Cargando…

Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury

In the present study, the effects of hyaluronic acid (HA) combined with chitosan conduit on peripheral nerve scarring and regeneration were investigated in a rat model of peripheral nerve crush injury. A total of 60 Sprague-Dawley rats were randomly distributed into four groups (15 rats in each grou...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Runxin, Liu, Huawei, Huang, Haitao, Bi, Wenting, Yan, Rongzeng, Tan, Xinying, Wen, Weisheng, Wang, Chao, Song, Wenling, Zhang, Yanhua, Zhang, Feng, Hu, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802209/
https://www.ncbi.nlm.nih.gov/pubmed/29328458
http://dx.doi.org/10.3892/mmr.2018.8388
_version_ 1783298501212372992
author Li, Runxin
Liu, Huawei
Huang, Haitao
Bi, Wenting
Yan, Rongzeng
Tan, Xinying
Wen, Weisheng
Wang, Chao
Song, Wenling
Zhang, Yanhua
Zhang, Feng
Hu, Min
author_facet Li, Runxin
Liu, Huawei
Huang, Haitao
Bi, Wenting
Yan, Rongzeng
Tan, Xinying
Wen, Weisheng
Wang, Chao
Song, Wenling
Zhang, Yanhua
Zhang, Feng
Hu, Min
author_sort Li, Runxin
collection PubMed
description In the present study, the effects of hyaluronic acid (HA) combined with chitosan conduit on peripheral nerve scarring and regeneration were investigated in a rat model of peripheral nerve crush injury. A total of 60 Sprague-Dawley rats were randomly distributed into four groups (15 rats in each group), in which the nerve was either not treated (control group) or treated with chitosan conduit, hyaluronic acid, or chitosan conduit coupled with hyaluronic acid following clamp injury to the sciatic nerve. The surgical sites were evaluated by assessing the sciatic functional index, the degree of scar adhesions, the numbers of myelinated nerve fibers, the average diameter of myelinated nerve fibers and the myelin sheath thickness. Larger epineurial scar thickness was observed in the control groups compared with the treatment groups at 4, 8 and 12 weeks following surgery. There was no significant difference in scar adhesion among the four groups at 4 weeks following surgery. However, animals receiving chitosan coupled with HA demonstrated better neural recovery, as measured by reduced nerve adherence to surrounding tissues, less scar adhesion, increased number of axons, nerve fiber diameter and myelin thickness. In conclusion, the application of chitosan conduit combined with HA, to a certain extent, inhibited sciatic nerve extraneural scaring and adhesion, and promoted neural regeneration and recovery.
format Online
Article
Text
id pubmed-5802209
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-58022092018-02-26 Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury Li, Runxin Liu, Huawei Huang, Haitao Bi, Wenting Yan, Rongzeng Tan, Xinying Wen, Weisheng Wang, Chao Song, Wenling Zhang, Yanhua Zhang, Feng Hu, Min Mol Med Rep Articles In the present study, the effects of hyaluronic acid (HA) combined with chitosan conduit on peripheral nerve scarring and regeneration were investigated in a rat model of peripheral nerve crush injury. A total of 60 Sprague-Dawley rats were randomly distributed into four groups (15 rats in each group), in which the nerve was either not treated (control group) or treated with chitosan conduit, hyaluronic acid, or chitosan conduit coupled with hyaluronic acid following clamp injury to the sciatic nerve. The surgical sites were evaluated by assessing the sciatic functional index, the degree of scar adhesions, the numbers of myelinated nerve fibers, the average diameter of myelinated nerve fibers and the myelin sheath thickness. Larger epineurial scar thickness was observed in the control groups compared with the treatment groups at 4, 8 and 12 weeks following surgery. There was no significant difference in scar adhesion among the four groups at 4 weeks following surgery. However, animals receiving chitosan coupled with HA demonstrated better neural recovery, as measured by reduced nerve adherence to surrounding tissues, less scar adhesion, increased number of axons, nerve fiber diameter and myelin thickness. In conclusion, the application of chitosan conduit combined with HA, to a certain extent, inhibited sciatic nerve extraneural scaring and adhesion, and promoted neural regeneration and recovery. D.A. Spandidos 2018-03 2018-01-08 /pmc/articles/PMC5802209/ /pubmed/29328458 http://dx.doi.org/10.3892/mmr.2018.8388 Text en Copyright: © Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Li, Runxin
Liu, Huawei
Huang, Haitao
Bi, Wenting
Yan, Rongzeng
Tan, Xinying
Wen, Weisheng
Wang, Chao
Song, Wenling
Zhang, Yanhua
Zhang, Feng
Hu, Min
Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury
title Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury
title_full Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury
title_fullStr Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury
title_full_unstemmed Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury
title_short Chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury
title_sort chitosan conduit combined with hyaluronic acid prevent sciatic nerve scar in a rat model of peripheral nerve crush injury
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802209/
https://www.ncbi.nlm.nih.gov/pubmed/29328458
http://dx.doi.org/10.3892/mmr.2018.8388
work_keys_str_mv AT lirunxin chitosanconduitcombinedwithhyaluronicacidpreventsciaticnervescarinaratmodelofperipheralnervecrushinjury
AT liuhuawei chitosanconduitcombinedwithhyaluronicacidpreventsciaticnervescarinaratmodelofperipheralnervecrushinjury
AT huanghaitao chitosanconduitcombinedwithhyaluronicacidpreventsciaticnervescarinaratmodelofperipheralnervecrushinjury
AT biwenting chitosanconduitcombinedwithhyaluronicacidpreventsciaticnervescarinaratmodelofperipheralnervecrushinjury
AT yanrongzeng chitosanconduitcombinedwithhyaluronicacidpreventsciaticnervescarinaratmodelofperipheralnervecrushinjury
AT tanxinying chitosanconduitcombinedwithhyaluronicacidpreventsciaticnervescarinaratmodelofperipheralnervecrushinjury
AT wenweisheng chitosanconduitcombinedwithhyaluronicacidpreventsciaticnervescarinaratmodelofperipheralnervecrushinjury
AT wangchao chitosanconduitcombinedwithhyaluronicacidpreventsciaticnervescarinaratmodelofperipheralnervecrushinjury
AT songwenling chitosanconduitcombinedwithhyaluronicacidpreventsciaticnervescarinaratmodelofperipheralnervecrushinjury
AT zhangyanhua chitosanconduitcombinedwithhyaluronicacidpreventsciaticnervescarinaratmodelofperipheralnervecrushinjury
AT zhangfeng chitosanconduitcombinedwithhyaluronicacidpreventsciaticnervescarinaratmodelofperipheralnervecrushinjury
AT humin chitosanconduitcombinedwithhyaluronicacidpreventsciaticnervescarinaratmodelofperipheralnervecrushinjury