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Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond

More than 40 years ago, Howard Green's laboratory developed a method for long‐term expansion of primary human epidermal keratinocytes by co‐culture with 3T3 mouse embryonic fibroblasts. This was a breakthrough for in vitro cultivation of cells from human skin and later for other epithelia: it l...

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Autores principales: Hynds, Robert E, Bonfanti, Paola, Janes, Sam M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801505/
https://www.ncbi.nlm.nih.gov/pubmed/29288165
http://dx.doi.org/10.15252/emmm.201708213
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author Hynds, Robert E
Bonfanti, Paola
Janes, Sam M
author_facet Hynds, Robert E
Bonfanti, Paola
Janes, Sam M
author_sort Hynds, Robert E
collection PubMed
description More than 40 years ago, Howard Green's laboratory developed a method for long‐term expansion of primary human epidermal keratinocytes by co‐culture with 3T3 mouse embryonic fibroblasts. This was a breakthrough for in vitro cultivation of cells from human skin and later for other epithelia: it led to the first stem cell therapy using cultured cells and has vastly increased our understanding of epithelial stem cell biology. In recent years, new methods to expand epithelial cells as three‐dimensional organoids have provided novel means to investigate the functions of these cells in health and disease. Here, we outline the history of stratified epithelial stem cell culture and the application of cultured epithelial cells in clinical therapies. We further discuss the derivation of organoids from other types of epithelia and the challenges that remain for the translation of novel stem cell therapies toward clinical use.
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spelling pubmed-58015052018-02-15 Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond Hynds, Robert E Bonfanti, Paola Janes, Sam M EMBO Mol Med Review More than 40 years ago, Howard Green's laboratory developed a method for long‐term expansion of primary human epidermal keratinocytes by co‐culture with 3T3 mouse embryonic fibroblasts. This was a breakthrough for in vitro cultivation of cells from human skin and later for other epithelia: it led to the first stem cell therapy using cultured cells and has vastly increased our understanding of epithelial stem cell biology. In recent years, new methods to expand epithelial cells as three‐dimensional organoids have provided novel means to investigate the functions of these cells in health and disease. Here, we outline the history of stratified epithelial stem cell culture and the application of cultured epithelial cells in clinical therapies. We further discuss the derivation of organoids from other types of epithelia and the challenges that remain for the translation of novel stem cell therapies toward clinical use. John Wiley and Sons Inc. 2017-12-29 2018-02 /pmc/articles/PMC5801505/ /pubmed/29288165 http://dx.doi.org/10.15252/emmm.201708213 Text en © 2017 The Authors. Published under the terms of the CC BY 4.0 license This is an open access article under the terms of the Creative Commons Attribution 4.0 (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Hynds, Robert E
Bonfanti, Paola
Janes, Sam M
Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond
title Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond
title_full Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond
title_fullStr Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond
title_full_unstemmed Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond
title_short Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond
title_sort regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801505/
https://www.ncbi.nlm.nih.gov/pubmed/29288165
http://dx.doi.org/10.15252/emmm.201708213
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