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Transplantation of schistosome sporocysts between host snails: A video guide
Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host an...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000 Research Limited
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5806052/ https://www.ncbi.nlm.nih.gov/pubmed/29487916 http://dx.doi.org/10.12688/wellcomeopenres.13488.1 |
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author | Mouahid, Gabriel Rognon, Anne de Carvalho Augusto, Ronaldo Driguez, Patrick Geyer, Kathy Karinshak, Shannon Luviano, Nelia Mann, Victoria Quack, Thomas Rawlinson, Kate Wendt, George Grunau, Christoph Moné, Hélène |
author_facet | Mouahid, Gabriel Rognon, Anne de Carvalho Augusto, Ronaldo Driguez, Patrick Geyer, Kathy Karinshak, Shannon Luviano, Nelia Mann, Victoria Quack, Thomas Rawlinson, Kate Wendt, George Grunau, Christoph Moné, Hélène |
author_sort | Mouahid, Gabriel |
collection | PubMed |
description | Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of ex vivo modifications, such as those used in functional genomics studies. |
format | Online Article Text |
id | pubmed-5806052 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | F1000 Research Limited |
record_format | MEDLINE/PubMed |
spelling | pubmed-58060522018-02-26 Transplantation of schistosome sporocysts between host snails: A video guide Mouahid, Gabriel Rognon, Anne de Carvalho Augusto, Ronaldo Driguez, Patrick Geyer, Kathy Karinshak, Shannon Luviano, Nelia Mann, Victoria Quack, Thomas Rawlinson, Kate Wendt, George Grunau, Christoph Moné, Hélène Wellcome Open Res Method Article Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of ex vivo modifications, such as those used in functional genomics studies. F1000 Research Limited 2018-01-09 /pmc/articles/PMC5806052/ /pubmed/29487916 http://dx.doi.org/10.12688/wellcomeopenres.13488.1 Text en Copyright: © 2018 Mouahid G et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Method Article Mouahid, Gabriel Rognon, Anne de Carvalho Augusto, Ronaldo Driguez, Patrick Geyer, Kathy Karinshak, Shannon Luviano, Nelia Mann, Victoria Quack, Thomas Rawlinson, Kate Wendt, George Grunau, Christoph Moné, Hélène Transplantation of schistosome sporocysts between host snails: A video guide |
title | Transplantation of
schistosome sporocysts between host snails: A video guide |
title_full | Transplantation of
schistosome sporocysts between host snails: A video guide |
title_fullStr | Transplantation of
schistosome sporocysts between host snails: A video guide |
title_full_unstemmed | Transplantation of
schistosome sporocysts between host snails: A video guide |
title_short | Transplantation of
schistosome sporocysts between host snails: A video guide |
title_sort | transplantation of
schistosome sporocysts between host snails: a video guide |
topic | Method Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5806052/ https://www.ncbi.nlm.nih.gov/pubmed/29487916 http://dx.doi.org/10.12688/wellcomeopenres.13488.1 |
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