Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis

OBJECTIVES: This study aimed to provide a rapid, accurate and cost-effective diagnostic real time polymerase chain reaction-high resolution melting curve assay (PCR-HRM) to identify and distinguish between four different mycoplasmas and Acholeplasma laidlawii isolated at cow-level from a single comm...

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Autores principales: Al-Farha, Abd Al-Bar, Petrovski, Kiro, Jozani, Razi, Hoare, Andrew, Hemmatzadeh, Farhid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5804061/
https://www.ncbi.nlm.nih.gov/pubmed/29415764
http://dx.doi.org/10.1186/s13104-018-3223-y
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author Al-Farha, Abd Al-Bar
Petrovski, Kiro
Jozani, Razi
Hoare, Andrew
Hemmatzadeh, Farhid
author_facet Al-Farha, Abd Al-Bar
Petrovski, Kiro
Jozani, Razi
Hoare, Andrew
Hemmatzadeh, Farhid
author_sort Al-Farha, Abd Al-Bar
collection PubMed
description OBJECTIVES: This study aimed to provide a rapid, accurate and cost-effective diagnostic real time polymerase chain reaction-high resolution melting curve assay (PCR-HRM) to identify and distinguish between four different mycoplasmas and Acholeplasma laidlawii isolated at cow-level from a single commercial dairy farm in South Australia. One set of genus-level universal primers was designed targeting the 16S ribosomal RNA gene. RESULTS: Real time PCR-HRM analysis was able to identify and distinguish between five different mollicutes, namely A. laidlawii, M. arginini, M. bovirhinis, M. bovis and uncultured Mycoplasma. Results were confirmed through sequencing. Our developed assay provides rapid and accurate screening for Mycoplasma mastitis detection.
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spelling pubmed-58040612018-02-14 Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis Al-Farha, Abd Al-Bar Petrovski, Kiro Jozani, Razi Hoare, Andrew Hemmatzadeh, Farhid BMC Res Notes Research Note OBJECTIVES: This study aimed to provide a rapid, accurate and cost-effective diagnostic real time polymerase chain reaction-high resolution melting curve assay (PCR-HRM) to identify and distinguish between four different mycoplasmas and Acholeplasma laidlawii isolated at cow-level from a single commercial dairy farm in South Australia. One set of genus-level universal primers was designed targeting the 16S ribosomal RNA gene. RESULTS: Real time PCR-HRM analysis was able to identify and distinguish between five different mollicutes, namely A. laidlawii, M. arginini, M. bovirhinis, M. bovis and uncultured Mycoplasma. Results were confirmed through sequencing. Our developed assay provides rapid and accurate screening for Mycoplasma mastitis detection. BioMed Central 2018-02-07 /pmc/articles/PMC5804061/ /pubmed/29415764 http://dx.doi.org/10.1186/s13104-018-3223-y Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Note
Al-Farha, Abd Al-Bar
Petrovski, Kiro
Jozani, Razi
Hoare, Andrew
Hemmatzadeh, Farhid
Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis
title Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis
title_full Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis
title_fullStr Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis
title_full_unstemmed Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis
title_short Discrimination between some Mycoplasma spp. and Acholeplasma laidlawii in bovine milk using high resolution melting curve analysis
title_sort discrimination between some mycoplasma spp. and acholeplasma laidlawii in bovine milk using high resolution melting curve analysis
topic Research Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5804061/
https://www.ncbi.nlm.nih.gov/pubmed/29415764
http://dx.doi.org/10.1186/s13104-018-3223-y
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