Functional Interrogation and Mining of Natively-Paired Human V(H):V(L) Antibody Repertoires

We present a technology to screen natively-paired human antibody repertoires from millions of B cells. Libraries of natively-paired variable region heavy and light (V(H):V(L)) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we...

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Detalles Bibliográficos
Autores principales: Wang, Bo, DeKosky, Brandon J., Timm, Morgan R., Lee, Jiwon, Normandin, Erica, Misasi, John, Kong, Rui, McDaniel, Jonathan R., Delidakis, George, Leigh, Kendra E., Niezold, Thomas, Choi, Chang W., Viox, Elise G., Fahad, Ahmed, Cagigi, Alberto, Ploquin, Aurélie, Leung, Kwanyee, Yang, Eun S., Kong, Wing-Pui, Voss, William N., Schmidt, Aaron G., Moody, M. Anthony, Ambrozak, David R., Henry, Amy R., Laboune, Farida, Ledgerwood, Julie E., Graham, Barney S., Connors, Mark, Douek, Daniel C., Sullivan, Nancy J., Ellington, Andrew D., Mascola, John R., Georgiou, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801115/
https://www.ncbi.nlm.nih.gov/pubmed/29309060
http://dx.doi.org/10.1038/nbt.4052

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