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Plant vigour QTLs co-map with an earlier reported QTL hotspot for drought tolerance while water saving QTLs map in other regions of the chickpea genome

BACKGROUND: Terminal drought stress leads to substantial annual yield losses in chickpea (Cicer arietinum L.). Adaptation to water limitation is a matter of matching water supply to water demand by the crop. Therefore, harnessing the genetics of traits contributing to plant water use, i.e. transpira...

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Autores principales: Sivasakthi, Kaliamoorthy, Thudi, Mahendar, Tharanya, Murugesan, Kale, Sandip M., Kholová, Jana, Halime, Mahamat Hissene, Jaganathan, Deepa, Baddam, Rekha, Thirunalasundari, Thiyagarajan, Gaur, Pooran M., Varshney, Rajeev K., Vadez, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801699/
https://www.ncbi.nlm.nih.gov/pubmed/29409451
http://dx.doi.org/10.1186/s12870-018-1245-1
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author Sivasakthi, Kaliamoorthy
Thudi, Mahendar
Tharanya, Murugesan
Kale, Sandip M.
Kholová, Jana
Halime, Mahamat Hissene
Jaganathan, Deepa
Baddam, Rekha
Thirunalasundari, Thiyagarajan
Gaur, Pooran M.
Varshney, Rajeev K.
Vadez, Vincent
author_facet Sivasakthi, Kaliamoorthy
Thudi, Mahendar
Tharanya, Murugesan
Kale, Sandip M.
Kholová, Jana
Halime, Mahamat Hissene
Jaganathan, Deepa
Baddam, Rekha
Thirunalasundari, Thiyagarajan
Gaur, Pooran M.
Varshney, Rajeev K.
Vadez, Vincent
author_sort Sivasakthi, Kaliamoorthy
collection PubMed
description BACKGROUND: Terminal drought stress leads to substantial annual yield losses in chickpea (Cicer arietinum L.). Adaptation to water limitation is a matter of matching water supply to water demand by the crop. Therefore, harnessing the genetics of traits contributing to plant water use, i.e. transpiration rate and canopy development dynamics, is important to design crop ideotypes suited to a varying range of water limited environments. With an aim of identifying genomic regions for plant vigour (growth and canopy size) and canopy conductance traits, 232 recombinant inbred lines derived from a cross between ICC 4958 and ICC 1882, were phenotyped at vegetative stage under well-watered conditions using a high throughput phenotyping platform (LeasyScan). RESULTS: Twenty one major quantitative trait loci (M-QTLs) were identified for plant vigour and canopy conductance traits using an ultra-high density bin map. Plant vigour traits had 13 M-QTLs on CaLG04, with favourable alleles from high vigour parent ICC 4958. Most of them co-mapped with a previously fine mapped major drought tolerance “QTL-hotspot” region on CaLG04. One M-QTL was found for canopy conductance on CaLG03 with the ultra-high density bin map. Comparative analysis of the QTLs found across different density genetic maps revealed that QTL size reduced considerably and % of phenotypic variation increased as marker density increased. CONCLUSION: Earlier reported drought tolerance hotspot is a vigour locus. The fact that canopy conductance traits, i.e. the other important determinant of plant water use, mapped on CaLG03 provides an opportunity to manipulate these loci to tailor recombinants having low/high transpiration rate and plant vigour, fitted to specific drought stress scenarios in chickpea. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1245-1) contains supplementary material, which is available to authorized users.
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spelling pubmed-58016992018-02-14 Plant vigour QTLs co-map with an earlier reported QTL hotspot for drought tolerance while water saving QTLs map in other regions of the chickpea genome Sivasakthi, Kaliamoorthy Thudi, Mahendar Tharanya, Murugesan Kale, Sandip M. Kholová, Jana Halime, Mahamat Hissene Jaganathan, Deepa Baddam, Rekha Thirunalasundari, Thiyagarajan Gaur, Pooran M. Varshney, Rajeev K. Vadez, Vincent BMC Plant Biol Research Article BACKGROUND: Terminal drought stress leads to substantial annual yield losses in chickpea (Cicer arietinum L.). Adaptation to water limitation is a matter of matching water supply to water demand by the crop. Therefore, harnessing the genetics of traits contributing to plant water use, i.e. transpiration rate and canopy development dynamics, is important to design crop ideotypes suited to a varying range of water limited environments. With an aim of identifying genomic regions for plant vigour (growth and canopy size) and canopy conductance traits, 232 recombinant inbred lines derived from a cross between ICC 4958 and ICC 1882, were phenotyped at vegetative stage under well-watered conditions using a high throughput phenotyping platform (LeasyScan). RESULTS: Twenty one major quantitative trait loci (M-QTLs) were identified for plant vigour and canopy conductance traits using an ultra-high density bin map. Plant vigour traits had 13 M-QTLs on CaLG04, with favourable alleles from high vigour parent ICC 4958. Most of them co-mapped with a previously fine mapped major drought tolerance “QTL-hotspot” region on CaLG04. One M-QTL was found for canopy conductance on CaLG03 with the ultra-high density bin map. Comparative analysis of the QTLs found across different density genetic maps revealed that QTL size reduced considerably and % of phenotypic variation increased as marker density increased. CONCLUSION: Earlier reported drought tolerance hotspot is a vigour locus. The fact that canopy conductance traits, i.e. the other important determinant of plant water use, mapped on CaLG03 provides an opportunity to manipulate these loci to tailor recombinants having low/high transpiration rate and plant vigour, fitted to specific drought stress scenarios in chickpea. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1245-1) contains supplementary material, which is available to authorized users. BioMed Central 2018-02-06 /pmc/articles/PMC5801699/ /pubmed/29409451 http://dx.doi.org/10.1186/s12870-018-1245-1 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Sivasakthi, Kaliamoorthy
Thudi, Mahendar
Tharanya, Murugesan
Kale, Sandip M.
Kholová, Jana
Halime, Mahamat Hissene
Jaganathan, Deepa
Baddam, Rekha
Thirunalasundari, Thiyagarajan
Gaur, Pooran M.
Varshney, Rajeev K.
Vadez, Vincent
Plant vigour QTLs co-map with an earlier reported QTL hotspot for drought tolerance while water saving QTLs map in other regions of the chickpea genome
title Plant vigour QTLs co-map with an earlier reported QTL hotspot for drought tolerance while water saving QTLs map in other regions of the chickpea genome
title_full Plant vigour QTLs co-map with an earlier reported QTL hotspot for drought tolerance while water saving QTLs map in other regions of the chickpea genome
title_fullStr Plant vigour QTLs co-map with an earlier reported QTL hotspot for drought tolerance while water saving QTLs map in other regions of the chickpea genome
title_full_unstemmed Plant vigour QTLs co-map with an earlier reported QTL hotspot for drought tolerance while water saving QTLs map in other regions of the chickpea genome
title_short Plant vigour QTLs co-map with an earlier reported QTL hotspot for drought tolerance while water saving QTLs map in other regions of the chickpea genome
title_sort plant vigour qtls co-map with an earlier reported qtl hotspot for drought tolerance while water saving qtls map in other regions of the chickpea genome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801699/
https://www.ncbi.nlm.nih.gov/pubmed/29409451
http://dx.doi.org/10.1186/s12870-018-1245-1
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