Molecular detection of airborne Emergomyces africanus, a thermally dimorphic fungal pathogen, in Cape Town, South Africa

Emergomyces africanus is a thermally dimorphic fungus that causes a systemic mycosis in immunocompromised persons in South Africa. Infection is presumed to follow inhalation of airborne propagules. We developed a quantitative PCR protocol able to detect as few as 5 Es. africanus propagules per day....

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Detalles Bibliográficos
Autores principales: Schwartz, Ilan S., McLoud, Josh D., Berman, Dilys, Botha, Alfred, Lerm, Barbra, Colebunders, Robert, Levetin, Estelle, Kenyon, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800596/
https://www.ncbi.nlm.nih.gov/pubmed/29357352
http://dx.doi.org/10.1371/journal.pntd.0006174
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author Schwartz, Ilan S.
McLoud, Josh D.
Berman, Dilys
Botha, Alfred
Lerm, Barbra
Colebunders, Robert
Levetin, Estelle
Kenyon, Chris
author_facet Schwartz, Ilan S.
McLoud, Josh D.
Berman, Dilys
Botha, Alfred
Lerm, Barbra
Colebunders, Robert
Levetin, Estelle
Kenyon, Chris
author_sort Schwartz, Ilan S.
collection PubMed
description Emergomyces africanus is a thermally dimorphic fungus that causes a systemic mycosis in immunocompromised persons in South Africa. Infection is presumed to follow inhalation of airborne propagules. We developed a quantitative PCR protocol able to detect as few as 5 Es. africanus propagules per day. Samples were collected in Cape Town, South Africa over 50 weeks by a Burkard spore trap with an alternate orifice. We detected Es. africanus in air samples from 34 days (10%) distributed over 11 weeks. These results suggest environmental exposure to airborne Es. africanus propagules occurs more commonly in endemic areas than previously appreciated.
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spelling pubmed-58005962018-02-23 Molecular detection of airborne Emergomyces africanus, a thermally dimorphic fungal pathogen, in Cape Town, South Africa Schwartz, Ilan S. McLoud, Josh D. Berman, Dilys Botha, Alfred Lerm, Barbra Colebunders, Robert Levetin, Estelle Kenyon, Chris PLoS Negl Trop Dis Research Article Emergomyces africanus is a thermally dimorphic fungus that causes a systemic mycosis in immunocompromised persons in South Africa. Infection is presumed to follow inhalation of airborne propagules. We developed a quantitative PCR protocol able to detect as few as 5 Es. africanus propagules per day. Samples were collected in Cape Town, South Africa over 50 weeks by a Burkard spore trap with an alternate orifice. We detected Es. africanus in air samples from 34 days (10%) distributed over 11 weeks. These results suggest environmental exposure to airborne Es. africanus propagules occurs more commonly in endemic areas than previously appreciated. Public Library of Science 2018-01-22 /pmc/articles/PMC5800596/ /pubmed/29357352 http://dx.doi.org/10.1371/journal.pntd.0006174 Text en © 2018 Schwartz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Schwartz, Ilan S.
McLoud, Josh D.
Berman, Dilys
Botha, Alfred
Lerm, Barbra
Colebunders, Robert
Levetin, Estelle
Kenyon, Chris
Molecular detection of airborne Emergomyces africanus, a thermally dimorphic fungal pathogen, in Cape Town, South Africa
title Molecular detection of airborne Emergomyces africanus, a thermally dimorphic fungal pathogen, in Cape Town, South Africa
title_full Molecular detection of airborne Emergomyces africanus, a thermally dimorphic fungal pathogen, in Cape Town, South Africa
title_fullStr Molecular detection of airborne Emergomyces africanus, a thermally dimorphic fungal pathogen, in Cape Town, South Africa
title_full_unstemmed Molecular detection of airborne Emergomyces africanus, a thermally dimorphic fungal pathogen, in Cape Town, South Africa
title_short Molecular detection of airborne Emergomyces africanus, a thermally dimorphic fungal pathogen, in Cape Town, South Africa
title_sort molecular detection of airborne emergomyces africanus, a thermally dimorphic fungal pathogen, in cape town, south africa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800596/
https://www.ncbi.nlm.nih.gov/pubmed/29357352
http://dx.doi.org/10.1371/journal.pntd.0006174
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