Cargando…
Antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice
BACKGROUND: Obesity is associated with gut microbiota dysbiosis, disrupted intestinal barrier and chronic inflammation. Given the high and increasing prevalence of obesity worldwide, anti-obesity treatments that are safe, effective and widely available would be beneficial. We examined whether the me...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5803574/ https://www.ncbi.nlm.nih.gov/pubmed/28630461 http://dx.doi.org/10.1038/ijo.2017.149 |
_version_ | 1783298681340952576 |
---|---|
author | Chang, C-J Lu, C-C Lin, C-S Martel, J Ko, Y-F Ojcius, D M Wu, T-R Tsai, Y-H Yeh, T-S Lu, J-J Lai, H-C Young, J D |
author_facet | Chang, C-J Lu, C-C Lin, C-S Martel, J Ko, Y-F Ojcius, D M Wu, T-R Tsai, Y-H Yeh, T-S Lu, J-J Lai, H-C Young, J D |
author_sort | Chang, C-J |
collection | PubMed |
description | BACKGROUND: Obesity is associated with gut microbiota dysbiosis, disrupted intestinal barrier and chronic inflammation. Given the high and increasing prevalence of obesity worldwide, anti-obesity treatments that are safe, effective and widely available would be beneficial. We examined whether the medicinal mushroom Antrodia cinnamomea may reduce obesity in mice fed with a high-fat diet (HFD). METHODS: Male C57BL/6J mice were fed a HFD for 8 weeks to induce obesity and chronic inflammation. The mice were treated with a water extract of A. cinnamomea (WEAC), and body weight, fat accumulation, inflammation markers, insulin sensitivity and the gut microbiota were monitored. RESULTS: After 8 weeks, the mean body weight of HFD-fed mice was 39.8±1.2 g compared with 35.8±1.3 g for the HFD+1% WEAC group, corresponding to a reduction of 4 g or 10% of body weight (P<0.0001). WEAC supplementation reduced fat accumulation and serum triglycerides in a statistically significant manner in HFD-fed mice. WEAC also reversed the effects of HFD on inflammation markers (interleukin-1β, interleukin-6, tumor necrosis factor-α), insulin resistance and adipokine production (leptin and adiponectin). Notably, WEAC increased the expression of intestinal tight junctions (zonula occludens-1 and occludin) and antimicrobial proteins (Reg3g and lysozyme C) in the small intestine, leading to reduced blood endotoxemia. Finally, WEAC modulated the composition of the gut microbiota, reducing the Firmicutes/Bacteroidetes ratio and increasing the level of Akkermansia muciniphila and other bacterial species associated with anti-inflammatory properties. CONCLUSIONS: Supplementation with A. cinnamomea produces anti-obesogenic, anti-inflammatory and antidiabetic effects in HFD-fed mice by maintaining intestinal integrity and modulating the gut microbiota. |
format | Online Article Text |
id | pubmed-5803574 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-58035742018-02-09 Antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice Chang, C-J Lu, C-C Lin, C-S Martel, J Ko, Y-F Ojcius, D M Wu, T-R Tsai, Y-H Yeh, T-S Lu, J-J Lai, H-C Young, J D Int J Obes (Lond) Original Article BACKGROUND: Obesity is associated with gut microbiota dysbiosis, disrupted intestinal barrier and chronic inflammation. Given the high and increasing prevalence of obesity worldwide, anti-obesity treatments that are safe, effective and widely available would be beneficial. We examined whether the medicinal mushroom Antrodia cinnamomea may reduce obesity in mice fed with a high-fat diet (HFD). METHODS: Male C57BL/6J mice were fed a HFD for 8 weeks to induce obesity and chronic inflammation. The mice were treated with a water extract of A. cinnamomea (WEAC), and body weight, fat accumulation, inflammation markers, insulin sensitivity and the gut microbiota were monitored. RESULTS: After 8 weeks, the mean body weight of HFD-fed mice was 39.8±1.2 g compared with 35.8±1.3 g for the HFD+1% WEAC group, corresponding to a reduction of 4 g or 10% of body weight (P<0.0001). WEAC supplementation reduced fat accumulation and serum triglycerides in a statistically significant manner in HFD-fed mice. WEAC also reversed the effects of HFD on inflammation markers (interleukin-1β, interleukin-6, tumor necrosis factor-α), insulin resistance and adipokine production (leptin and adiponectin). Notably, WEAC increased the expression of intestinal tight junctions (zonula occludens-1 and occludin) and antimicrobial proteins (Reg3g and lysozyme C) in the small intestine, leading to reduced blood endotoxemia. Finally, WEAC modulated the composition of the gut microbiota, reducing the Firmicutes/Bacteroidetes ratio and increasing the level of Akkermansia muciniphila and other bacterial species associated with anti-inflammatory properties. CONCLUSIONS: Supplementation with A. cinnamomea produces anti-obesogenic, anti-inflammatory and antidiabetic effects in HFD-fed mice by maintaining intestinal integrity and modulating the gut microbiota. Nature Publishing Group 2018-02 2017-08-08 /pmc/articles/PMC5803574/ /pubmed/28630461 http://dx.doi.org/10.1038/ijo.2017.149 Text en Copyright © 2018 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/ |
spellingShingle | Original Article Chang, C-J Lu, C-C Lin, C-S Martel, J Ko, Y-F Ojcius, D M Wu, T-R Tsai, Y-H Yeh, T-S Lu, J-J Lai, H-C Young, J D Antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice |
title | Antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice |
title_full | Antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice |
title_fullStr | Antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice |
title_full_unstemmed | Antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice |
title_short | Antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice |
title_sort | antrodia cinnamomea reduces obesity and modulates the gut microbiota in high-fat diet-fed mice |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5803574/ https://www.ncbi.nlm.nih.gov/pubmed/28630461 http://dx.doi.org/10.1038/ijo.2017.149 |
work_keys_str_mv | AT changcj antrodiacinnamomeareducesobesityandmodulatesthegutmicrobiotainhighfatdietfedmice AT lucc antrodiacinnamomeareducesobesityandmodulatesthegutmicrobiotainhighfatdietfedmice AT lincs antrodiacinnamomeareducesobesityandmodulatesthegutmicrobiotainhighfatdietfedmice AT martelj antrodiacinnamomeareducesobesityandmodulatesthegutmicrobiotainhighfatdietfedmice AT koyf antrodiacinnamomeareducesobesityandmodulatesthegutmicrobiotainhighfatdietfedmice AT ojciusdm antrodiacinnamomeareducesobesityandmodulatesthegutmicrobiotainhighfatdietfedmice AT wutr antrodiacinnamomeareducesobesityandmodulatesthegutmicrobiotainhighfatdietfedmice AT tsaiyh antrodiacinnamomeareducesobesityandmodulatesthegutmicrobiotainhighfatdietfedmice AT yehts antrodiacinnamomeareducesobesityandmodulatesthegutmicrobiotainhighfatdietfedmice AT lujj antrodiacinnamomeareducesobesityandmodulatesthegutmicrobiotainhighfatdietfedmice AT laihc antrodiacinnamomeareducesobesityandmodulatesthegutmicrobiotainhighfatdietfedmice AT youngjd antrodiacinnamomeareducesobesityandmodulatesthegutmicrobiotainhighfatdietfedmice |