Cargando…
CircDOCK1 suppresses cell apoptosis via inhibition of miR-196a-5p by targeting BIRC3 in OSCC
Oral squamous cell carcinoma (OSCC) is the most frequent oral cancer in the world, accounting for more than 90% of all oral cancer diagnosis. Circular RNAs (circRNAs) are large types of non-coding RNAs, demonstrating a great capacity of regulating the expression of genes. However, most of the functi...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802043/ https://www.ncbi.nlm.nih.gov/pubmed/29286141 http://dx.doi.org/10.3892/or.2017.6174 |
_version_ | 1783298463310544896 |
---|---|
author | Wang, Liping Wei, Yongxiang Yan, Yongyong Wang, Haiyan Yang, Jiantin Zheng, Zhichao Zha, Jun Bo, Peng Tang, Yinghua Guo, Xueqi Chen, Weihong Zhu, Xinxin Ge, Linhu |
author_facet | Wang, Liping Wei, Yongxiang Yan, Yongyong Wang, Haiyan Yang, Jiantin Zheng, Zhichao Zha, Jun Bo, Peng Tang, Yinghua Guo, Xueqi Chen, Weihong Zhu, Xinxin Ge, Linhu |
author_sort | Wang, Liping |
collection | PubMed |
description | Oral squamous cell carcinoma (OSCC) is the most frequent oral cancer in the world, accounting for more than 90% of all oral cancer diagnosis. Circular RNAs (circRNAs) are large types of non-coding RNAs, demonstrating a great capacity of regulating the expression of genes. However, most of the functions of circRNAs are still unknown. Recent research revealed that circRNAs could serve as a miRNA-sponge, consequently regulating the expression of target genes indirectly, including oncogenes. In this study, we built an apoptotic model with TNF-α, and then we confirmed a circRNA associated with the apoptosis of OSCC cells, circDOCK1 by comparing the expression profile of circRNAs in an apoptotic model with that in untreated OSCC cells. We ascertained the presence of circDOCK1 with qRT-PCR and circRNA sequencing. The knockdown of the expression of circDOCK1 led to the increase of apoptosis. Utilizing multiple bioinformatics methods, we predicted the interactions among circRNAs, miRNAs and genes, and built the circDOCK1/miR-196a-5p/BIRC3 axis. Both the silencing of circDOCK1 with small interfering RNA and the upregulation of the expression of miR-196a-5p with mimics led OSCC cells to increase apoptosis and decrease BIRC3 formation. We further confirmed this outcome by comparing the expression of circDOCK1, miR-196a-5p and BIRC3 in oral squamous carcinoma tissue with those in para-carcinoma tissue, and examining the expression profile of circRNAs in oral squamous carcinoma tissue and para-carcinoma tissue with microarray. Our results demonstrated that circDOCK1 regulated BIRC3 expression by functioning as a competing endogenous RNA (ceRNA) and participated in the process of OSCC apoptosis. Thus, we propose that circDOCK1 could represent a novel potential biomarker and therapeutic target of OSCC. |
format | Online Article Text |
id | pubmed-5802043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-58020432018-02-26 CircDOCK1 suppresses cell apoptosis via inhibition of miR-196a-5p by targeting BIRC3 in OSCC Wang, Liping Wei, Yongxiang Yan, Yongyong Wang, Haiyan Yang, Jiantin Zheng, Zhichao Zha, Jun Bo, Peng Tang, Yinghua Guo, Xueqi Chen, Weihong Zhu, Xinxin Ge, Linhu Oncol Rep Articles Oral squamous cell carcinoma (OSCC) is the most frequent oral cancer in the world, accounting for more than 90% of all oral cancer diagnosis. Circular RNAs (circRNAs) are large types of non-coding RNAs, demonstrating a great capacity of regulating the expression of genes. However, most of the functions of circRNAs are still unknown. Recent research revealed that circRNAs could serve as a miRNA-sponge, consequently regulating the expression of target genes indirectly, including oncogenes. In this study, we built an apoptotic model with TNF-α, and then we confirmed a circRNA associated with the apoptosis of OSCC cells, circDOCK1 by comparing the expression profile of circRNAs in an apoptotic model with that in untreated OSCC cells. We ascertained the presence of circDOCK1 with qRT-PCR and circRNA sequencing. The knockdown of the expression of circDOCK1 led to the increase of apoptosis. Utilizing multiple bioinformatics methods, we predicted the interactions among circRNAs, miRNAs and genes, and built the circDOCK1/miR-196a-5p/BIRC3 axis. Both the silencing of circDOCK1 with small interfering RNA and the upregulation of the expression of miR-196a-5p with mimics led OSCC cells to increase apoptosis and decrease BIRC3 formation. We further confirmed this outcome by comparing the expression of circDOCK1, miR-196a-5p and BIRC3 in oral squamous carcinoma tissue with those in para-carcinoma tissue, and examining the expression profile of circRNAs in oral squamous carcinoma tissue and para-carcinoma tissue with microarray. Our results demonstrated that circDOCK1 regulated BIRC3 expression by functioning as a competing endogenous RNA (ceRNA) and participated in the process of OSCC apoptosis. Thus, we propose that circDOCK1 could represent a novel potential biomarker and therapeutic target of OSCC. D.A. Spandidos 2018-03 2017-12-28 /pmc/articles/PMC5802043/ /pubmed/29286141 http://dx.doi.org/10.3892/or.2017.6174 Text en Copyright: © Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wang, Liping Wei, Yongxiang Yan, Yongyong Wang, Haiyan Yang, Jiantin Zheng, Zhichao Zha, Jun Bo, Peng Tang, Yinghua Guo, Xueqi Chen, Weihong Zhu, Xinxin Ge, Linhu CircDOCK1 suppresses cell apoptosis via inhibition of miR-196a-5p by targeting BIRC3 in OSCC |
title | CircDOCK1 suppresses cell apoptosis via inhibition of miR-196a-5p by targeting BIRC3 in OSCC |
title_full | CircDOCK1 suppresses cell apoptosis via inhibition of miR-196a-5p by targeting BIRC3 in OSCC |
title_fullStr | CircDOCK1 suppresses cell apoptosis via inhibition of miR-196a-5p by targeting BIRC3 in OSCC |
title_full_unstemmed | CircDOCK1 suppresses cell apoptosis via inhibition of miR-196a-5p by targeting BIRC3 in OSCC |
title_short | CircDOCK1 suppresses cell apoptosis via inhibition of miR-196a-5p by targeting BIRC3 in OSCC |
title_sort | circdock1 suppresses cell apoptosis via inhibition of mir-196a-5p by targeting birc3 in oscc |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802043/ https://www.ncbi.nlm.nih.gov/pubmed/29286141 http://dx.doi.org/10.3892/or.2017.6174 |
work_keys_str_mv | AT wangliping circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc AT weiyongxiang circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc AT yanyongyong circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc AT wanghaiyan circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc AT yangjiantin circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc AT zhengzhichao circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc AT zhajun circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc AT bopeng circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc AT tangyinghua circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc AT guoxueqi circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc AT chenweihong circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc AT zhuxinxin circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc AT gelinhu circdock1suppressescellapoptosisviainhibitionofmir196a5pbytargetingbirc3inoscc |