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1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells

More studies that are focused on the bioeffects of radio-frequency (RF) electromagnetic radiation that is generated from the communication devices, but there were few reports with confirmed results about the bioeffects of RF radiation on reproductive cells. To explore the effects of 1950 MHz RF elec...

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Autores principales: Lin, Yan-Yun, Wu, Tao, Liu, Jun-Ye, Gao, Peng, Li, Kang-Chu, Guo, Qi-Yan, Yuan, Meng, Lang, Hai-Yang, Zeng, Li-Hua, Guo, Guo-Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800117/
https://www.ncbi.nlm.nih.gov/pubmed/29295490
http://dx.doi.org/10.3390/ijerph15010017
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author Lin, Yan-Yun
Wu, Tao
Liu, Jun-Ye
Gao, Peng
Li, Kang-Chu
Guo, Qi-Yan
Yuan, Meng
Lang, Hai-Yang
Zeng, Li-Hua
Guo, Guo-Zhen
author_facet Lin, Yan-Yun
Wu, Tao
Liu, Jun-Ye
Gao, Peng
Li, Kang-Chu
Guo, Qi-Yan
Yuan, Meng
Lang, Hai-Yang
Zeng, Li-Hua
Guo, Guo-Zhen
author_sort Lin, Yan-Yun
collection PubMed
description More studies that are focused on the bioeffects of radio-frequency (RF) electromagnetic radiation that is generated from the communication devices, but there were few reports with confirmed results about the bioeffects of RF radiation on reproductive cells. To explore the effects of 1950 MHz RF electromagnetic radiation (EMR) on mouse Leydig (TM3) cells. TM3 cells were irradiated or sham-irradiated continuously for 24 h by the specific absorption rate (SAR) 3 W/kg radiation. At 0, 1, 2, 3, 4, and 5 days after irradiation, cell proliferation was detected by cell counting kit-8 (CCK-8) method, cell cycle distribution, percentage of apoptosis, and cellular reactive oxygen species (ROS) were examined by flow cytometry, Testosterone level was measured using enzyme-linked immunosorbent assay (ELISA) assay, messenger ribonucleic acid (mRNA) expression level of steroidogenic acute regulatory protein (StAR) and P450scc in TM3 cells was detected by real-time polymerase chain reaction (PCR). After being irradiated for 24 h, cell proliferation obviously decreased and cell cycle distribution, secretion capacity of Testosterone, and P450scc mRNA level were reduced. While cell apoptosis, ROS, and StAR mRNA level did not change significantly. The current results indicated that 24 h of exposure at 1950 MHz 3 W/kg radiation could cause some adverse effects on TM3 cells proliferation and Testosterone secretion, further studies about the biological effects in the reproductive system that are induced by RF radiation are also needed.
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spelling pubmed-58001172018-02-06 1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells Lin, Yan-Yun Wu, Tao Liu, Jun-Ye Gao, Peng Li, Kang-Chu Guo, Qi-Yan Yuan, Meng Lang, Hai-Yang Zeng, Li-Hua Guo, Guo-Zhen Int J Environ Res Public Health Article More studies that are focused on the bioeffects of radio-frequency (RF) electromagnetic radiation that is generated from the communication devices, but there were few reports with confirmed results about the bioeffects of RF radiation on reproductive cells. To explore the effects of 1950 MHz RF electromagnetic radiation (EMR) on mouse Leydig (TM3) cells. TM3 cells were irradiated or sham-irradiated continuously for 24 h by the specific absorption rate (SAR) 3 W/kg radiation. At 0, 1, 2, 3, 4, and 5 days after irradiation, cell proliferation was detected by cell counting kit-8 (CCK-8) method, cell cycle distribution, percentage of apoptosis, and cellular reactive oxygen species (ROS) were examined by flow cytometry, Testosterone level was measured using enzyme-linked immunosorbent assay (ELISA) assay, messenger ribonucleic acid (mRNA) expression level of steroidogenic acute regulatory protein (StAR) and P450scc in TM3 cells was detected by real-time polymerase chain reaction (PCR). After being irradiated for 24 h, cell proliferation obviously decreased and cell cycle distribution, secretion capacity of Testosterone, and P450scc mRNA level were reduced. While cell apoptosis, ROS, and StAR mRNA level did not change significantly. The current results indicated that 24 h of exposure at 1950 MHz 3 W/kg radiation could cause some adverse effects on TM3 cells proliferation and Testosterone secretion, further studies about the biological effects in the reproductive system that are induced by RF radiation are also needed. MDPI 2017-12-23 2018-01 /pmc/articles/PMC5800117/ /pubmed/29295490 http://dx.doi.org/10.3390/ijerph15010017 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lin, Yan-Yun
Wu, Tao
Liu, Jun-Ye
Gao, Peng
Li, Kang-Chu
Guo, Qi-Yan
Yuan, Meng
Lang, Hai-Yang
Zeng, Li-Hua
Guo, Guo-Zhen
1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells
title 1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells
title_full 1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells
title_fullStr 1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells
title_full_unstemmed 1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells
title_short 1950MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells
title_sort 1950mhz radio frequency electromagnetic radiation inhibits testosterone secretion of mouse leydig cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800117/
https://www.ncbi.nlm.nih.gov/pubmed/29295490
http://dx.doi.org/10.3390/ijerph15010017
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