Orthologous CRISPR-Cas9 enzymes for Combinatorial Genetic Screens

Combinatorial genetic screening using CRISPR-Cas9 is a useful approach to uncover redundant genes and to explore complex gene networks. However, current approaches suffer from interference between the single-guide RNAs (sgRNAs) and from limited gene targeting activity. To increase the efficiency of...

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Detalles Bibliográficos
Autores principales: Najm, Fadi J, Strand, Christine, Donovan, Katherine F, Hegde, Mudra, Sanson, Kendall R, Vaimberg, Emma W, Sullender, Meagan E, Hartenian, Ella, Kalani, Zohra, Fusi, Nicolo, Listgarten, Jennifer, Younger, Scott T, Bernstein, Bradley E, Root, David E, Doench, John G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800952/
https://www.ncbi.nlm.nih.gov/pubmed/29251726
http://dx.doi.org/10.1038/nbt.4048

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5800952/
https://www.ncbi.nlm.nih.gov/pubmed/29251726
http://dx.doi.org/10.1038/nbt.4048

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