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Immunophenotypic characterization of human T cells after in vitro exposure to different silicone breast implant surfaces

The most common complication of silicone breast implants is capsular contracture (massive scar formation around the implant). We postulate that capsular contracture is always a sequel to inflammatory processes, with both innate and adaptive immune mechanisms participating. In general, fibroblasts an...

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Autores principales: Cappellano, Giuseppe, Ploner, Christian, Lobenwein, Susanne, Sopper, Sieghart, Hoertnagl, Paul, Mayerl, Christina, Wick, Nikolaus, Pierer, Gerhard, Wick, Georg, Wolfram, Dolores
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805229/
https://www.ncbi.nlm.nih.gov/pubmed/29420643
http://dx.doi.org/10.1371/journal.pone.0192108
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author Cappellano, Giuseppe
Ploner, Christian
Lobenwein, Susanne
Sopper, Sieghart
Hoertnagl, Paul
Mayerl, Christina
Wick, Nikolaus
Pierer, Gerhard
Wick, Georg
Wolfram, Dolores
author_facet Cappellano, Giuseppe
Ploner, Christian
Lobenwein, Susanne
Sopper, Sieghart
Hoertnagl, Paul
Mayerl, Christina
Wick, Nikolaus
Pierer, Gerhard
Wick, Georg
Wolfram, Dolores
author_sort Cappellano, Giuseppe
collection PubMed
description The most common complication of silicone breast implants is capsular contracture (massive scar formation around the implant). We postulate that capsular contracture is always a sequel to inflammatory processes, with both innate and adaptive immune mechanisms participating. In general, fibroblasts and macrophages have been used as cell types to evaluate in vitro the biocompatibility of breast implant surfaces. Moreover, also T cells have been found at the implant site at the initial stage of fibrous capsule formation. However, only few studies have addressed the influence of surfaces with different textures on T-cell responses. The aim of the present study was to investigate the immune response of human peripheral blood mononuclear cells (PBMC) to commercially available silicone breast implants in vitro. PBMC from healthy female blood donors were cultured on each silicone surface for 4 days. Proliferation and phenotype of cultured cells were assessed by flow cytometry. Cytokine levels were determined by multiplex and real-time assay. We found that silicone surfaces do not induce T-cell proliferation, nor do they extensively alter the proportion of T cell subsets (CD4, CD8, naïve, effector memory). Interestingly, cytokine profiling identified matrix specific differences, especially for IL-6 and TNF-α on certain surface topographies that could lead to increased fibrosis.
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spelling pubmed-58052292018-02-23 Immunophenotypic characterization of human T cells after in vitro exposure to different silicone breast implant surfaces Cappellano, Giuseppe Ploner, Christian Lobenwein, Susanne Sopper, Sieghart Hoertnagl, Paul Mayerl, Christina Wick, Nikolaus Pierer, Gerhard Wick, Georg Wolfram, Dolores PLoS One Research Article The most common complication of silicone breast implants is capsular contracture (massive scar formation around the implant). We postulate that capsular contracture is always a sequel to inflammatory processes, with both innate and adaptive immune mechanisms participating. In general, fibroblasts and macrophages have been used as cell types to evaluate in vitro the biocompatibility of breast implant surfaces. Moreover, also T cells have been found at the implant site at the initial stage of fibrous capsule formation. However, only few studies have addressed the influence of surfaces with different textures on T-cell responses. The aim of the present study was to investigate the immune response of human peripheral blood mononuclear cells (PBMC) to commercially available silicone breast implants in vitro. PBMC from healthy female blood donors were cultured on each silicone surface for 4 days. Proliferation and phenotype of cultured cells were assessed by flow cytometry. Cytokine levels were determined by multiplex and real-time assay. We found that silicone surfaces do not induce T-cell proliferation, nor do they extensively alter the proportion of T cell subsets (CD4, CD8, naïve, effector memory). Interestingly, cytokine profiling identified matrix specific differences, especially for IL-6 and TNF-α on certain surface topographies that could lead to increased fibrosis. Public Library of Science 2018-02-08 /pmc/articles/PMC5805229/ /pubmed/29420643 http://dx.doi.org/10.1371/journal.pone.0192108 Text en © 2018 Cappellano et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cappellano, Giuseppe
Ploner, Christian
Lobenwein, Susanne
Sopper, Sieghart
Hoertnagl, Paul
Mayerl, Christina
Wick, Nikolaus
Pierer, Gerhard
Wick, Georg
Wolfram, Dolores
Immunophenotypic characterization of human T cells after in vitro exposure to different silicone breast implant surfaces
title Immunophenotypic characterization of human T cells after in vitro exposure to different silicone breast implant surfaces
title_full Immunophenotypic characterization of human T cells after in vitro exposure to different silicone breast implant surfaces
title_fullStr Immunophenotypic characterization of human T cells after in vitro exposure to different silicone breast implant surfaces
title_full_unstemmed Immunophenotypic characterization of human T cells after in vitro exposure to different silicone breast implant surfaces
title_short Immunophenotypic characterization of human T cells after in vitro exposure to different silicone breast implant surfaces
title_sort immunophenotypic characterization of human t cells after in vitro exposure to different silicone breast implant surfaces
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805229/
https://www.ncbi.nlm.nih.gov/pubmed/29420643
http://dx.doi.org/10.1371/journal.pone.0192108
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