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Transplantation of schistosome sporocysts between host snails: A video guide

Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host an...

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Autores principales: Mouahid, Gabriel, Rognon, Anne, de Carvalho Augusto, Ronaldo, Driguez, Patrick, Geyer, Kathy, Karinshak, Shannon, Luviano, Nelia, Mann, Victoria, Quack, Thomas, Rawlinson, Kate, Wendt, George, Grunau, Christoph, Moné, Hélène
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5806052/
https://www.ncbi.nlm.nih.gov/pubmed/29487916
http://dx.doi.org/10.12688/wellcomeopenres.13488.1
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author Mouahid, Gabriel
Rognon, Anne
de Carvalho Augusto, Ronaldo
Driguez, Patrick
Geyer, Kathy
Karinshak, Shannon
Luviano, Nelia
Mann, Victoria
Quack, Thomas
Rawlinson, Kate
Wendt, George
Grunau, Christoph
Moné, Hélène
author_facet Mouahid, Gabriel
Rognon, Anne
de Carvalho Augusto, Ronaldo
Driguez, Patrick
Geyer, Kathy
Karinshak, Shannon
Luviano, Nelia
Mann, Victoria
Quack, Thomas
Rawlinson, Kate
Wendt, George
Grunau, Christoph
Moné, Hélène
author_sort Mouahid, Gabriel
collection PubMed
description Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of ex vivo modifications, such as those used in functional genomics studies.
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spelling pubmed-58060522018-02-26 Transplantation of schistosome sporocysts between host snails: A video guide Mouahid, Gabriel Rognon, Anne de Carvalho Augusto, Ronaldo Driguez, Patrick Geyer, Kathy Karinshak, Shannon Luviano, Nelia Mann, Victoria Quack, Thomas Rawlinson, Kate Wendt, George Grunau, Christoph Moné, Hélène Wellcome Open Res Method Article Schistosomiasis is an important parasitic disease, touching roughly 200 million people worldwide. The causative agents are different Schistosoma species. Schistosomes have a complex life cycle, with a freshwater snail as intermediate host. After infection, sporocysts develop inside the snail host and give rise to human dwelling larvae. We present here a detailed step-by-step video instruction in English, French, Spanish and Portuguese that shows how these sporocysts can be manipulated and transferred from one snail to another. This procedure provides a technical basis for different types of ex vivo modifications, such as those used in functional genomics studies. F1000 Research Limited 2018-01-09 /pmc/articles/PMC5806052/ /pubmed/29487916 http://dx.doi.org/10.12688/wellcomeopenres.13488.1 Text en Copyright: © 2018 Mouahid G et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method Article
Mouahid, Gabriel
Rognon, Anne
de Carvalho Augusto, Ronaldo
Driguez, Patrick
Geyer, Kathy
Karinshak, Shannon
Luviano, Nelia
Mann, Victoria
Quack, Thomas
Rawlinson, Kate
Wendt, George
Grunau, Christoph
Moné, Hélène
Transplantation of schistosome sporocysts between host snails: A video guide
title Transplantation of schistosome sporocysts between host snails: A video guide
title_full Transplantation of schistosome sporocysts between host snails: A video guide
title_fullStr Transplantation of schistosome sporocysts between host snails: A video guide
title_full_unstemmed Transplantation of schistosome sporocysts between host snails: A video guide
title_short Transplantation of schistosome sporocysts between host snails: A video guide
title_sort transplantation of schistosome sporocysts between host snails: a video guide
topic Method Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5806052/
https://www.ncbi.nlm.nih.gov/pubmed/29487916
http://dx.doi.org/10.12688/wellcomeopenres.13488.1
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