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Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes

Ruminants derived products have a prominent role in diets and economy worldwide; therefore, the capability to control the rumen microbial ecosystem, for ameliorating their quality, is of fundamental importance in the livestock sector. The aim of this study was to evaluate the effect of dietary suppl...

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Autores principales: Buccioni, Arianna, Pallara, Grazia, Pastorelli, Roberta, Bellini, Letizia, Cappucci, Alice, Mannelli, Federica, Minieri, Sara, Roscini, Valentina, Rapaccini, Stefano, Mele, Marcello, Giovannetti, Luciana, Viti, Carlo, Pauselli, Mariano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5804114/
https://www.ncbi.nlm.nih.gov/pubmed/29457028
http://dx.doi.org/10.1155/2017/4969076
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author Buccioni, Arianna
Pallara, Grazia
Pastorelli, Roberta
Bellini, Letizia
Cappucci, Alice
Mannelli, Federica
Minieri, Sara
Roscini, Valentina
Rapaccini, Stefano
Mele, Marcello
Giovannetti, Luciana
Viti, Carlo
Pauselli, Mariano
author_facet Buccioni, Arianna
Pallara, Grazia
Pastorelli, Roberta
Bellini, Letizia
Cappucci, Alice
Mannelli, Federica
Minieri, Sara
Roscini, Valentina
Rapaccini, Stefano
Mele, Marcello
Giovannetti, Luciana
Viti, Carlo
Pauselli, Mariano
author_sort Buccioni, Arianna
collection PubMed
description Ruminants derived products have a prominent role in diets and economy worldwide; therefore, the capability to control the rumen microbial ecosystem, for ameliorating their quality, is of fundamental importance in the livestock sector. The aim of this study was to evaluate the effect of dietary supplementation with chestnut and quebracho tannins on microbial community and fatty acid profile, in the rumen fluid of dairy ewes. Multivariate analysis of PCR-DGGE profiles of rumen microbial communities showed a correlation among the presence of chestnut or quebracho in the diet, the specific Butyrivibrio group DGGE profiles, the increase in 18:3 cis9, cis12, and cis15; 18:2 cis9 and cis12; 18:2 cis9 and trans11; 18:2 trans11 and cis15; and 18:1 trans11 content, and the decrease in 18:0 concentration. Phylogenetic analysis of DGGE band sequences revealed the presence of bacteria representatives related to the genera Hungatella, Ruminococcus, and Eubacterium and unclassified Lachnospiraceae family members, suggesting that these taxa could be affected by tannins presence in the diets. The results of this study showed that tannins from chestnut and quebracho can reduce the biohydrogenation of unsaturated fatty acids through changes in rumen microbial communities.
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spelling pubmed-58041142018-02-18 Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes Buccioni, Arianna Pallara, Grazia Pastorelli, Roberta Bellini, Letizia Cappucci, Alice Mannelli, Federica Minieri, Sara Roscini, Valentina Rapaccini, Stefano Mele, Marcello Giovannetti, Luciana Viti, Carlo Pauselli, Mariano Biomed Res Int Research Article Ruminants derived products have a prominent role in diets and economy worldwide; therefore, the capability to control the rumen microbial ecosystem, for ameliorating their quality, is of fundamental importance in the livestock sector. The aim of this study was to evaluate the effect of dietary supplementation with chestnut and quebracho tannins on microbial community and fatty acid profile, in the rumen fluid of dairy ewes. Multivariate analysis of PCR-DGGE profiles of rumen microbial communities showed a correlation among the presence of chestnut or quebracho in the diet, the specific Butyrivibrio group DGGE profiles, the increase in 18:3 cis9, cis12, and cis15; 18:2 cis9 and cis12; 18:2 cis9 and trans11; 18:2 trans11 and cis15; and 18:1 trans11 content, and the decrease in 18:0 concentration. Phylogenetic analysis of DGGE band sequences revealed the presence of bacteria representatives related to the genera Hungatella, Ruminococcus, and Eubacterium and unclassified Lachnospiraceae family members, suggesting that these taxa could be affected by tannins presence in the diets. The results of this study showed that tannins from chestnut and quebracho can reduce the biohydrogenation of unsaturated fatty acids through changes in rumen microbial communities. Hindawi 2017 2017-12-31 /pmc/articles/PMC5804114/ /pubmed/29457028 http://dx.doi.org/10.1155/2017/4969076 Text en Copyright © 2017 Arianna Buccioni et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Buccioni, Arianna
Pallara, Grazia
Pastorelli, Roberta
Bellini, Letizia
Cappucci, Alice
Mannelli, Federica
Minieri, Sara
Roscini, Valentina
Rapaccini, Stefano
Mele, Marcello
Giovannetti, Luciana
Viti, Carlo
Pauselli, Mariano
Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes
title Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes
title_full Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes
title_fullStr Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes
title_full_unstemmed Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes
title_short Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes
title_sort effect of dietary chestnut or quebracho tannin supplementation on microbial community and fatty acid profile in the rumen of dairy ewes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5804114/
https://www.ncbi.nlm.nih.gov/pubmed/29457028
http://dx.doi.org/10.1155/2017/4969076
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