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Crucial role of OX40/OX40L signaling in a murine model of asthma
The aim of the present study was to explore the roles of OX40/OX40 ligand (OX40L) signaling and OX40(+) T cells in ovalbumin (OVA)-induced mouse asthma model. Asthma was induced by OVA exposure and subsequent co-treatment with OX40L protein, neutralizing anti-OX40L blocking antibody, OX40(+) T cells...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802192/ https://www.ncbi.nlm.nih.gov/pubmed/29344664 http://dx.doi.org/10.3892/mmr.2018.8453 |
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author | Lei, Wei Zeng, Daxiong Liu, Gaoqin Zhu, Yehan Wang, Jiajia Wu, Hongya Jiang, Junhong Huang, Jianan |
author_facet | Lei, Wei Zeng, Daxiong Liu, Gaoqin Zhu, Yehan Wang, Jiajia Wu, Hongya Jiang, Junhong Huang, Jianan |
author_sort | Lei, Wei |
collection | PubMed |
description | The aim of the present study was to explore the roles of OX40/OX40 ligand (OX40L) signaling and OX40(+) T cells in ovalbumin (OVA)-induced mouse asthma model. Asthma was induced by OVA exposure and subsequent co-treatment with OX40L protein, neutralizing anti-OX40L blocking antibody, OX40(+) T cells or PBS. The protein expression levels of interleukin (IL)-4, IL-6, IL-13, IL-17, tumor necrosis factor (TNF)-α and interferon (IFN)-γ in bronchoalveolar lavage fluid (BALF) were examined using murine cytokine-specific ELISA. Eosinophil accumulation as well as proliferation and apoptosis of T cells in BALF were detected by Cell Counting kit-8 and flow cytometric assays. Expression of the apoptosis-related protein cleaved caspase-3 was examined in OX40(+) T cells using western blot assay. Flow cytometric analysis revealed that OVA-treated mice that were co-treated with OX40L or OX40(+) T cells exhibited higher eosinophil infiltration compared with control mice treated only with OVA, whereas neutralizing anti-OX40L blocking antibody inhibited eosinophil infiltration. ELISA assays demonstrated that the expression of IL-4, IL-6, IL-13, IL-17, TNF-α and IFN-γ in BALF in OX40L-treated and OX40(+) T cell-treated mice was increased compared with expression levels in control mice. Treatment with OX40L protein effectively reduced apoptosis of T cells and the expression of cleaved caspase-3 in T cells. OX40L-treated and OX40(+) T cell-treated mice exhibited increased asthma through OX40/OX40L signaling, which probably promoted inflammatory factor expression, eosinophil infiltration and T cell proliferation. |
format | Online Article Text |
id | pubmed-5802192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-58021922018-02-26 Crucial role of OX40/OX40L signaling in a murine model of asthma Lei, Wei Zeng, Daxiong Liu, Gaoqin Zhu, Yehan Wang, Jiajia Wu, Hongya Jiang, Junhong Huang, Jianan Mol Med Rep Articles The aim of the present study was to explore the roles of OX40/OX40 ligand (OX40L) signaling and OX40(+) T cells in ovalbumin (OVA)-induced mouse asthma model. Asthma was induced by OVA exposure and subsequent co-treatment with OX40L protein, neutralizing anti-OX40L blocking antibody, OX40(+) T cells or PBS. The protein expression levels of interleukin (IL)-4, IL-6, IL-13, IL-17, tumor necrosis factor (TNF)-α and interferon (IFN)-γ in bronchoalveolar lavage fluid (BALF) were examined using murine cytokine-specific ELISA. Eosinophil accumulation as well as proliferation and apoptosis of T cells in BALF were detected by Cell Counting kit-8 and flow cytometric assays. Expression of the apoptosis-related protein cleaved caspase-3 was examined in OX40(+) T cells using western blot assay. Flow cytometric analysis revealed that OVA-treated mice that were co-treated with OX40L or OX40(+) T cells exhibited higher eosinophil infiltration compared with control mice treated only with OVA, whereas neutralizing anti-OX40L blocking antibody inhibited eosinophil infiltration. ELISA assays demonstrated that the expression of IL-4, IL-6, IL-13, IL-17, TNF-α and IFN-γ in BALF in OX40L-treated and OX40(+) T cell-treated mice was increased compared with expression levels in control mice. Treatment with OX40L protein effectively reduced apoptosis of T cells and the expression of cleaved caspase-3 in T cells. OX40L-treated and OX40(+) T cell-treated mice exhibited increased asthma through OX40/OX40L signaling, which probably promoted inflammatory factor expression, eosinophil infiltration and T cell proliferation. D.A. Spandidos 2018-03 2018-01-18 /pmc/articles/PMC5802192/ /pubmed/29344664 http://dx.doi.org/10.3892/mmr.2018.8453 Text en Copyright: © Lei et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Lei, Wei Zeng, Daxiong Liu, Gaoqin Zhu, Yehan Wang, Jiajia Wu, Hongya Jiang, Junhong Huang, Jianan Crucial role of OX40/OX40L signaling in a murine model of asthma |
title | Crucial role of OX40/OX40L signaling in a murine model of asthma |
title_full | Crucial role of OX40/OX40L signaling in a murine model of asthma |
title_fullStr | Crucial role of OX40/OX40L signaling in a murine model of asthma |
title_full_unstemmed | Crucial role of OX40/OX40L signaling in a murine model of asthma |
title_short | Crucial role of OX40/OX40L signaling in a murine model of asthma |
title_sort | crucial role of ox40/ox40l signaling in a murine model of asthma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802192/ https://www.ncbi.nlm.nih.gov/pubmed/29344664 http://dx.doi.org/10.3892/mmr.2018.8453 |
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