Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay

The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F m...

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Autores principales: Senamela, Tshiphiri, Kock, Marleen, Becker, Piet, Potgieter, Joachim J.C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AOSIS 2018
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5806058/
https://www.ncbi.nlm.nih.gov/pubmed/29435426
http://dx.doi.org/10.4102/ajlm.v7i1.658
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author Senamela, Tshiphiri
Kock, Marleen
Becker, Piet
Potgieter, Joachim J.C.
author_facet Senamela, Tshiphiri
Kock, Marleen
Becker, Piet
Potgieter, Joachim J.C.
author_sort Senamela, Tshiphiri
collection PubMed
description The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation.
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spelling pubmed-58060582018-02-12 Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay Senamela, Tshiphiri Kock, Marleen Becker, Piet Potgieter, Joachim J.C. Afr J Lab Med Brief Report The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation. AOSIS 2018-01-31 /pmc/articles/PMC5806058/ /pubmed/29435426 http://dx.doi.org/10.4102/ajlm.v7i1.658 Text en © 2018. The Authors https://creativecommons.org/licenses/by/4.0/ Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.
spellingShingle Brief Report
Senamela, Tshiphiri
Kock, Marleen
Becker, Piet
Potgieter, Joachim J.C.
Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
title Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
title_full Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
title_fullStr Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
title_full_unstemmed Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
title_short Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
title_sort detection of the janus kinase 2 v617f mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5806058/
https://www.ncbi.nlm.nih.gov/pubmed/29435426
http://dx.doi.org/10.4102/ajlm.v7i1.658
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