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Effect of hydroxychloroquine on oxidative/nitrosative status and angiogenesis in endothelial cells under high glucose condition

[Image: see text] Introduction: Under the diabetic condition, sustained production of oxidative/nitrosative stress results in irreversible vascular injuries. A great number of diabetic pathologies, such as inefficient or aberrant neo-angiogenesis emerge following chronic hyperglycemic condition. Lac...

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Autores principales: Rezabakhsh, Aysa, Montazersaheb, Soheila, Nabat, Elahe, Hassanpour, Mehdi, Montaseri, Azadeh, Malekinejad, Hassan, Movassaghpour, Ali Akbar, Rahbarghazi, Reza, Garjani, Alireza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801533/
https://www.ncbi.nlm.nih.gov/pubmed/29435429
http://dx.doi.org/10.15171/bi.2017.26
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author Rezabakhsh, Aysa
Montazersaheb, Soheila
Nabat, Elahe
Hassanpour, Mehdi
Montaseri, Azadeh
Malekinejad, Hassan
Movassaghpour, Ali Akbar
Rahbarghazi, Reza
Garjani, Alireza
author_facet Rezabakhsh, Aysa
Montazersaheb, Soheila
Nabat, Elahe
Hassanpour, Mehdi
Montaseri, Azadeh
Malekinejad, Hassan
Movassaghpour, Ali Akbar
Rahbarghazi, Reza
Garjani, Alireza
author_sort Rezabakhsh, Aysa
collection PubMed
description [Image: see text] Introduction: Under the diabetic condition, sustained production of oxidative/nitrosative stress results in irreversible vascular injuries. A great number of diabetic pathologies, such as inefficient or aberrant neo-angiogenesis emerge following chronic hyperglycemic condition. Lack of enough data exists regarding hydroxychloroquine (HCQ) contribution on angiogenesis during diabetes mellitus. Methods: To better address whether HCQ could blunt or exacerbate oxidative status and angiogenesis under high glucose condition (HCG), human umbilical vein endothelial cells (HUVECs) were exposed to 30 µM HCQ in combination with 30 mM glucose over a course of 72 hours. Viability was measured was evaluated by MTT assay. We used Griess method and TBARS assay to monitor changes in the levels of NO and MDA followed by flow cytometric analysis of ROS using DCFDA. To show the impact of HCQ on cell motility and in vitro angiogenic properties, we exploited routine scratch test and in vitro tubulogenesis, respectively. Results: Our data showed that HCQ diminished cell viability under 5 and 30 mM glucose contents. HCQ significantly decreased the total levels of nitric oxide (NO), malondialdehyde (MDA), and reactive oxygen species (ROS) in both sets of environments. Additionally, inhibitory effects were observed on cell migration after exposure to HCQ (P < 0.001). Anti-angiogenic activity of HCQ was confirmed by the reduction of tube areas under a normal or surplus amount of glucose (P < 0.001). Conclusion: In overall, results suggest that HCQ changes the oxidative/nitrosative status of HUVECs both in 5 and 30 mM conditions. HCQ is able to reduce migration and angiogenic activity of HUVECs irrespective of the glucose content.
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spelling pubmed-58015332018-02-12 Effect of hydroxychloroquine on oxidative/nitrosative status and angiogenesis in endothelial cells under high glucose condition Rezabakhsh, Aysa Montazersaheb, Soheila Nabat, Elahe Hassanpour, Mehdi Montaseri, Azadeh Malekinejad, Hassan Movassaghpour, Ali Akbar Rahbarghazi, Reza Garjani, Alireza Bioimpacts Original Research [Image: see text] Introduction: Under the diabetic condition, sustained production of oxidative/nitrosative stress results in irreversible vascular injuries. A great number of diabetic pathologies, such as inefficient or aberrant neo-angiogenesis emerge following chronic hyperglycemic condition. Lack of enough data exists regarding hydroxychloroquine (HCQ) contribution on angiogenesis during diabetes mellitus. Methods: To better address whether HCQ could blunt or exacerbate oxidative status and angiogenesis under high glucose condition (HCG), human umbilical vein endothelial cells (HUVECs) were exposed to 30 µM HCQ in combination with 30 mM glucose over a course of 72 hours. Viability was measured was evaluated by MTT assay. We used Griess method and TBARS assay to monitor changes in the levels of NO and MDA followed by flow cytometric analysis of ROS using DCFDA. To show the impact of HCQ on cell motility and in vitro angiogenic properties, we exploited routine scratch test and in vitro tubulogenesis, respectively. Results: Our data showed that HCQ diminished cell viability under 5 and 30 mM glucose contents. HCQ significantly decreased the total levels of nitric oxide (NO), malondialdehyde (MDA), and reactive oxygen species (ROS) in both sets of environments. Additionally, inhibitory effects were observed on cell migration after exposure to HCQ (P < 0.001). Anti-angiogenic activity of HCQ was confirmed by the reduction of tube areas under a normal or surplus amount of glucose (P < 0.001). Conclusion: In overall, results suggest that HCQ changes the oxidative/nitrosative status of HUVECs both in 5 and 30 mM conditions. HCQ is able to reduce migration and angiogenic activity of HUVECs irrespective of the glucose content. Tabriz University of Medical Sciences 2017 2017-08-23 /pmc/articles/PMC5801533/ /pubmed/29435429 http://dx.doi.org/10.15171/bi.2017.26 Text en © 2017 The Author(s) This work is published by BioImpacts as an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/). Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Research
Rezabakhsh, Aysa
Montazersaheb, Soheila
Nabat, Elahe
Hassanpour, Mehdi
Montaseri, Azadeh
Malekinejad, Hassan
Movassaghpour, Ali Akbar
Rahbarghazi, Reza
Garjani, Alireza
Effect of hydroxychloroquine on oxidative/nitrosative status and angiogenesis in endothelial cells under high glucose condition
title Effect of hydroxychloroquine on oxidative/nitrosative status and angiogenesis in endothelial cells under high glucose condition
title_full Effect of hydroxychloroquine on oxidative/nitrosative status and angiogenesis in endothelial cells under high glucose condition
title_fullStr Effect of hydroxychloroquine on oxidative/nitrosative status and angiogenesis in endothelial cells under high glucose condition
title_full_unstemmed Effect of hydroxychloroquine on oxidative/nitrosative status and angiogenesis in endothelial cells under high glucose condition
title_short Effect of hydroxychloroquine on oxidative/nitrosative status and angiogenesis in endothelial cells under high glucose condition
title_sort effect of hydroxychloroquine on oxidative/nitrosative status and angiogenesis in endothelial cells under high glucose condition
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801533/
https://www.ncbi.nlm.nih.gov/pubmed/29435429
http://dx.doi.org/10.15171/bi.2017.26
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