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Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells

Tumor necrosis factor-alpha is a critical pro-inflammatory cytokine produced by macrophages and was once considered an anti-tumor agent. However, a low dose of tumor necrosis factor-alpha can cause epithelial mesenchymal transition, angiogenesis and metastasis. NF-κΒ contributes to epithelial mesenc...

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Autores principales: Shi, Guiling, Zheng, Xiaoli, Zhang, Shiwu, Wu, Xiaojing, Yu, Fei, Wang, Yijia, Xing, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805513/
https://www.ncbi.nlm.nih.gov/pubmed/29467927
http://dx.doi.org/10.18632/oncotarget.23645
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author Shi, Guiling
Zheng, Xiaoli
Zhang, Shiwu
Wu, Xiaojing
Yu, Fei
Wang, Yijia
Xing, Fei
author_facet Shi, Guiling
Zheng, Xiaoli
Zhang, Shiwu
Wu, Xiaojing
Yu, Fei
Wang, Yijia
Xing, Fei
author_sort Shi, Guiling
collection PubMed
description Tumor necrosis factor-alpha is a critical pro-inflammatory cytokine produced by macrophages and was once considered an anti-tumor agent. However, a low dose of tumor necrosis factor-alpha can cause epithelial mesenchymal transition, angiogenesis and metastasis. NF-κΒ contributes to epithelial mesenchymal transition induced by tumor necrosis factor-alpha. Kanglaite, an extract from the Coix lacryma-jobi (adlay) seed, is an NF-κΒ inhibitor. The aim of this study was to investigate whether Kanglaite could inhibit epithelial mesenchymal transition caused by tumor necrosis factor-alpha using four colorectal cancer cell lines, HCT106, HCT116, LoVo and CT26. Our results showed that tumor necrosis factor-alpha -mediated activation of NF-κΒ, caused changes in epithelial mesenchymal transition -related protein expression, and increased migration and invasion in all four cell lines. However, these effects were inhibited by Kanglaite when used in combination with tumor necrosis factor-alpha. In a subcutaneous tumor model of CT26, tumor necrosis factor-alpha enhanced the tumorigenic ability of the cells, and again this was inhibited by Kanglaite. However, treatment with Kanglaite alone caused almost no inhibition of epithelial mesenchymal transition -mediated tumor growth, when cells were pretreated with tumor necrosis factor-alpha prior to injection. These results suggest that Kanglaite inhibits tumor necrosis factor-alpha -mediated epithelial mesenchymal transition in colorectal cancer cell lines via inhibition of NF-κΒ.
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spelling pubmed-58055132018-02-21 Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells Shi, Guiling Zheng, Xiaoli Zhang, Shiwu Wu, Xiaojing Yu, Fei Wang, Yijia Xing, Fei Oncotarget Research Paper Tumor necrosis factor-alpha is a critical pro-inflammatory cytokine produced by macrophages and was once considered an anti-tumor agent. However, a low dose of tumor necrosis factor-alpha can cause epithelial mesenchymal transition, angiogenesis and metastasis. NF-κΒ contributes to epithelial mesenchymal transition induced by tumor necrosis factor-alpha. Kanglaite, an extract from the Coix lacryma-jobi (adlay) seed, is an NF-κΒ inhibitor. The aim of this study was to investigate whether Kanglaite could inhibit epithelial mesenchymal transition caused by tumor necrosis factor-alpha using four colorectal cancer cell lines, HCT106, HCT116, LoVo and CT26. Our results showed that tumor necrosis factor-alpha -mediated activation of NF-κΒ, caused changes in epithelial mesenchymal transition -related protein expression, and increased migration and invasion in all four cell lines. However, these effects were inhibited by Kanglaite when used in combination with tumor necrosis factor-alpha. In a subcutaneous tumor model of CT26, tumor necrosis factor-alpha enhanced the tumorigenic ability of the cells, and again this was inhibited by Kanglaite. However, treatment with Kanglaite alone caused almost no inhibition of epithelial mesenchymal transition -mediated tumor growth, when cells were pretreated with tumor necrosis factor-alpha prior to injection. These results suggest that Kanglaite inhibits tumor necrosis factor-alpha -mediated epithelial mesenchymal transition in colorectal cancer cell lines via inhibition of NF-κΒ. Impact Journals LLC 2017-12-22 /pmc/articles/PMC5805513/ /pubmed/29467927 http://dx.doi.org/10.18632/oncotarget.23645 Text en Copyright: © 2018 Shi et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (http://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Shi, Guiling
Zheng, Xiaoli
Zhang, Shiwu
Wu, Xiaojing
Yu, Fei
Wang, Yijia
Xing, Fei
Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells
title Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells
title_full Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells
title_fullStr Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells
title_full_unstemmed Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells
title_short Kanglaite inhibits EMT caused by TNF-α via NF-κΒ inhibition in colorectal cancer cells
title_sort kanglaite inhibits emt caused by tnf-α via nf-κβ inhibition in colorectal cancer cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805513/
https://www.ncbi.nlm.nih.gov/pubmed/29467927
http://dx.doi.org/10.18632/oncotarget.23645
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