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Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells

Diphyllin is a natural component of traditional Chinese medicine, which effectively inhibits V-ATPase activity and affects the progression of cancer. However, few studies have been conducted on esophageal cancer, and the mechanisms remain to be elucidated. The present study revealedthat diphyllin in...

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Autores principales: Chen, Haijiao, Liu, Pengfei, Zhang, Ting, Gao, Yi, Zhang, Yingdi, Shen, Xiuyun, Li, Xiao, Shen, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802041/
https://www.ncbi.nlm.nih.gov/pubmed/29328465
http://dx.doi.org/10.3892/or.2018.6191
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author Chen, Haijiao
Liu, Pengfei
Zhang, Ting
Gao, Yi
Zhang, Yingdi
Shen, Xiuyun
Li, Xiao
Shen, Weidong
author_facet Chen, Haijiao
Liu, Pengfei
Zhang, Ting
Gao, Yi
Zhang, Yingdi
Shen, Xiuyun
Li, Xiao
Shen, Weidong
author_sort Chen, Haijiao
collection PubMed
description Diphyllin is a natural component of traditional Chinese medicine, which effectively inhibits V-ATPase activity and affects the progression of cancer. However, few studies have been conducted on esophageal cancer, and the mechanisms remain to be elucidated. The present study revealedthat diphyllin inhibited proliferation and induced S arrest in esophageal cancer cell lines TE-1 and ECA-109. Further experiments revealed that diphyllin inhibited V-ATPase activity and decreased the mRNA expression of mammalian target of rapamycin complex 1 (mTORC1), hypoxia-inducible factor-1α (HIF-1α), and vascular endothelial growth factor (VEGF). The present study also revealed that diphyllin inhibited proliferation and reduced the formation of new blood vessels. Diphyllin inhibited blood metastasis by regulating the mTORC1/HIF-1α-/VEGF pathway, therefore it could be considered as a new V-ATPase inhibitor to treat esophageal cancer.
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spelling pubmed-58020412018-02-26 Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells Chen, Haijiao Liu, Pengfei Zhang, Ting Gao, Yi Zhang, Yingdi Shen, Xiuyun Li, Xiao Shen, Weidong Oncol Rep Articles Diphyllin is a natural component of traditional Chinese medicine, which effectively inhibits V-ATPase activity and affects the progression of cancer. However, few studies have been conducted on esophageal cancer, and the mechanisms remain to be elucidated. The present study revealedthat diphyllin inhibited proliferation and induced S arrest in esophageal cancer cell lines TE-1 and ECA-109. Further experiments revealed that diphyllin inhibited V-ATPase activity and decreased the mRNA expression of mammalian target of rapamycin complex 1 (mTORC1), hypoxia-inducible factor-1α (HIF-1α), and vascular endothelial growth factor (VEGF). The present study also revealed that diphyllin inhibited proliferation and reduced the formation of new blood vessels. Diphyllin inhibited blood metastasis by regulating the mTORC1/HIF-1α-/VEGF pathway, therefore it could be considered as a new V-ATPase inhibitor to treat esophageal cancer. D.A. Spandidos 2018-03 2018-01-04 /pmc/articles/PMC5802041/ /pubmed/29328465 http://dx.doi.org/10.3892/or.2018.6191 Text en Copyright: © Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Chen, Haijiao
Liu, Pengfei
Zhang, Ting
Gao, Yi
Zhang, Yingdi
Shen, Xiuyun
Li, Xiao
Shen, Weidong
Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells
title Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells
title_full Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells
title_fullStr Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells
title_full_unstemmed Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells
title_short Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells
title_sort effects of diphyllin as a novel v-atpase inhibitor on te-1 and eca-109 cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802041/
https://www.ncbi.nlm.nih.gov/pubmed/29328465
http://dx.doi.org/10.3892/or.2018.6191
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