Surfaces with Controllable Topography and Chemistry Used as a Template for Protein Crystallization

[Image: see text] Surfaces with controllable topography and chemistry were prepared to act as substrates for protein crystallization, in order to investigate the influence of these surface properties on the protein crystallization outcome. Three different methods were investigated to deposit 1,3,5-tris(10-carboxydecyloxy)benzene (TCDB) on a muscovite mica substrate to find the best route for controlled topography. Of these three, sublimation worked best. Contact angle measurements revealed that the surfaces with short exposure to the TCDB vapor (20 min or less) are hydrophilic, while surfaces exposed for 30 min or longer are hydrophobic. The hydrophilic surfaces are flat with low steps, while the hydrophobic surfaces contain macrosteps. Four model proteins were used for crystallization on the surfaces with controlled topography and chemistry. Hen egg white lysozyme crystals were less numerous on the surface with macrosteps than on smoother surfaces. On the other hand, insulin nucleated faster on the hydrophobic surfaces with macrosteps, and therefore, the crystals were more abundant and smaller. Bovine serum albumin and talin protein crystals were more numerous on all TCDB functionalized surfaces, compared to the reference clean muscovite mica surfaces. Overall, this shows that surface topography and chemistry is an important factor that partly determines the outcome in a protein crystallization experiment.