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Functional Interrogation and Mining of Natively-Paired Human V(H):V(L) Antibody Repertoires
We present a technology to screen natively-paired human antibody repertoires from millions of B cells. Libraries of natively-paired variable region heavy and light (V(H):V(L)) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801115/ https://www.ncbi.nlm.nih.gov/pubmed/29309060 http://dx.doi.org/10.1038/nbt.4052 |
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| author | Wang, Bo DeKosky, Brandon J. Timm, Morgan R. Lee, Jiwon Normandin, Erica Misasi, John Kong, Rui McDaniel, Jonathan R. Delidakis, George Leigh, Kendra E. Niezold, Thomas Choi, Chang W. Viox, Elise G. Fahad, Ahmed Cagigi, Alberto Ploquin, Aurélie Leung, Kwanyee Yang, Eun S. Kong, Wing-Pui Voss, William N. Schmidt, Aaron G. Moody, M. Anthony Ambrozak, David R. Henry, Amy R. Laboune, Farida Ledgerwood, Julie E. Graham, Barney S. Connors, Mark Douek, Daniel C. Sullivan, Nancy J. Ellington, Andrew D. Mascola, John R. Georgiou, George |
| author_facet | Wang, Bo DeKosky, Brandon J. Timm, Morgan R. Lee, Jiwon Normandin, Erica Misasi, John Kong, Rui McDaniel, Jonathan R. Delidakis, George Leigh, Kendra E. Niezold, Thomas Choi, Chang W. Viox, Elise G. Fahad, Ahmed Cagigi, Alberto Ploquin, Aurélie Leung, Kwanyee Yang, Eun S. Kong, Wing-Pui Voss, William N. Schmidt, Aaron G. Moody, M. Anthony Ambrozak, David R. Henry, Amy R. Laboune, Farida Ledgerwood, Julie E. Graham, Barney S. Connors, Mark Douek, Daniel C. Sullivan, Nancy J. Ellington, Andrew D. Mascola, John R. Georgiou, George |
| author_sort | Wang, Bo |
| collection | PubMed |
| description | We present a technology to screen natively-paired human antibody repertoires from millions of B cells. Libraries of natively-paired variable region heavy and light (V(H):V(L)) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin. |
| format | Online Article Text |
| id | pubmed-5801115 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2018 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-58011152018-07-08 Functional Interrogation and Mining of Natively-Paired Human V(H):V(L) Antibody Repertoires Wang, Bo DeKosky, Brandon J. Timm, Morgan R. Lee, Jiwon Normandin, Erica Misasi, John Kong, Rui McDaniel, Jonathan R. Delidakis, George Leigh, Kendra E. Niezold, Thomas Choi, Chang W. Viox, Elise G. Fahad, Ahmed Cagigi, Alberto Ploquin, Aurélie Leung, Kwanyee Yang, Eun S. Kong, Wing-Pui Voss, William N. Schmidt, Aaron G. Moody, M. Anthony Ambrozak, David R. Henry, Amy R. Laboune, Farida Ledgerwood, Julie E. Graham, Barney S. Connors, Mark Douek, Daniel C. Sullivan, Nancy J. Ellington, Andrew D. Mascola, John R. Georgiou, George Nat Biotechnol Article We present a technology to screen natively-paired human antibody repertoires from millions of B cells. Libraries of natively-paired variable region heavy and light (V(H):V(L)) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin. 2018-01-08 2018-02 /pmc/articles/PMC5801115/ /pubmed/29309060 http://dx.doi.org/10.1038/nbt.4052 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Wang, Bo DeKosky, Brandon J. Timm, Morgan R. Lee, Jiwon Normandin, Erica Misasi, John Kong, Rui McDaniel, Jonathan R. Delidakis, George Leigh, Kendra E. Niezold, Thomas Choi, Chang W. Viox, Elise G. Fahad, Ahmed Cagigi, Alberto Ploquin, Aurélie Leung, Kwanyee Yang, Eun S. Kong, Wing-Pui Voss, William N. Schmidt, Aaron G. Moody, M. Anthony Ambrozak, David R. Henry, Amy R. Laboune, Farida Ledgerwood, Julie E. Graham, Barney S. Connors, Mark Douek, Daniel C. Sullivan, Nancy J. Ellington, Andrew D. Mascola, John R. Georgiou, George Functional Interrogation and Mining of Natively-Paired Human V(H):V(L) Antibody Repertoires |
| title | Functional Interrogation and Mining of Natively-Paired Human V(H):V(L) Antibody Repertoires |
| title_full | Functional Interrogation and Mining of Natively-Paired Human V(H):V(L) Antibody Repertoires |
| title_fullStr | Functional Interrogation and Mining of Natively-Paired Human V(H):V(L) Antibody Repertoires |
| title_full_unstemmed | Functional Interrogation and Mining of Natively-Paired Human V(H):V(L) Antibody Repertoires |
| title_short | Functional Interrogation and Mining of Natively-Paired Human V(H):V(L) Antibody Repertoires |
| title_sort | functional interrogation and mining of natively-paired human v(h):v(l) antibody repertoires |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801115/ https://www.ncbi.nlm.nih.gov/pubmed/29309060 http://dx.doi.org/10.1038/nbt.4052 |
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