_facet Ventura Ferreira, Mónica S.
Bienert, Michaela
Müller, Katrin
Rath, Björn
Goecke, Tamme
Opländer, Christian
Braunschweig, Till
Mela, Petra
Brümmendorf, Tim H.
Beier, Fabian
Neuss, Sabine
author_sort Ventura Ferreira, Mónica S.
collection PubMed
description BACKGROUND: Studies in which mesenchymal stromal cells (MSC) from the placenta are compared with multiple MSC types from other sources are rare. The chorionic plate of the human placenta is mainly composed of fetal blood vessels embedded in fetal stroma tissue, lined by trophoblastic cells and organized into chorionic villi (CV) structures. METHODS: We comprehensively characterized human MSC collected from postnatal human chorionic villi of placenta (CV-MSC) by analyzing their growth and proliferation potential, differentiation, immunophenotype, extracellular matrix production, telomere length, aging phenotype, and plasticity. RESULTS: Immunophenotypic characterization of CV-MSC confirmed the typical MSC marker expression as defined by the International Society for Cellular Therapy. The surface marker profile was consistent with increased potential for proliferation, vascular localization, and early myogenic marker expression. CV-MSC retained multilineage differentiation potential and extracellular matrix remodeling properties. They have undergone reduced telomere loss and delayed onset of cellular senescence as they aged in vitro compared to three other MSC sources. We present evidence that increased human telomerase reverse transcriptase gene expression could not explain the exceptional telomere maintenance and senescence onset delay in cultured CV-MSC. Our in-vitro tumorigenesis detection assay suggests that CV-MSC are not prone to undergo malignant transformation during long-term in-vitro culture. Besides SOX2 expression, no other pluripotency features were observed in early and late passages of CV-MSC. CONCLUSIONS: Our work brings forward two remarkable characteristics of CV-MSC, the first being their extended life span as a result of delayed replicative senescence and the second being a delayed aged phenotype characterized by improved telomere length maintenance. MSC from human placenta are very attractive candidates for stem cell-based therapy applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-017-0757-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-58000832018-02-13 Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta Ventura Ferreira, Mónica S. Bienert, Michaela Müller, Katrin Rath, Björn Goecke, Tamme Opländer, Christian Braunschweig, Till Mela, Petra Brümmendorf, Tim H. Beier, Fabian Neuss, Sabine Stem Cell Res Ther Research BACKGROUND: Studies in which mesenchymal stromal cells (MSC) from the placenta are compared with multiple MSC types from other sources are rare. The chorionic plate of the human placenta is mainly composed of fetal blood vessels embedded in fetal stroma tissue, lined by trophoblastic cells and organized into chorionic villi (CV) structures. METHODS: We comprehensively characterized human MSC collected from postnatal human chorionic villi of placenta (CV-MSC) by analyzing their growth and proliferation potential, differentiation, immunophenotype, extracellular matrix production, telomere length, aging phenotype, and plasticity. RESULTS: Immunophenotypic characterization of CV-MSC confirmed the typical MSC marker expression as defined by the International Society for Cellular Therapy. The surface marker profile was consistent with increased potential for proliferation, vascular localization, and early myogenic marker expression. CV-MSC retained multilineage differentiation potential and extracellular matrix remodeling properties. They have undergone reduced telomere loss and delayed onset of cellular senescence as they aged in vitro compared to three other MSC sources. We present evidence that increased human telomerase reverse transcriptase gene expression could not explain the exceptional telomere maintenance and senescence onset delay in cultured CV-MSC. Our in-vitro tumorigenesis detection assay suggests that CV-MSC are not prone to undergo malignant transformation during long-term in-vitro culture. Besides SOX2 expression, no other pluripotency features were observed in early and late passages of CV-MSC. CONCLUSIONS: Our work brings forward two remarkable characteristics of CV-MSC, the first being their extended life span as a result of delayed replicative senescence and the second being a delayed aged phenotype characterized by improved telomere length maintenance. MSC from human placenta are very attractive candidates for stem cell-based therapy applications. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-017-0757-1) contains supplementary material, which is available to authorized users. BioMed Central 2018-02-05 /pmc/articles/PMC5800083/ /pubmed/29402304 http://dx.doi.org/10.1186/s13287-017-0757-1 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ventura Ferreira, Mónica S.
Bienert, Michaela
Müller, Katrin
Rath, Björn
Goecke, Tamme
Opländer, Christian
Braunschweig, Till
Mela, Petra
Brümmendorf, Tim H.
Beier, Fabian
Neuss, Sabine
Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta
title Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta
title_full Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta
title_fullStr Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta
title_full_unstemmed Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta
title_short Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta
title_sort comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800083/
https://www.ncbi.nlm.nih.gov/pubmed/29402304
http://dx.doi.org/10.1186/s13287-017-0757-1
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