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Can we induce spermatogenesis in the domestic cat using an in vitro tissue culture approach?
The reduced number of animals in most wild felid populations implies a loss of genetic diversity. The death of juveniles, prior to the production of mature sperm, represents a loss of potential genetic contribution to future populations. Since 2011 mouse testicular organ culture has introduced an al...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802888/ https://www.ncbi.nlm.nih.gov/pubmed/29414992 http://dx.doi.org/10.1371/journal.pone.0191912 |
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author | Silva, Andreia F. Escada-Rebelo, Sara Amaral, Sandra Tavares, Renata S. Schlatt, Stefan Ramalho-Santos, João Mota, Paula C. |
author_facet | Silva, Andreia F. Escada-Rebelo, Sara Amaral, Sandra Tavares, Renata S. Schlatt, Stefan Ramalho-Santos, João Mota, Paula C. |
author_sort | Silva, Andreia F. |
collection | PubMed |
description | The reduced number of animals in most wild felid populations implies a loss of genetic diversity. The death of juveniles, prior to the production of mature sperm, represents a loss of potential genetic contribution to future populations. Since 2011 mouse testicular organ culture has introduced an alternative mechanism to produce sperm in vitro from immature tissue. However, extension of this technology to other species has remained limited. We have used the domestic cat (Felis catus) as a model for wild felids to investigate spermatogenesis initiation and regulation, with the mouse serving as a control species. Testicular tissue fragments were cultured in control medium or medium supplemented with knockout serum replacement (KSR), AlbuMax, beta-estradiol or AlbuMax plus beta-estradiol. Contrary to expectations, and unlike results obtained in mouse controls, no germ cell differentiation could be detected. The only germ cells observed after six weeks of culture were spermatogonia regardless of the initial stage of tubule development in the donor tissue. Moreover, the number of spermatogonia decreased with time in culture in all media tested, especially in the medium supplemented with KSR, while AlbuMax had a slight protective effect. The combination of AlbuMax and beta-estradiol led to an increase in the area occupied by seminiferous tubules, and thus to an increase in total number of spermatogonial cells. Considering all the media combinations tested the stimulus for felid germ cell differentiation in this type of system seems to be different from the mouse. Studies using other triggers of differentiation and tissue survival factors should be performed to pursue this technology for the genetic diversity preservation in wild felids. |
format | Online Article Text |
id | pubmed-5802888 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58028882018-02-23 Can we induce spermatogenesis in the domestic cat using an in vitro tissue culture approach? Silva, Andreia F. Escada-Rebelo, Sara Amaral, Sandra Tavares, Renata S. Schlatt, Stefan Ramalho-Santos, João Mota, Paula C. PLoS One Research Article The reduced number of animals in most wild felid populations implies a loss of genetic diversity. The death of juveniles, prior to the production of mature sperm, represents a loss of potential genetic contribution to future populations. Since 2011 mouse testicular organ culture has introduced an alternative mechanism to produce sperm in vitro from immature tissue. However, extension of this technology to other species has remained limited. We have used the domestic cat (Felis catus) as a model for wild felids to investigate spermatogenesis initiation and regulation, with the mouse serving as a control species. Testicular tissue fragments were cultured in control medium or medium supplemented with knockout serum replacement (KSR), AlbuMax, beta-estradiol or AlbuMax plus beta-estradiol. Contrary to expectations, and unlike results obtained in mouse controls, no germ cell differentiation could be detected. The only germ cells observed after six weeks of culture were spermatogonia regardless of the initial stage of tubule development in the donor tissue. Moreover, the number of spermatogonia decreased with time in culture in all media tested, especially in the medium supplemented with KSR, while AlbuMax had a slight protective effect. The combination of AlbuMax and beta-estradiol led to an increase in the area occupied by seminiferous tubules, and thus to an increase in total number of spermatogonial cells. Considering all the media combinations tested the stimulus for felid germ cell differentiation in this type of system seems to be different from the mouse. Studies using other triggers of differentiation and tissue survival factors should be performed to pursue this technology for the genetic diversity preservation in wild felids. Public Library of Science 2018-02-07 /pmc/articles/PMC5802888/ /pubmed/29414992 http://dx.doi.org/10.1371/journal.pone.0191912 Text en © 2018 Silva et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Silva, Andreia F. Escada-Rebelo, Sara Amaral, Sandra Tavares, Renata S. Schlatt, Stefan Ramalho-Santos, João Mota, Paula C. Can we induce spermatogenesis in the domestic cat using an in vitro tissue culture approach? |
title | Can we induce spermatogenesis in the domestic cat using an in vitro tissue culture approach? |
title_full | Can we induce spermatogenesis in the domestic cat using an in vitro tissue culture approach? |
title_fullStr | Can we induce spermatogenesis in the domestic cat using an in vitro tissue culture approach? |
title_full_unstemmed | Can we induce spermatogenesis in the domestic cat using an in vitro tissue culture approach? |
title_short | Can we induce spermatogenesis in the domestic cat using an in vitro tissue culture approach? |
title_sort | can we induce spermatogenesis in the domestic cat using an in vitro tissue culture approach? |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802888/ https://www.ncbi.nlm.nih.gov/pubmed/29414992 http://dx.doi.org/10.1371/journal.pone.0191912 |
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