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Comparing autotransporter β-domain configurations for their capacity to secrete heterologous proteins to the cell surface
Monomeric autotransporters have been extensively used for export of recombinant proteins to the cell surface of Gram-negative bacteria. A bottleneck in the biosynthesis of such constructs is the passage of the outer membrane, which is facilitated by the β-domain at the C terminus of an autotransport...
Autores principales: | Jong, Wouter S. P., Schillemans, Maaike, ten Hagen-Jongman, Corinne M., Luirink, Joen, van Ulsen, Peter |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802855/ https://www.ncbi.nlm.nih.gov/pubmed/29415042 http://dx.doi.org/10.1371/journal.pone.0191622 |
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