MALAT1/miR-101-3p/MCL1 axis mediates cisplatin resistance in lung cancer

In this study, we investigated the mechanism by which lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) mediates cisplatin resistance in lung cancer. Lung cancer patients with high MALAT1 levels were associated with cisplatin resistance and low overall survival. Moreover, cispla...

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Autores principales: Wang, Huaqi, Wang, Li, Zhang, Guojun, Lu, Chunya, Chu, Heying, Yang, Rui, Zhao, Guoqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800919/
https://www.ncbi.nlm.nih.gov/pubmed/29484127
http://dx.doi.org/10.18632/oncotarget.23483
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author Wang, Huaqi
Wang, Li
Zhang, Guojun
Lu, Chunya
Chu, Heying
Yang, Rui
Zhao, Guoqiang
author_facet Wang, Huaqi
Wang, Li
Zhang, Guojun
Lu, Chunya
Chu, Heying
Yang, Rui
Zhao, Guoqiang
author_sort Wang, Huaqi
collection PubMed
description In this study, we investigated the mechanism by which lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) mediates cisplatin resistance in lung cancer. Lung cancer patients with high MALAT1 levels were associated with cisplatin resistance and low overall survival. Moreover, cisplatin-resistant A549/DDP cells showed higher MALAT1 expression than cisplatin-sensitive lung cancer cells (A549, H460, H1299 and SPC-A1). Dual luciferase reporter and RNA immunoprecipitation assays showed direct binding of miR-101-3p to MALAT1. MALAT1 knockdown in lung cancer cells resulted in miR-101-3p upregulation and increased cisplatin sensitivity. In addition, miR-101-3p decreased myeloid cell leukemia 1 (MCL1) expression by binding to the 3’-untranslated region (3’-UTR) of its mRNA. These results demonstrate that MALAT1/miR-101-3p/MCL1 signaling underlies cisplatin resistance in lung cancer.
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spelling pubmed-58009192018-02-26 MALAT1/miR-101-3p/MCL1 axis mediates cisplatin resistance in lung cancer Wang, Huaqi Wang, Li Zhang, Guojun Lu, Chunya Chu, Heying Yang, Rui Zhao, Guoqiang Oncotarget Research Paper In this study, we investigated the mechanism by which lncRNA metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) mediates cisplatin resistance in lung cancer. Lung cancer patients with high MALAT1 levels were associated with cisplatin resistance and low overall survival. Moreover, cisplatin-resistant A549/DDP cells showed higher MALAT1 expression than cisplatin-sensitive lung cancer cells (A549, H460, H1299 and SPC-A1). Dual luciferase reporter and RNA immunoprecipitation assays showed direct binding of miR-101-3p to MALAT1. MALAT1 knockdown in lung cancer cells resulted in miR-101-3p upregulation and increased cisplatin sensitivity. In addition, miR-101-3p decreased myeloid cell leukemia 1 (MCL1) expression by binding to the 3’-untranslated region (3’-UTR) of its mRNA. These results demonstrate that MALAT1/miR-101-3p/MCL1 signaling underlies cisplatin resistance in lung cancer. Impact Journals LLC 2017-12-14 /pmc/articles/PMC5800919/ /pubmed/29484127 http://dx.doi.org/10.18632/oncotarget.23483 Text en Copyright: © 2018 Wang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Wang, Huaqi
Wang, Li
Zhang, Guojun
Lu, Chunya
Chu, Heying
Yang, Rui
Zhao, Guoqiang
MALAT1/miR-101-3p/MCL1 axis mediates cisplatin resistance in lung cancer
title MALAT1/miR-101-3p/MCL1 axis mediates cisplatin resistance in lung cancer
title_full MALAT1/miR-101-3p/MCL1 axis mediates cisplatin resistance in lung cancer
title_fullStr MALAT1/miR-101-3p/MCL1 axis mediates cisplatin resistance in lung cancer
title_full_unstemmed MALAT1/miR-101-3p/MCL1 axis mediates cisplatin resistance in lung cancer
title_short MALAT1/miR-101-3p/MCL1 axis mediates cisplatin resistance in lung cancer
title_sort malat1/mir-101-3p/mcl1 axis mediates cisplatin resistance in lung cancer
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800919/
https://www.ncbi.nlm.nih.gov/pubmed/29484127
http://dx.doi.org/10.18632/oncotarget.23483
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