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Radiosensitization by CpG ODN7909 in an epidermoid laryngeal carcinoma Hep-2 cell line
OBJECTIVE: To evaluate the radiosensitivity effect of CpG oligodeoxyribonucleotide (ODN) 7909 on human epidermoid cancer strain-2 (Hep-2) cells in vitro and discuss the potential for improved radiotherapy treatment in patients with laryngeal squamous cell carcinoma. METHODS: Toll-like receptor (TLR)...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805225/ https://www.ncbi.nlm.nih.gov/pubmed/29239279 http://dx.doi.org/10.1177/0300060517728634 |
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author | Wang, Shu Liu, Xiaoqun Qiao, Tiankui Zhang, Qi |
author_facet | Wang, Shu Liu, Xiaoqun Qiao, Tiankui Zhang, Qi |
author_sort | Wang, Shu |
collection | PubMed |
description | OBJECTIVE: To evaluate the radiosensitivity effect of CpG oligodeoxyribonucleotide (ODN) 7909 on human epidermoid cancer strain-2 (Hep-2) cells in vitro and discuss the potential for improved radiotherapy treatment in patients with laryngeal squamous cell carcinoma. METHODS: Toll-like receptor (TLR) 9 expression was assessed in Hep-2 cells using Western blots and reverse transcription polymerase chain reaction. Cell Counting Kit-8 was used to detect Hep-2 cell viability at 24 and 48 h following treatment with different CpG ODN7909 concentrations. Cellular colonization was evaluated using microscopy. Cell cycle distribution and apoptosis rate was determined with flow cytometry. Interleukin (IL)-12 and tumour necrosis factor (TNF)-α concentrations were detected by enzyme-linked immunosorbent assay. RESULTS: Hep-2 cells were found to express TLR9, and CpG ODN7909 treatment suppressed Hep-2 cell viability in a dose- and time-dependent manner. Cell survival curve analyses revealed a sensitivity enhancement ratio of the mean death dose of 1.225 for CpG ODN7909 plus irradiation versus irradiation alone. Furthermore, the population of Gap 2/mitotic-phase cells, apoptosis rate and secreted IL-12 and TNF-α levels were significantly increased in Hep-2 cells treated with CpG ODN7909 plus irradiation versus IR alone. CONCLUSION: CpG ODN7909 enhanced the radiosensitivity of Hep-2 cells in vitro. |
format | Online Article Text |
id | pubmed-5805225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-58052252018-02-14 Radiosensitization by CpG ODN7909 in an epidermoid laryngeal carcinoma Hep-2 cell line Wang, Shu Liu, Xiaoqun Qiao, Tiankui Zhang, Qi J Int Med Res Research Report OBJECTIVE: To evaluate the radiosensitivity effect of CpG oligodeoxyribonucleotide (ODN) 7909 on human epidermoid cancer strain-2 (Hep-2) cells in vitro and discuss the potential for improved radiotherapy treatment in patients with laryngeal squamous cell carcinoma. METHODS: Toll-like receptor (TLR) 9 expression was assessed in Hep-2 cells using Western blots and reverse transcription polymerase chain reaction. Cell Counting Kit-8 was used to detect Hep-2 cell viability at 24 and 48 h following treatment with different CpG ODN7909 concentrations. Cellular colonization was evaluated using microscopy. Cell cycle distribution and apoptosis rate was determined with flow cytometry. Interleukin (IL)-12 and tumour necrosis factor (TNF)-α concentrations were detected by enzyme-linked immunosorbent assay. RESULTS: Hep-2 cells were found to express TLR9, and CpG ODN7909 treatment suppressed Hep-2 cell viability in a dose- and time-dependent manner. Cell survival curve analyses revealed a sensitivity enhancement ratio of the mean death dose of 1.225 for CpG ODN7909 plus irradiation versus irradiation alone. Furthermore, the population of Gap 2/mitotic-phase cells, apoptosis rate and secreted IL-12 and TNF-α levels were significantly increased in Hep-2 cells treated with CpG ODN7909 plus irradiation versus IR alone. CONCLUSION: CpG ODN7909 enhanced the radiosensitivity of Hep-2 cells in vitro. SAGE Publications 2017-09-18 2017-12 /pmc/articles/PMC5805225/ /pubmed/29239279 http://dx.doi.org/10.1177/0300060517728634 Text en © The Author(s) 2017 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Research Report Wang, Shu Liu, Xiaoqun Qiao, Tiankui Zhang, Qi Radiosensitization by CpG ODN7909 in an epidermoid laryngeal carcinoma Hep-2 cell line |
title | Radiosensitization by CpG ODN7909 in an epidermoid laryngeal
carcinoma Hep-2 cell line |
title_full | Radiosensitization by CpG ODN7909 in an epidermoid laryngeal
carcinoma Hep-2 cell line |
title_fullStr | Radiosensitization by CpG ODN7909 in an epidermoid laryngeal
carcinoma Hep-2 cell line |
title_full_unstemmed | Radiosensitization by CpG ODN7909 in an epidermoid laryngeal
carcinoma Hep-2 cell line |
title_short | Radiosensitization by CpG ODN7909 in an epidermoid laryngeal
carcinoma Hep-2 cell line |
title_sort | radiosensitization by cpg odn7909 in an epidermoid laryngeal
carcinoma hep-2 cell line |
topic | Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805225/ https://www.ncbi.nlm.nih.gov/pubmed/29239279 http://dx.doi.org/10.1177/0300060517728634 |
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