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Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1

BACKGROUND: Shikonin is a component of Chinese herbal medicine. The aim of this study was to investigate the effects of shikonin on cell migration of papillary thyroid cancer cells of the TPC-1 cell line in vitro and expression levels of the phosphate and tensin homolog deleted on chromosome 10 (PTE...

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Autores principales: Zhang, Yue, Sun, Bin, Huang, Zhi, Zhao, Dai-Wei, Zeng, Qingfan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5804303/
https://www.ncbi.nlm.nih.gov/pubmed/29389913
http://dx.doi.org/10.12659/MSM.908381
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author Zhang, Yue
Sun, Bin
Huang, Zhi
Zhao, Dai-Wei
Zeng, Qingfan
author_facet Zhang, Yue
Sun, Bin
Huang, Zhi
Zhao, Dai-Wei
Zeng, Qingfan
author_sort Zhang, Yue
collection PubMed
description BACKGROUND: Shikonin is a component of Chinese herbal medicine. The aim of this study was to investigate the effects of shikonin on cell migration of papillary thyroid cancer cells of the TPC-1 cell line in vitro and expression levels of the phosphate and tensin homolog deleted on chromosome 10 (PTEN) and DNA methyltransferase 1 (DNMT1) genes. MATERIAL/METHODS: The Cell Counting Kit-8 (CCK-8) assay was performed to evaluate the proliferation of TPC-1 papillary thyroid cancer cells, and the normal thyroid cells, HTori-3, in vitro. A transwell motility assay was used to analyze the migration of TPC-1 cells. Western blot was performed to determine the expression levels of PTEN and DNMT1 genes. A methylation-specific polymerase chain reaction (PCR) (MSP) assay was used to evaluate the methylation of PTEN. RESULTS: Following treatment with shikonin, the cell survival rate of TPC-1 cells decreased in a dose-dependent manner; the inhibitory effects on HTori-3 cells were less marked. Shikonin inhibited TPC-1 cell migration and invasion in a dose-dependent manner. The methylation of PTEN was suppressed by shikonin, which also reduced the expression of DNMT1 in a dose-dependent manner, and increased the expression of PTEN. Overexpression of DNMT1 promoted the migration of TPC-1 cells and the methylation of PTEN. Levels of protein expression of PTEN in TPC-1 cells treated with shikonin decreased, and were increased by DNMT1 knockdown. CONCLUSIONS: Shikonin suppressed the expression of DNMT1, reduced PTEN gene methylation, and increased PTEN protein expression, leading to the inhibition of TPC-1 cell migration.
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spelling pubmed-58043032018-02-12 Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1 Zhang, Yue Sun, Bin Huang, Zhi Zhao, Dai-Wei Zeng, Qingfan Med Sci Monit Lab/In Vitro Research BACKGROUND: Shikonin is a component of Chinese herbal medicine. The aim of this study was to investigate the effects of shikonin on cell migration of papillary thyroid cancer cells of the TPC-1 cell line in vitro and expression levels of the phosphate and tensin homolog deleted on chromosome 10 (PTEN) and DNA methyltransferase 1 (DNMT1) genes. MATERIAL/METHODS: The Cell Counting Kit-8 (CCK-8) assay was performed to evaluate the proliferation of TPC-1 papillary thyroid cancer cells, and the normal thyroid cells, HTori-3, in vitro. A transwell motility assay was used to analyze the migration of TPC-1 cells. Western blot was performed to determine the expression levels of PTEN and DNMT1 genes. A methylation-specific polymerase chain reaction (PCR) (MSP) assay was used to evaluate the methylation of PTEN. RESULTS: Following treatment with shikonin, the cell survival rate of TPC-1 cells decreased in a dose-dependent manner; the inhibitory effects on HTori-3 cells were less marked. Shikonin inhibited TPC-1 cell migration and invasion in a dose-dependent manner. The methylation of PTEN was suppressed by shikonin, which also reduced the expression of DNMT1 in a dose-dependent manner, and increased the expression of PTEN. Overexpression of DNMT1 promoted the migration of TPC-1 cells and the methylation of PTEN. Levels of protein expression of PTEN in TPC-1 cells treated with shikonin decreased, and were increased by DNMT1 knockdown. CONCLUSIONS: Shikonin suppressed the expression of DNMT1, reduced PTEN gene methylation, and increased PTEN protein expression, leading to the inhibition of TPC-1 cell migration. International Scientific Literature, Inc. 2018-02-01 /pmc/articles/PMC5804303/ /pubmed/29389913 http://dx.doi.org/10.12659/MSM.908381 Text en © Med Sci Monit, 2018 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Lab/In Vitro Research
Zhang, Yue
Sun, Bin
Huang, Zhi
Zhao, Dai-Wei
Zeng, Qingfan
Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1
title Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1
title_full Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1
title_fullStr Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1
title_full_unstemmed Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1
title_short Shikonin Inhibites Migration and Invasion of Thyroid Cancer Cells by Downregulating DNMT1
title_sort shikonin inhibites migration and invasion of thyroid cancer cells by downregulating dnmt1
topic Lab/In Vitro Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5804303/
https://www.ncbi.nlm.nih.gov/pubmed/29389913
http://dx.doi.org/10.12659/MSM.908381
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