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The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus
Culture-independent methods were used to study the microbiota of adult worms, third-stage larvae and eggs, both in faeces and laid in vitro, of Haemonchus contortus, a nematode parasite of the abomasa of ruminants which is a major cause of production losses and ill-health. Bacteria were identified i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805237/ https://www.ncbi.nlm.nih.gov/pubmed/29420571 http://dx.doi.org/10.1371/journal.pone.0192164 |
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author | Sinnathamby, Gajenathirin Henderson, Gemma Umair, Saleh Janssen, Peter Bland, Ross Simpson, Heather |
author_facet | Sinnathamby, Gajenathirin Henderson, Gemma Umair, Saleh Janssen, Peter Bland, Ross Simpson, Heather |
author_sort | Sinnathamby, Gajenathirin |
collection | PubMed |
description | Culture-independent methods were used to study the microbiota of adult worms, third-stage larvae and eggs, both in faeces and laid in vitro, of Haemonchus contortus, a nematode parasite of the abomasa of ruminants which is a major cause of production losses and ill-health. Bacteria were identified in eggs, the female reproductive tract and the gut of adult and third-stage larvae (L3). PCR amplification of 16S rRNA sequences, denaturing gradient gel electrophoresis (DGGE) and clone libraries were used to compare the composition of the microbial communities of the different life-cycle stages of the parasites, as well as parasites and their natural environments. The microbiomes of adult worms and L3 were different from those in the abomasum or faeces respectively. The H. contortus microbiota was mainly comprised of members of the phyla Proteobacteria, Firmicutes and Bacteroidetes. Bacteria were localised in the gut, inside eggs and within the uterus of adult female worms using the universal FISH Eub338 probe, which targets most bacteria, and were also seen in these tissues by light and transmission electron microscopy. Streptococcus/Lactococcus sp. were identified within the distal uterus with the probe Strc493. Sequences from the genera Weissella and Leuconostoc were found in all life-cycle stages, except eggs collected from faeces, in which most sequences belonged to Clostridium sp. Bacteria affiliated with Weissella/Leuconostoc were identified in both PCR-DGGE short sequences and clone libraries of nearly full length 16S rRNA bacterial sequences in all life-cycle stages and subsequently visualised in eggs by fluorescent in situ hybridisation (FISH) with group-specific probes. This strongly suggests they are vertically transmitted endosymbionts. As this study was carried out on a parasite strain which has been maintained in the laboratory, other field isolates will need to be examined to establish whether these bacteria are more widely dispersed and have potential as targets to control H. contortus infections. |
format | Online Article Text |
id | pubmed-5805237 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58052372018-02-23 The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus Sinnathamby, Gajenathirin Henderson, Gemma Umair, Saleh Janssen, Peter Bland, Ross Simpson, Heather PLoS One Research Article Culture-independent methods were used to study the microbiota of adult worms, third-stage larvae and eggs, both in faeces and laid in vitro, of Haemonchus contortus, a nematode parasite of the abomasa of ruminants which is a major cause of production losses and ill-health. Bacteria were identified in eggs, the female reproductive tract and the gut of adult and third-stage larvae (L3). PCR amplification of 16S rRNA sequences, denaturing gradient gel electrophoresis (DGGE) and clone libraries were used to compare the composition of the microbial communities of the different life-cycle stages of the parasites, as well as parasites and their natural environments. The microbiomes of adult worms and L3 were different from those in the abomasum or faeces respectively. The H. contortus microbiota was mainly comprised of members of the phyla Proteobacteria, Firmicutes and Bacteroidetes. Bacteria were localised in the gut, inside eggs and within the uterus of adult female worms using the universal FISH Eub338 probe, which targets most bacteria, and were also seen in these tissues by light and transmission electron microscopy. Streptococcus/Lactococcus sp. were identified within the distal uterus with the probe Strc493. Sequences from the genera Weissella and Leuconostoc were found in all life-cycle stages, except eggs collected from faeces, in which most sequences belonged to Clostridium sp. Bacteria affiliated with Weissella/Leuconostoc were identified in both PCR-DGGE short sequences and clone libraries of nearly full length 16S rRNA bacterial sequences in all life-cycle stages and subsequently visualised in eggs by fluorescent in situ hybridisation (FISH) with group-specific probes. This strongly suggests they are vertically transmitted endosymbionts. As this study was carried out on a parasite strain which has been maintained in the laboratory, other field isolates will need to be examined to establish whether these bacteria are more widely dispersed and have potential as targets to control H. contortus infections. Public Library of Science 2018-02-08 /pmc/articles/PMC5805237/ /pubmed/29420571 http://dx.doi.org/10.1371/journal.pone.0192164 Text en © 2018 Sinnathamby et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Sinnathamby, Gajenathirin Henderson, Gemma Umair, Saleh Janssen, Peter Bland, Ross Simpson, Heather The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus |
title | The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus |
title_full | The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus |
title_fullStr | The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus |
title_full_unstemmed | The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus |
title_short | The bacterial community associated with the sheep gastrointestinal nematode parasite Haemonchus contortus |
title_sort | bacterial community associated with the sheep gastrointestinal nematode parasite haemonchus contortus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805237/ https://www.ncbi.nlm.nih.gov/pubmed/29420571 http://dx.doi.org/10.1371/journal.pone.0192164 |
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