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Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells
Tumor necrosis factor-alpha is a critical pro-inflammatory cytokine produced by macrophages and was once considered an anti-tumor agent. However, a low dose of tumor necrosis factor-alpha can cause epithelial mesenchymal transition, angiogenesis and metastasis. NF-κΒ contributes to epithelial mesenc...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805513/ https://www.ncbi.nlm.nih.gov/pubmed/29467927 http://dx.doi.org/10.18632/oncotarget.23645 |
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author | Shi, Guiling Zheng, Xiaoli Zhang, Shiwu Wu, Xiaojing Yu, Fei Wang, Yijia Xing, Fei |
author_facet | Shi, Guiling Zheng, Xiaoli Zhang, Shiwu Wu, Xiaojing Yu, Fei Wang, Yijia Xing, Fei |
author_sort | Shi, Guiling |
collection | PubMed |
description | Tumor necrosis factor-alpha is a critical pro-inflammatory cytokine produced by macrophages and was once considered an anti-tumor agent. However, a low dose of tumor necrosis factor-alpha can cause epithelial mesenchymal transition, angiogenesis and metastasis. NF-κΒ contributes to epithelial mesenchymal transition induced by tumor necrosis factor-alpha. Kanglaite, an extract from the Coix lacryma-jobi (adlay) seed, is an NF-κΒ inhibitor. The aim of this study was to investigate whether Kanglaite could inhibit epithelial mesenchymal transition caused by tumor necrosis factor-alpha using four colorectal cancer cell lines, HCT106, HCT116, LoVo and CT26. Our results showed that tumor necrosis factor-alpha -mediated activation of NF-κΒ, caused changes in epithelial mesenchymal transition -related protein expression, and increased migration and invasion in all four cell lines. However, these effects were inhibited by Kanglaite when used in combination with tumor necrosis factor-alpha. In a subcutaneous tumor model of CT26, tumor necrosis factor-alpha enhanced the tumorigenic ability of the cells, and again this was inhibited by Kanglaite. However, treatment with Kanglaite alone caused almost no inhibition of epithelial mesenchymal transition -mediated tumor growth, when cells were pretreated with tumor necrosis factor-alpha prior to injection. These results suggest that Kanglaite inhibits tumor necrosis factor-alpha -mediated epithelial mesenchymal transition in colorectal cancer cell lines via inhibition of NF-κΒ. |
format | Online Article Text |
id | pubmed-5805513 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-58055132018-02-21 Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells Shi, Guiling Zheng, Xiaoli Zhang, Shiwu Wu, Xiaojing Yu, Fei Wang, Yijia Xing, Fei Oncotarget Research Paper Tumor necrosis factor-alpha is a critical pro-inflammatory cytokine produced by macrophages and was once considered an anti-tumor agent. However, a low dose of tumor necrosis factor-alpha can cause epithelial mesenchymal transition, angiogenesis and metastasis. NF-κΒ contributes to epithelial mesenchymal transition induced by tumor necrosis factor-alpha. Kanglaite, an extract from the Coix lacryma-jobi (adlay) seed, is an NF-κΒ inhibitor. The aim of this study was to investigate whether Kanglaite could inhibit epithelial mesenchymal transition caused by tumor necrosis factor-alpha using four colorectal cancer cell lines, HCT106, HCT116, LoVo and CT26. Our results showed that tumor necrosis factor-alpha -mediated activation of NF-κΒ, caused changes in epithelial mesenchymal transition -related protein expression, and increased migration and invasion in all four cell lines. However, these effects were inhibited by Kanglaite when used in combination with tumor necrosis factor-alpha. In a subcutaneous tumor model of CT26, tumor necrosis factor-alpha enhanced the tumorigenic ability of the cells, and again this was inhibited by Kanglaite. However, treatment with Kanglaite alone caused almost no inhibition of epithelial mesenchymal transition -mediated tumor growth, when cells were pretreated with tumor necrosis factor-alpha prior to injection. These results suggest that Kanglaite inhibits tumor necrosis factor-alpha -mediated epithelial mesenchymal transition in colorectal cancer cell lines via inhibition of NF-κΒ. Impact Journals LLC 2017-12-22 /pmc/articles/PMC5805513/ /pubmed/29467927 http://dx.doi.org/10.18632/oncotarget.23645 Text en Copyright: © 2018 Shi et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Shi, Guiling Zheng, Xiaoli Zhang, Shiwu Wu, Xiaojing Yu, Fei Wang, Yijia Xing, Fei Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells |
title | Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells |
title_full | Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells |
title_fullStr | Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells |
title_full_unstemmed | Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells |
title_short | Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells |
title_sort | kanglaite inhibits emt caused by tnf-α via nf-κβ inhibition in colorectal cancer cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805513/ https://www.ncbi.nlm.nih.gov/pubmed/29467927 http://dx.doi.org/10.18632/oncotarget.23645 |
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