Potential application of rLc36 protein for diagnosis of canine visceral leishmaniasis

Visceral leishmaniasis (VL) is fatal if left untreated. Infected dogs are important reservoirs of the disease, and thus specific identification of infected animals is very important. Several diagnostic tests have been developed for canine VL (CVL); however, these tests show varied specificity and se...

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Detalles Bibliográficos
Autores principales: Nogueira, Camila Tita, Cistia, Mayara Lúcia Del, Urbaczek, Ana Carolina, Jusi, Márcia MG, Velásquez, Angela Maria Arenas, Machado, Rosângela Zacarias, Ferreira, Henrique, Henrique-Silva, Flávio, Langoni, Hélio, da Costa, Paulo Inácio, Graminha, Márcia AS
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2018
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5804312/
https://www.ncbi.nlm.nih.gov/pubmed/29412359
http://dx.doi.org/10.1590/0074-02760170171
Descripción
Sumario:Visceral leishmaniasis (VL) is fatal if left untreated. Infected dogs are important reservoirs of the disease, and thus specific identification of infected animals is very important. Several diagnostic tests have been developed for canine VL (CVL); however, these tests show varied specificity and sensitivity. The present study describes the recombinant protein rLc36, expressed by Leishmania infantum, as potential antigen for more sensitive and specific diagnosis of CVL based on an immunoenzymatic assay. The concentration of 1.0 μg/mL of rLc36 enabled differentiation of positive and negative sera and showed a sensitivity of 85% and specificity of 71% (with 95% confidence), with an accuracy of 76%.

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